DSPE-PEG-NHS Cat. No. PG2-DSNS-3k 3400 Da 100 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DSPE-PEG-NHS

Cat. No. PG2-DSNS-3k DSPE-PEG-NHS           Cat. No. PG2-DSNS-3k     3400 Da    100 mg
Specification 3400 Da
Unit Size 100 mg
Price $385.00

Qty Add to Cart

DSPE-PEG-NHS, MW 3400

Synonym: 1,2-Distearoyl-sn-Glycero-3-Phosphoethanolamine PEG NHS, NHS PEG DSPE, DSPE PEG Succinimidyl ester

 

DSPE PEG NHS is one of Nanocs reactive phospholipid PEG conjugates that can react with primary amine groups. DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) is an 18 carbon phospholipid that is highly hydrophobic. PEG backbone, on the other hand, offers good hydrophilicity and water solubility. Succinimidyl ester (NHS) on the other PEG terminal reacts with primary amine groups efficiently at pH 8~10. NHS PEG DSPE thus can be easily incorporated into liposome and other nanoparticles via the reaction of NHS and amine. DSPE PEG NHS is one of most commonly used reactive phospholipids to conjugate antibodies, proteins, peptides or other substrates to the surface of liposome and other lipid PEG nanoparticles. Pegylated phospholipids have longer blood circulation time and higher stability for encapsulated molecules. Nanocs has developed a comprehensive collection of chemical reactive phospholipid PEG products that have high purity, various molecular weights and excellent chemical reactivity. These lipid PEG conjugates demonstrate excellent amphilphilic properties and offer superior advantages for small and large molecule modification, formulation and delivery. 

Product Information:

  • Form: Solid;
  • Color: White;
  • Solubility: Soluble in chloroform, warm water;
  • Reactive group: Succinimidyl NHS Ester;
  • Reactive to: Primary amine group (-NH2);
  • Lipid type: C18, saturated;
  • Purity: >90%;

Storage Conditions:

  • Store at -20 0C. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Related Products
PG2-DSNS-2k , DSPE-PEG-NHS
PG2-AMDS-3k , DSPE PEG Amine, DSPE-PEG-NH2
PG2-DBDS-3k , DBCO PEG DSPE, DSPE PEG DBCO

DBCO PEG NHS Cat. No. PG2-DBNS-20k 20000 Da 20 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DBCO PEG NHS

Cat. No. PG2-DBNS-20k DBCO PEG NHS           Cat. No. PG2-DBNS-20k     20000 Da    20 mg
Specification 20000 Da
Unit Size 20 mg
Price $485.00

Qty Add to Cart

Description:

DBCO PEG NHS is one of Nanocs’ amine reactive PEG derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO (Dibenzylcyclooctyne) PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.

Physical Properties:

  • Off-white or light yellow solid;
  • Soluble in regular aqeous solution;
  • Functional group: DBCO;
  • Reactive toward: Azide (-N3):
  • PEG MW: 20000 Da;

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

  • Store at -20 0C.
Documents
  • SDS
  • DataSheet

DSPE-PEG-NHS Cat. No. PG2-DSNS-2k 2000 Da 100 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DSPE-PEG-NHS

Cat. No. PG2-DSNS-2k DSPE-PEG-NHS           Cat. No. PG2-DSNS-2k     2000 Da    100 mg
Specification 2000 Da
Unit Size 100 mg
Price $385.00

Qty Add to Cart

Synonym: 1,2-Distearoyl-sn-Glycero-3-Phosphoethanolamine PEG NHS, NHS PEG DSPE, DSPE PEG Succinimidyl ester, DSPE Polyethylene glycol NHS

DSPE PEG NHS is one of Nanocs reactive phospholipid PEG conjugates that can react with primary amine groups. DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) is an 18 carbon phospholipid that is highly hydrophobic. PEG backbone, on the other hand, offers good hydrophilicity and water solubility. Succinimidyl ester (NHS) on the other PEG terminal reacts with primary amine groups efficiently at pH 8~10. NHS PEG DSPE thus can be easily incorporated into liposome and other nanoparticles via the reaction of NHS and amine. DSPE PEG NHS is one of most commonly used reactive phospholipids to conjugate antibodies, proteins, peptides or other substrates to the surface of liposome and other lipid PEG nanoparticles. Pegylated phospholipids have longer blood circulation time and higher stability for encapsulated molecules. Nanocs has developed a comprehensive collection of chemical reactive phospholipid PEG products that have high purity, various molecular weights and excellent chemical reactivity. These lipid PEG conjugates demonstrate excellent amphilphilic properties and offer superior advantages for small and large molecule modification, formulation and delivery. 

