Surface functional agarose beads from Nanocs have uniform size in the range of 50~150 microns. These activated beads have multiple functional groups that can be used to attached various bio-ligands covalently to the bead surface. Covalently linked ligands offer high stability and are suitable for various biomolecule purification, capturing and assay. Nanocs surface modified agarose beads provide more choices than any other companies in this field. Customer conjugation and modification is available upon request.
Specifications:
Functional group density: 25~40 umol/mL gel;
Loading density: 5~25 mg BSA/mL gel;
Composition: Suspension in aqueous solution, 20% ethanol;
N3PT(N3-pyridyl thiamine) is a potent and selective transketolase(TKT) inhibitor (IC50= 22 nM for Apo-TK) both in vitro and in vivo. IC50 Value: 22 nM( Apo-TK) ; 26 nM (EC50, Cellular TK) [1] Target: transketolase in vitro: N3PT inhibits transketolase activity in a cell based assay. Competitive inhibition of TK by N3PT in cells treated with increasing doses of thiamine, expressed as percentage enzymatic activity (the slope of initial linear range) of controls not treated with compounds [1]. in vivo: Tumors were induced in mice at day 0 and mice were then treated at day 7 with vehicle alone or with N3PT [2]. Low-thiamine diet enhances the sensitivity to N3PT inhibition of TK in spleen. Animals were switched to diets containing 16.5 mg/kg (unchanged), 5 mg/kg, 1 mg/kg, or 0 mg/kg thiamine, from a normal chow containing 16.5mg/kg thiamine [1].
分子量
336.28
Formula
C13H19Cl2N3OS
CAS 号
13860-66-7
运输条件
Room temperature in continental US; may vary elsewhere.
Solubility: 8 mg/mL (23.79 mM); Clear solution; Need ultrasonic
*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献
[1]. Allen A. Thomas, Josh Ballard, Bryan Bernat. Potent and Selective Thiamine Antagonists That Inhibit Transketolase.
[2]. Jeno Gyuris, May Han, Ronan C, N3-pyridyl-thiamine and its use in cancer treatments. Patent Numeber: WO2005094803 A2
[3]. Thomas AA, De Meese J, Le Huerou Y, Non-charged thiamine analogs as inhibitors of enzyme transketolase. Bioorg Med Chem Lett. 2008 Jan 15;18(2):509-12.