MIRA-1(Synonyms: NSC 19630)

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

MIRA-1 (Synonyms: NSC 19630)

MIRA-1 是一种马来酰亚胺类似物。MIRA-1 可通过恢复 p53 依赖的转录转激活诱导 p53 突变细胞凋亡 (apoptosis)。MIRA-1 具有抗肿瘤活性。

MIRA-1(Synonyms: NSC 19630)

MIRA-1 Chemical Structure

CAS No. : 72835-26-8

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

MIRA-1 is a maleimide analogue. MIRA-1 can induce apoptosis in mutant p53 cells via restoration of p53-dependent transcriptional transactivation. MIRA-1 has anticancer activity[1].

IC50 & Target

IC50: 10 μM (Saos-2 His273)[1]
体外研究
(In Vitro)

MIRA-1 suppresses growth of Saos-2-His273 cells expressing mutant p53[1].
MIRA-1 (25 μM; 48 hours) inhibits cell growth in a mutant p53-dependent manner[1].
MIRA-1 (5 μM; 14 days) dramatically reduces the number of colonies formed by His273-expressing Saos-2 cells, but is much less efficient in inhibiting p53-null Saos-2 cells[1].
MIRA-1 (10 μM; 48 hours) causes a substantial increase in the fraction of Saos-2 and Saos-2-His273 cells with a sub-G1 DNA content in the presence of mutant p53[1].
MIRA-1 (5 and 10 μM; 24 hours) induces EGFP expression, MDM2 and Bax in SKOV-His175 cells[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line: Saos-2-His273, H1299-His175, SKOV-His175 and SKOV-His273[1]
Concentration: 25 μM
Incubation Time: 48 hours
Result: Inhibited cell growth in a mutant p53-dependent manner, with survival rate of 18.5~39% in no doxycycline and 71.5~87.5% in doxycycline.

Apoptosis Analysis

Cell Line: Saos-2 and Saos-2-His273 cells[1]
Concentration: 10 μM
Incubation Time: 48 hours
Result: Caused a substantial increase in the fraction of cells with a sub-G1 DNA content in the presence of mutant p53.

分子量

183.16

Formula

C8H9NO4
CAS 号

72835-26-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Bykov VJ, et al. Reactivation of mutant p53 and induction of apoptosis in human tumor cells by maleimide analogs [published correction appears in J Biol Chem. 2017 Dec 1;292(48):19607]. J Biol Chem. 2005;280(34):30384-30391.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务