来自化脓性链球菌的链球菌溶血素O

Description

Used to create holes (reversible pores) in cholesterol-enriched plasma membranes of eukaryotic cells allowing selective permeabilization with molecules such as proteins (up to 100 kDa) and siRNAs.Streptolysin O is a toxin secreted byStreptococcus pyogenesand is a prototype member of poreforming bacterial cytolysins. It is used permeabilize cell membranes to permit cellular uptake of large or charged molecules. It is used to study macromolecule delivery. It is a potential anticancer agent and is used to study suicide cancer gene therapy.Permeabilizes membranes to permit cellular uptake of large or charged molecules.

Preparation instructions

Form

Lyophilized powder containing Tris buffer salts, sodium azide, PMSF, and EDTA.

Principle

嘌呤霉素 二盐酸盐 来源于白色链球菌

制备说明

This product is soluble in water at 50 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C. This product is also soluble in methanol at 10 mg/mL.

General Description

Puromycin is a broad spectrum antibiotic produced by Streptomyces alboniger . It is an analog of the 3′ end of aminoacyl-tRNA. It is an inhibitor of peptidyl transferase and is active in both prokaryotes and eukaryotes. Chemical structure: peptidyl nucleoside

Application

Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger . It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL. Puromycin dihydrochloride from Streptomyces alboniger has been used: in the preparation of puromycin stock solution for puromycin sensitivity assay. to select lentivirus-infected cells containing puromycin resistant selection marker. Used as a selective agent for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). Recommended for use at a range of 1-10 μg/ml. Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C.

Other Notes

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place

乳酸链球菌肽 来源于乳酸乳球菌

一般描述

化学结构：肽

应用

可用于在脂质II介导的细胞膜上形成目标孔。 可用于研究乳链菌肽-脂质II孔复合物的组装和稳定性。 乳链菌肽是由 乳酸乳球菌 产生。它通常用作食品防腐剂。它也被用作细胞培养物中的选择性试剂，用于分离革兰氏阴性细菌、酵母和霉菌。乳链菌肽已在生物测定中用于研究翻译后修饰。

其他说明

保存于密闭容器内，置于干燥通风处。置于干燥处保存。

General Description

Chemical structure: peptide

Application

Nisin is a polycyclic lantibiotic produced by Lactococcus lactis . It is commonly used as a food preservative. It is also used as a selective agent in cell culture for the isolation of gram-negative bacteria, yeast, and molds. Nisin has been used in bioassays to study posttranslational modification. Used to form targeted pores in cell membranes that are mediated by lipid II. Used to study the assembly and stability of nisin-lipid II pore complexes.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

乳酸链球菌肽 来源于乳酸乳球菌

Antibiotic with bactericidal action. Binds to the lipid A portion of bacterial lipopolysaccharides. Induces pore formation in the membranes of cortex cells from excised sorghum roots.Mode of Action: Binds to and interferes with the permeability of the cytoplasmic membrane.Antimicrobial spectrum: Gram-negative and Gram-positive bacteria (requires chelator such as EDTA).

Antibiotic with bactericidal action. Binds to the lipid A portion of bacterial lipopolysaccharides. Induces pore formation in the membranes of cortex cells from excised sorghum roots.Mode of Action: Binds to and interferes with the permeability of the cytoplasmic membrane.Antimicrobial spectrum: Gram-negative and Gram-positive bacteria (requires chelator such as EDTA).
An antibiotic with bactericidal action