Product Information:

  • Form: Solid;
  • Color: White;
  • Solubility: Soluble in chloroform, warm water;
  • Reactive group: Succinimidyl NHS Ester;
  • Reactive to: Primary amine group (-NH2);
  • Lipid type: C18, saturated;
  • Purity: >95%;

Storage Conditions:

  • Store at -20 0C. Avoid frequent thaw and freeze.

References:

Click here to check hundreds of publications using Nanocs PEGs.

Documents
  • SDS
  • DataSheet

Related Products
PG1-DS-5k , DSPE PEG, mPEG-DSPE
PG2-DSNS-5k , DSPE-PEG-NHS
PG2-DSNS-3k , DSPE-PEG-NHS
PG2-DSNS-2k , DSPE-PEG-NHS
PG2-DSNS-10k , DSPE-PEG-NHS
PG2-DSNS-1k , DSPE-PEG-NHS
PG2-DSNS-20k , DSPE-PEG-NHS
PG2-DSML-2k , DSPE PEG Maleimide, DSPE-PEG-MAL
PG2-AMDS-2k , DSPE PEG Amine, DSPE-PEG-NH2
PG2-AMDS-20k , DSPE PEG Amine, DSPE-PEG-NH2

DBCO PEG NHS Cat. No. PG2-DBNS-30k 30000 Da 20 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DBCO PEG NHS

Cat. No. PG2-DBNS-30k DBCO PEG NHS           Cat. No. PG2-DBNS-30k     30000 Da    20 mg
Specification 30000 Da
Unit Size 20 mg
Price $485.00

Qty Add to Cart

Description:

DBCO PEG NHS is one of Nanocs’ amine reactive PEG derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO (Dibenzylcyclooctyne) PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.

Physical Properties:

  • Off-white or light yellow solid;
  • Soluble in regular aqeous solution;
  • Functional group: DBCO;
  • Reactive toward: Azide (-N3):
  • PEG MW: 30000 Da;

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

  • Store at -20 0C.
Documents
  • SDS
  • DataSheet

DBCO-NHS ester 2

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

DBCO-NHS ester 2 

DBCO-NHS ester 2 是一种用于制备抗体偶联药物 (ADC) 的可降解 linker,是一种二苯基环辛酮 (DBCO) 衍生物,用于无铜 click 反应。

DBCO-NHS ester 2

DBCO-NHS ester 2 Chemical Structure

CAS No. : 1384870-47-6

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

DBCO-NHS ester 2 is a cleavable linker that is used for making antibody-drug conjugate (ADC). DBCO-NHS ester 2 is a derivative of Dibenzylcyclooctyne (DBCO) used in copper-free click chemistry[1].

分子量

430.45

Formula

C25H22N2O5

CAS 号

1384870-47-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

溶解性数据
In Vitro: 

DMSO : 14.29 mg/mL (33.20 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3232 mL 11.6158 mL 23.2315 mL
5 mM 0.4646 mL 2.3232 mL 4.6463 mL
10 mM 0.2323 mL 1.1616 mL 2.3232 mL

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.43 mg/mL (3.32 mM); Clear solution

    此方案可获得 ≥ 1.43 mg/mL (3.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 14.3 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献
  • [1]. Tang F, et al. Chemoenzymatic synthesis of glycoengineered IgG antibodies and glycosite-specific antibody-drug conjugates. Nat Protoc. 2017 Aug;12(8):1702-1721.

