PAIR2 是一种有效和选择性的 IRE1α RNase 的部分拮抗剂。PAIR2 可以完全占据细胞中 IRE1α 的 ATP 结合位点,并阻断强效 KIRA 抑制 XBP1 剪接的能力。
PAIR2 Chemical Structure
规格
是否有货
100 mg
询价
250 mg
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500 mg
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生物活性
PAIR2 is a potent and selective partial antagonist of IRE1α RNase. PAIR2 can completely occupy IRE1α’s ATP-binding site in cells and block the ability of a potent KIRA to inhibit XBP1 splicing[1].
分子量
590.59
Formula
C27H26F4N6O3S
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Feldman HC, et, al. ATP-competitive partial antagonists of the IRE1α RNase segregate outputs of the UPR. Nat Chem Biol. 2021 Nov;17(11):1148-1156.
APY29 是 ATP 竞争性抑制剂,一种特异性的 IRE1α 别构调节剂,通过结合到 ATP 结合口袋来抑制 IRE1α 的自主磷酸化,IC50 值为 280 nM。APY29 也可以变构激活 IRE1α 相邻 RNase 域。
APY29 Chemical Structure
CAS No. : 1216665-49-4
规格
价格
是否有货
数量
10 mM * 1 mL in DMSO
¥1535
In-stock
10 mg
¥1395
In-stock
50 mg
¥6045
In-stock
100 mg
询价
200 mg
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* Please select Quantity before adding items.
APY29 相关产品
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生物活性
APY29, an ATP-competitive inhibitor, is an allosteric modulator of IRE1α which inhibits IRE1α autophosphorylation by binding to the ATP-binding pocket with IC50 of 280 nM. APY29 acts as a ligand that allosterically activates IRE1α adjacent RNase domain[1].
IC50 & Target
IC50: 280 nM (IRE1α)
体外研究 (In Vitro)
APY29 divergently modulates the RNase activity and oligomerization state of IRE1α. APY29 is exerting their opposing effects on RNase activity through the same binding site. APY29 divergently affects IRE1α oligomerization. APY29 demonstrates opposing dose-dependent effects on ER stress-induced activation of the RNase of endogenous IRE1α[1].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
332.36
Formula
C17H16N8
CAS 号
1216665-49-4
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-20°C
3 years
4°C
2 years
In solvent
-80°C
6 months
-20°C
1 month
溶解性数据
In Vitro:
DMSO : 53.33 mg/mL (160.46 mM; Need ultrasonic)
H2O : 5 mg/mL (15.04 mM; ultrasonic and adjust pH to 3 with HCl)
GSK2850163 is a novel inhibitor of inositol-requiring enzyme-1 alpha (IRE1α) which can inhibit IRE1α kinase activity and RNase activity with IC50s of 20 and 200 nM, respectively.
GSK2850163 is a novel inhibitor of inositol-requiring enzyme-1 alpha (IRE1α) which can inhibit IRE1α kinase activity and RNase activity with IC50s of 20 and 200 nM, respectively. The increased autophosphorylation of IRE1α could be reduced in a dose-dependent manner by GSK2850163. Increasing concentrations of GSK2850163 are capable of reducing the increased XBP 1 transcriptional activity. Two additional kinases are weakly inhibited by GSK2850163: Ron (IC50=4.4 μM) and FGFR1 V561M (IC50=17 μM)[1].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
446.41
Formula
C24H29Cl2N3O
CAS 号
2121989-91-9
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Nestor O. Concha, et al. Long-Range Inhibitor-Induced Conformational Regulation of Human IRE1α Endoribonuclease Activity. Molecular Pharmacology December 2015, 88 (6) 1011-1023.
Cell Assay [1]
PANC-1 cells are seeded into six-well plates at a density of 5.0×103 cells/well in RPMI 1640 media containing 10% FBS. Cells are cotransfected with a pGL3-5x unfolded protein response element (UPRE)-luciferase reporter containing five repetitions of the XBP-1 DNA binding site and pRL-SV40 using the FuGENE6 transfection reagent. Forty-eight hours later, cells are treated with 2.5 mg/mL tunicamycin for 1 hour, followed by GSK2850163 treatment for 16 hours. Luciferase expression is measured using Dual-Glo Luciferase Assay kit and normalized to Renilla expression levels[1].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献
[1]. Nestor O. Concha, et al. Long-Range Inhibitor-Induced Conformational Regulation of Human IRE1α Endoribonuclease Activity. Molecular Pharmacology December 2015, 88 (6) 1011-1023.