PETCM

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上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

PETCM  纯度: 99.36%

PETCM 是 caspase-3 的激活剂，以细胞色素 c (cyto c) 依赖的方式起作用。PETCM 可促进 Apaf-1 低聚化，可诱导 HeLa 细胞的凋亡 (apoptosis)。

PETCM

PETCM Chemical Structure

CAS No. : 10129-56-3

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5 mg ￥2100 In-stock
10 mg ￥3500 In-stock
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PETCM 相关产品

•相关化合物库:

  • Bioactive Compound Library Plus
  • Apoptosis Compound Library
  • Anti-Cancer Compound Library
  • Pyroptosis Compound Library

生物活性

PETCM is an activator of caspase-3 and acts as an cytochrome c (cyto c)-dependent manner. PETCM promotes Apaf-1 oligomerization and induces cell apoptosis in HeLa cells[1][2].

IC50 & Target[1]

Caspase 3

 

体外研究
(In Vitro)

PETCM (0.1-0.5 mM) stimulates caspase-3 activity (DEVD activity) of HeLa S-100 in a dose-dependent manner.And 0.2 mM PETCM is more efficient in activating caspase-3 than 1 mM dATP[1].
PETCM (0.2 mM; 1 hour) drives apoptosome formation. Apaf-1 in a normal HeLa cell S-100 fraction is in an inactive monomeric form. After 1 mM dATP, most of the Apaf-1 shifted to a size of 1 million daltons. After the S-100 fraction with 0.2 mM PETCM, Apaf-1 exhibits a similar shift. And the efficiency of apoptosome formation was better with 0.2 mM PETCM[1].
PETCM (0.2 mM; 1 hour) can antagonize the inhibitory activity of ProT reduced caspase-3 activation in in HeLa cells[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HeLa cells
Concentration: 0.2 mM
Incubation Time: 1 hour
Result: Increased apoptosome formation.

分子量

240.51

Formula

C8H8Cl3NO
CAS 号

10129-56-3
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
参考文献

  • [1]. Nguyen JT, et al. Direct activation of the apoptosis machinery as a mechanism to target cancer cells.Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7533-8.

    [2]. .Jiang X, Kim HE, Shu H, et al. Distinctive roles of PHAP proteins and prothymosin-alpha in a death regulatory pathway. Science, 2003, 299(5604): 223-226.

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