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

DSPE-PEG-NHS Cat. No. PG2-DSNS-10k 10000 Da 100 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DSPE-PEG-NHS

Cat. No. PG2-DSNS-10k DSPE-PEG-NHS           Cat. No. PG2-DSNS-10k     10000 Da    100 mg
Specification 10000 Da
Unit Size 100 mg
Price $385.00

Qty Add to Cart

DSPE-PEG-NHS, MW 10000

Synonym: 1,2-Distearoyl-sn-Glycero-3-Phosphoethanolamine PEG NHS, NHS PEG DSPE, DSPE PEG Succinimidyl ester

DSPE PEG NHS is one of Nanocs reactive phospholipid PEG conjugates that can react with primary amine groups. DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) is an 18 carbon phospholipid that is highly hydrophobic. PEG backbone, on the other hand, offers good hydrophilicity and water solubility. Succinimidyl ester (NHS) on the other PEG terminal reacts with primary amine groups efficiently at pH 8~10. NHS PEG DSPE thus can be easily incorporated into liposome and other nanoparticles via the reaction of NHS and amine. DSPE PEG NHS is one of most commonly used reactive phospholipids to conjugate antibodies, proteins, peptides or other substrates to the surface of liposome and other lipid PEG nanoparticles. Pegylated phospholipids have longer blood circulation time and higher stability for encapsulated molecules. Nanocs has developed a comprehensive collection of chemical reactive phospholipid PEG products that have high purity, various molecular weights and excellent chemical reactivity. These lipid PEG conjugates demonstrate excellent amphilphilic properties and offer superior advantages for small and large molecule modification, formulation and delivery. 

Product Information:

  • Form: Solid;
  • Color: White;
  • Solubility: Soluble in chloroform, warm water;
  • Reactive group: Succinimidyl NHS Ester;
  • Reactive to: Primary amine group (-NH2);
  • Lipid type: C18, saturated;
  • Purity: >90%;

Storage Conditions:

  • Store at -20 0C. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Related Products
PG2-DSNS-2k , DSPE-PEG-NHS
PG2-DSML-2k , DSPE PEG Maleimide, DSPE-PEG-MAL
PG2-BNDS-2k , DSPE PEG Biotin
PG2-AMDS-2k , DSPE PEG Amine, DSPE-PEG-NH2
PG2-CADS-2k , DSPE PEG Acid, DSPE-PEG-COOH
PG2-CADS-20k , DSPE PEG Acid, DSPE-PEG-COOH

DSPE-PEG-NHS Cat. No. PG2-DSNS-1k 1000 Da 100 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DSPE-PEG-NHS

Cat. No. PG2-DSNS-1k DSPE-PEG-NHS           Cat. No. PG2-DSNS-1k     1000 Da    100 mg
Specification 1000 Da
Unit Size 100 mg
Price $425.00

Qty Add to Cart

DSPE-PEG-NHS, MW 1000

Synonym:1,2-Distearoyl-sn-Glycero-3-Phosphoethanolamine PEG NHS, NHS PEG DSPE, DSPE PEG Succinimidyl ester

 

DSPE PEG NHS is one of Nanocs reactive phospholipid PEG conjugates that can react with primary amine groups. DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) is an 18 carbon phospholipid that is highly hydrophobic. PEG backbone, on the other hand, offers good hydrophilicity and water solubility. Succinimidyl ester (NHS) on the other PEG terminal reacts with primary amine groups efficiently at pH 8~10. NHS PEG DSPE thus can be easily incorporated into liposome and other nanoparticles via the reaction of NHS and amine. DSPE PEG NHS is one of most commonly used reactive phospholipids to conjugate antibodies, proteins, peptides or other substrates to the surface of liposome and other lipid PEG nanoparticles. Pegylated phospholipids have longer blood circulation time and higher stability for encapsulated molecules. Nanocs has developed a comprehensive collection of chemical reactive phospholipid PEG products that have high purity, various molecular weights and excellent chemical reactivity. These lipid PEG conjugates demonstrate excellent amphilphilic properties and offer superior advantages for small and large molecule modification, formulation and delivery. 

