Sal003

发表于由

Sal003  纯度: 99.75%

Sal003 是高效、特异性的细胞渗透性 eIF2α 磷酸酶抑制剂。Sal003 是 salubrinal 的衍生物。

Sal003

Sal003 Chemical Structure

CAS No. : 1164470-53-4

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg)   Apply now  
10 mM * 1 mL in DMSO ￥770 In-stock
5 mg ￥700 In-stock
10 mg ￥1100 In-stock
50 mg ￥4500 In-stock
100 mg   询价  
200 mg   询价  

* Please select Quantity before adding items.

Sal003 相关产品

•相关化合物库:

  • Covalent Screening Library Plus
  • Bioactive Compound Library Plus
  • Apoptosis Compound Library
  • Metabolism/Protease Compound Library
  • Anti-Cancer Compound Library
  • Covalent Screening Library
  • Endoplasmic Reticulum Stress Compound Library
  • Phosphatase Inhibitor Library
  • Targeted Diversity Library

生物活性

Sal003 is a potent, specific and cell-permeable inhibitor of the eukaryotic translation initiation factor 2α (eIF2α) phosphatase. Sal003 is a derivative of salubrinal[1].

IC50 & Target

eIF2α phosphatase[1]
体外研究
(In Vitro)

Sal003 (20 μM; 1-12 hours) sharply increases eIF2α phosphorylation in mouse embryonic fibroblasts (MEFs)[2].
Eukaryotic translation initiation factor 2α (eIF2α) phosphorylation by Sal003 (10 μM; 1 hour) enhances subtilase cytotoxin (SubAB)-induced apoptotic signaling[1].
Sal003 promotes eIF2α phosphorylation leads to impairment of synaptic plasticity and memory[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis[2]

Cell Line: HeLa cells
Concentration: 10 μM
Incubation Time: 1 hour
Result: Phosphorylated eIF2α and thus enhanced SubAB-induced apoptotic signaling.

Western Blot Analysis[1]

Cell Line: Mouse embryonic fibroblasts (MEFs)
Concentration: 20 μM
Incubation Time: 1 hour, 3 hours, 6 hours, 12 hours
Result: Sharply increased eIF2α phosphorylation in mouse MEFs.

体内研究
(In Vivo)

Sal003 (20Μm; intrahippocampal injection; 8 minutes) impairs contextual memory in vivo[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Rats (300-325g)[1]
Dosage: 20 μM
Administration: Intrahippocampal injection; 8 minutes
Result: Impaired contextual memory.

分子量

463.21

Formula

C18H15Cl4N3OS
CAS 号

1164470-53-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : ≥ 100 mg/mL (215.88 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1588 mL 10.7942 mL 21.5885 mL
5 mM 0.4318 mL 2.1588 mL 4.3177 mL
10 mM 0.2159 mL 1.0794 mL 2.1588 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (5.40 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (5.40 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (5.40 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.40 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Costa-Mattioli M, et al. eIF2alpha phosphorylation bidirectionally regulates the switch from short- to long-term synaptic plasticity and memory. Cell. 2007 Apr 6;129(1):195-206.

    [2]. Yahiro K, et al. Regulation of subtilase cytotoxin-induced cell death by an RNA-dependent protein kinase-like endoplasmic reticulum kinase-dependent proteasome pathway in HeLa cells. Infect Immun. 2012 May;80(5):1803-14.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务