Product Information:

  • Form: Solid;
  • Color: White;
  • Solubility: Soluble in chloroform, warm water;
  • Reactive group: Succinimidyl NHS Ester;
  • Reactive to: Primary amine group (-NH2);
  • Lipid type: C18, saturated;
  • Purity: >90%;

Storage Conditions:

  • Store at -20 0C.  Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Related Products
PG2-DSNS-2k , DSPE-PEG-NHS
PG2-CADS-1k , DSPE PEG Acid, DSPE-PEG-COOH

DBCO-PEG4-NHS ester

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

DBCO-PEG4-NHS ester  纯度: 97.97%

DBCO-PEG4-NHS ester 是一种 PROTAC linker,属于 PEG 类。可用于合成 PROTAC 分子。

DBCO-PEG4-NHS ester

DBCO-PEG4-NHS ester Chemical Structure

CAS No. : 1427004-19-0

规格 价格 是否有货 数量
5 mg ¥1800 In-stock
10 mg ¥3000 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

生物活性

DBCO-PEG4-NHS ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1].

IC50 & Target

PEGs

 

体外研究
(In Vitro)

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

649.69

Formula

C34H39N3O10

CAS 号

1427004-19-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

溶解性数据
In Vitro: 

DMSO : 83.33 mg/mL (128.26 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.5392 mL 7.6960 mL 15.3920 mL
5 mM 0.3078 mL 1.5392 mL 3.0784 mL
10 mM 0.1539 mL 0.7696 mL 1.5392 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (3.20 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.20 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.08 mg/mL (3.20 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.08 mg/mL (3.20 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (3.20 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.20 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献
  • [1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

DSPE-PEG-NHS Cat. No. PG2-DSNS-20k 20000 Da 100 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DSPE-PEG-NHS

Cat. No. PG2-DSNS-20k DSPE-PEG-NHS           Cat. No. PG2-DSNS-20k     20000 Da    100 mg
Specification 20000 Da
Unit Size 100 mg
Price $425.00

Qty Add to Cart

DSPE-PEG-NHS, MW 20000

Synonym: 1,2-Distearoyl-sn-Glycero-3-Phosphoethanolamine PEG NHS, NHS PEG DSPE, DSPE PEG Succinimidyl ester

DSPE PEG NHS is one of Nanocs reactive phospholipid PEG conjugates that can react with primary amine groups. DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) is an 18 carbon phospholipid that is highly hydrophobic. PEG backbone, on the other hand, offers good hydrophilicity and water solubility. Succinimidyl ester (NHS) on the other PEG terminal reacts with primary amine groups efficiently at pH 8~10. NHS PEG DSPE thus can be easily incorporated into liposome and other nanoparticles via the reaction of NHS and amine. DSPE PEG NHS is one of most commonly used reactive phospholipids to conjugate antibodies, proteins, peptides or other substrates to the surface of liposome and other lipid PEG nanoparticles. Pegylated phospholipids have longer blood circulation time and higher stability for encapsulated molecules. Nanocs has developed a comprehensive collection of chemical reactive phospholipid PEG products that have high purity, various molecular weights and excellent chemical reactivity. These lipid PEG conjugates demonstrate excellent amphilphilic properties and offer superior advantages for small and large molecule modification, formulation and delivery. 

Product Information:

  • Form: Solid;
  • Color: White;
  • Solubility: Soluble in chloroform, warm water;
  • Reactive group: Succinimidyl NHS Ester;
  • Reactive to: Primary amine group (-NH2);
  • Lipid type: C18, saturated;
  • Purity: >90%;

Storage Conditions:

  • Store at -20 0C. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Related Products
PG2-DSNS-2k , DSPE-PEG-NHS
PG2-DSML-20k , DSPE PEG Maleimide, DSPE-PEG-MAL

DSPE PEG NHS, MW 5000 Cat. No. PG2-DSNS-5k-2 500 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DSPE PEG NHS, MW 5000

Cat. No. PG2-DSNS-5k-2 DSPE PEG NHS, MW 5000           Cat. No. PG2-DSNS-5k-2         500 mg
Specification
Unit Size 500 mg
Price $1,140.00

Qty Add to Cart

DSPE-PEG-NHS, MW 5000, 500 mg

Synonym: 1,2-Distearoyl-sn-Glycero-3-Phosphoethanolamine PEG NHS, NHS PEG DSPE, DSPE PEG Succinimidyl ester

DSPE PEG NHS is one of Nanocs reactive phospholipid PEG conjugates that can react with primary amine groups. DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) is an 18 carbon phospholipid that is highly hydrophobic. PEG backbone, on the other hand, offers good hydrophilicity and water solubility. Succinimidyl ester (NHS) on the other PEG terminal reacts with primary amine groups efficiently at pH 8~10. NHS PEG DSPE thus can be easily incorporated into liposome and other nanoparticles via the reaction of NHS and amine. DSPE PEG NHS is one of most commonly used reactive phospholipids to conjugate antibodies, proteins, peptides or other substrates to the surface of liposome and other lipid PEG nanoparticles. Pegylated phospholipids have longer blood circulation time and higher stability for encapsulated molecules. Nanocs has developed a comprehensive collection of chemical reactive phospholipid PEG products that have high purity, various molecular weights and excellent chemical reactivity. These lipid PEG conjugates demonstrate excellent amphilphilic properties and offer superior advantages for small and large molecule modification, formulation and delivery. 

Product Information:

  • Form: Solid;
  • Color: White;
  • Solubility: Soluble in chloroform, warm water;
  • Reactive group: Succinimidyl NHS Ester;
  • Reactive to: Primary amine group (-NH2);
  • Lipid type: C18, saturated;
  • Purity: >90%;

Storage Conditions:

  • Store at -20 0C. Avoid frequent thaw and freeze.
Related Products
PG2-DSNS-2k , DSPE-PEG-NHS
PG2-DSML-20k , DSPE PEG Maleimide, DSPE-PEG-MAL

Mal-PEG3-NHS ester

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Mal-PEG3-NHS ester  纯度: 98.75%

Mal-PEG3-NHS ester 是一种不可降解 (non-cleavable) 的 ADC linker,含 Maleimide 基团。可用于制备抗体偶联药物。

Mal-PEG3-NHS ester

Mal-PEG3-NHS ester Chemical Structure

CAS No. : 1537892-36-6

规格 价格 是否有货 数量
25 mg ¥1400 In-stock
50 mg ¥2400 In-stock
100 mg ¥3800 In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

Mal-PEG3-NHS ester is a noncleavable ADC linker containing a Maleimide group. Mal-PEG3-NHS ester is used for making antibody-drug conjugate[1].

IC50 & Target

Non-cleavable

 

体外研究
(In Vitro)

Maleimide-PEG-NHS ester, the heterobifunctional crosslinker, can be used to couple thiol-bearing ligands to amine-functionalized matrices[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

398.36

Formula

C17H22N2O9

CAS 号

1537892-36-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

参考文献
  • [1]. Gautam S, et al. Immobilization of hydrophobic peptidic ligands to hydrophilic chromatographic matrix: a preconcentration approach. Anal Biochem. 2012 Apr 15;423(2):202-9.

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

Bis-​PEG4-​NHS ester

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Bis-​PEG4-​NHS ester 

Bis-​PEG4-​NHS ester 是一种不可降解的含 4 个单元 PEG 的 linker,可用于合成抗体偶联药物 (ADC) 。

Bis-​PEG4-​NHS ester

Bis-​PEG4-​NHS ester Chemical Structure

CAS No. : 1314378-11-4

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

Bis-​PEG4-​NHS ester is a nonclaevable 4-unit PEG linker for antibody-drug-conjugation (ADC).

IC50 & Target

Non-cleavable

 

分子量

488.44

Formula

C20H28N2O12

CAS 号

1314378-11-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

Bis-PEG3-NHS ester

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Bis-PEG3-NHS ester 

Bis-PEG3-NHS ester 是一种不可降解的含 3 个单元 PEG 的 linker,可用于合成抗体偶联药物 (ADC) 。

Bis-PEG3-NHS ester

Bis-PEG3-NHS ester Chemical Structure

CAS No. : 1314378-16-9

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

Bis-PEG3-NHS ester is a nonclaevable 3-unit PEG linker for antibody-drug-conjugation (ADC).

IC50 & Target

Non-cleavable

 

分子量

444.39

Formula

C18H24N2O11

CAS 号

1314378-16-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

4-N3Pfp-NHS ester

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

4-N3Pfp-NHS ester 

4-N3Pfp-NHS ester 是一种不可降解 (non-cleavable) 的 ADC linker,用于抗体偶联药物 (ADCs) 的合成。

4-N3Pfp-NHS ester

4-N3Pfp-NHS ester Chemical Structure

CAS No. : 126695-58-7

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

4-N3Pfp-NHS ester is a noncleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs)[1].

IC50 & Target

Non-cleavable

 

分子量

332.17

Formula

C11H4F4N4O4

CAS 号

126695-58-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

Mal-amido-PEG10-C2-​NHS ester

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Mal-amido-PEG10-C2-​NHS ester  纯度: 95.23%

Mal-amido-PEG10-C2-NHS ester 是包含马来酰亚胺基团和 NHS 酯的,不可降解 (non-cleavable) 的 ADC linker。NHS ester 可用于标记蛋白质,胺修饰的寡核苷酸和其他含胺分子的伯胺 (-NH2)。

Mal-amido-PEG10-C2-​NHS ester

Mal-amido-PEG10-C2-​NHS ester Chemical Structure

CAS No. : 1137109-22-8

规格 价格 是否有货 数量
100 mg ¥13790 In-stock
500 mg ¥41380 询价
1 g ¥57470 询价
5 g   询价  
10 g   询价  

* Please select Quantity before adding items.

生物活性

Mal-amido-PEG10-C2-NHS ester is a nonclaevable ADC linker containing a maleimide group and an NHS ester. The NHS ester can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules[1][2].

IC50 & Target

Non-cleavable

 

分子量

777.81

Formula

C34H55N3O17

CAS 号

1137109-22-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Pure form -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 100 mg/mL (128.57 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2857 mL 6.4283 mL 12.8566 mL
5 mM 0.2571 mL 1.2857 mL 2.5713 mL
10 mM 0.1286 mL 0.6428 mL 1.2857 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (3.21 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.21 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.21 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.21 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (3.21 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.21 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献
  • [1]. Hansen AM, et al. Microwave-assisted solid-phase synthesis of antisense acpP peptide nucleic acid-peptide conjugates active against colistin- and tigecycline-resistant E. coli and K. pneumoniae. Eur J Med Chem. 2019 Apr 15;168:134-145.

    [2]. Zhang ge, et al. Preparation and application of FAPalpha activated polypeptide magnetic nanosphere compound used for diagnosis of tumors. CN105524141A

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

Mal-amido-PEG5-C2-​NHS ester

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

Mal-amido-PEG5-C2-​NHS ester 

Mal-amido-PEG10-C2-NHS ester 是包含马来酰亚胺基团和 NHS 酯的,不可降解 (non-cleavable) 的 ADC linker。NHS ester 可用于标记蛋白质,胺修饰的寡核苷酸和其他含胺分子的伯胺 (-NH2)。

Mal-amido-PEG5-C2-​NHS ester

Mal-amido-PEG5-C2-​NHS ester Chemical Structure

CAS No. : 1315355-92-0

规格 价格 是否有货
100 mg ¥11490 询问价格 & 货期

* Please select Quantity before adding items.

生物活性

Mal-amido-PEG10-C2-NHS ester is a nonclaevable ADC linker containing a maleimide group and an NHS ester. The NHS ester can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules[1][2]

IC50 & Target

Non-cleavable

 

分子量

557.55

Formula

C24H35N3O12

CAS 号

1315355-92-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Hansen AM, et al. Microwave-assisted solid-phase synthesis of antisense acpP peptide nucleic acid-peptide conjugates active against colistin- and tigecycline-resistant E. coli and K. pneumoniae. Eur J Med Chem. 2019 Apr 15;168:134-145.

    [2]. Zhang ge, et al. Preparation and application of FAPalpha activated polypeptide magnetic nanosphere compound used for diagnosis of tumors. CN105524141A

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

N3-C2-NHS ester

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

N3-C2-NHS ester 

N3-C2-NHS ester 是一种不可降解 (non-cleavable) 的 ADC linker,可用于合成抗体偶联药物 (ADCs)。

N3-C2-NHS ester

N3-C2-NHS ester Chemical Structure

CAS No. : 850180-76-6

规格 价格 是否有货
100 mg ¥4600 询问价格 & 货期
500 mg ¥13790 询问价格 & 货期
1 g ¥22990 询问价格 & 货期

* Please select Quantity before adding items.

生物活性

N3-C2-NHS ester is a noncleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs).

IC50 & Target

Non-cleavable

 

分子量

212.16

Formula

C7H8N4O4

CAS 号

850180-76-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Gregson SJ, et al. Pyrrolobenzodiazepine Dimer Antibody-Drug Conjugates: Synthesis and Evaluation of Noncleavable Drug-Linkers. J Med Chem. 2017 Dec 14;60(23):9490-9507.

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

N3-C3-NHS ester

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

N3-C3-NHS ester  纯度: 99.46%

N3-C3-NHS ester 是一种不可降解 (non-cleavable) 的 ADC linker,可用于合成抗体偶联药物 (ADCs)。

N3-C3-NHS ester

N3-C3-NHS ester Chemical Structure

CAS No. : 943858-70-6

规格 价格 是否有货 数量
100 mg ¥4600 In-stock
500 mg ¥13790 In-stock
1 g   询价  
5 g   询价  

* Please select Quantity before adding items.

生物活性

N3-C3-NHS ester is a noncleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs).

IC50 & Target

Non-cleavable

 

分子量

226.19

Formula

C8H10N4O4

CAS 号

943858-70-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 100 mg/mL (442.11 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.4211 mL 22.1053 mL 44.2106 mL
5 mM 0.8842 mL 4.4211 mL 8.8421 mL
10 mM 0.4421 mL 2.2105 mL 4.4211 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (11.05 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (11.05 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (11.05 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (11.05 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (11.05 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (11.05 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献
  • [1]. Pyrrolobenzodiazepine Dimer Antibody-Drug Conjugates: Synthesis and Evaluation of Noncleavable Drug-Linkers. J Med Chem. 2017 Dec 14;60(23):9490-9507.

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

N3-C4-NHS ester

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

N3-C4-NHS ester 

N3-C4-NHS ester 是一种不可降解 (non-cleavable) 的 ADC linker,可用于合成抗体偶联药物 (ADCs)。

N3-C4-NHS ester

N3-C4-NHS ester Chemical Structure

CAS No. : 478801-48-8

规格 价格 是否有货
100 mg ¥4600 询问价格 & 货期
500 mg ¥13790 询问价格 & 货期

* Please select Quantity before adding items.

生物活性

N3-C4-NHS ester is a noncleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs).

IC50 & Target

Non-cleavable

 

分子量

240.22

Formula

C9H12N4O4

CAS 号

478801-48-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Pyrrolobenzodiazepine Dimer Antibody-Drug Conjugates: Synthesis and Evaluation of Noncleavable Drug-Linkers. J Med Chem. 2017 Dec 14;60(23):9490-9507.

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

N3-C5-NHS ester

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

N3-C5-NHS ester  纯度: 98.04%

N3-C5-NHS ester 是一种不可降解 (non-cleavable) 的 ADC linker,可用于合成抗体偶联药物 (ADCs)。

N3-C5-NHS ester

N3-C5-NHS ester Chemical Structure

CAS No. : 866363-70-4

规格 价格 是否有货 数量
100 mg ¥4600 In-stock
500 mg ¥13790 In-stock
1 g   询价  
5 g   询价  

* Please select Quantity before adding items.

生物活性

N3-C5-NHS ester is a noncleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs).

IC50 & Target

Non-cleavable

 

分子量

254.24

Formula

C10H14N4O4

CAS 号

866363-70-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Pure form -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 100 mg/mL (393.33 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.9333 mL 19.6665 mL 39.3329 mL
5 mM 0.7867 mL 3.9333 mL 7.8666 mL
10 mM 0.3933 mL 1.9666 mL 3.9333 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (9.83 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (9.83 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (9.83 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (9.83 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (9.83 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (9.83 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献
  • [1]. Pyrrolobenzodiazepine Dimer Antibody-Drug Conjugates: Synthesis and Evaluation of Noncleavable Drug-Linkers. J Med Chem. 2017 Dec 14;60(23):9490-9507.

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