invitrogen 17100-017 胶原酶I型

invitrogen 17100-017 胶原酶I型

规格

  • 1G
  • 产品详情
  • 产品参数

描述

I 型胶原酶从溶组织梭菌制备,用于细胞和组织的解离。

I 型胶原酶可应用于如下组织制备细胞:

• 上皮组织
•  肺
•  脂肪
• 肾上腺组织

胶原酶是一种蛋白酶,它能够特异性的结合 Pro-X-Glyc-Pro序列中,中性氨基酸(X)和甘氨酸之间的肽键。该序列高频率的存在于胶原中。胶原酶是唯一的一种可以降解广泛存在于结缔组织中的具有三股螺旋的天然胶原纤维的蛋白酶。

Life Technologies 大家庭中的细胞培养产品。
Life Technologies公司已准备就绪,以满足您的细胞培养需要一个全面的选择,包括培养基,营养添加剂,盐溶液,缓冲液,化学物质,解离试剂和转染的试剂。您可以依靠严格的质量控制和技术支持,以保证您的满意。仅供研究使用。 不用于任何动物或人类治疗或诊断使用。

原厂资料:

Collagenase is a protease that cleaves the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Gly-Pro, which is found with high frequency in collagen. Collagenase is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in connective tissues such as skin, tendon, blood vessels, and bone. Collagenase disaggregation is suitable for the culture of human tumors, mouse kidney, human adult and fetal brain, and many other tissues including epithelium. Collagenase is relatively gentle, dissociates well at physiological temperature and pH, and requires no mechanical agitation or special equipment.

Gibco® Collagenase Type I is isolated from Clostridium histolyticum and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation, and organ perfusions. Gibco® Collagenase Type I activity is guaranteed to be greater than 125 units/mg. Compared to other collagenase preparations, Gibco® Collagenase Type I has average levels of collagenase, caseinase, clostripain, and tryptic activities, and is well-suited for the digestion of fat, adrenal, and liver cells or tissues.

参数

英文名 Collagenase, Type I, powder
别名
品牌 Invitrogen产品订购
货号 17100017
货期 自助下单
单位
保存条件

invitrogen 17101-015 胶原酶II型

invitrogen 17101-015 胶原酶II型

规格

  • 1G
  • 产品详情
  • 产品参数

描述:

II 型胶原酶是从溶组织梭菌制备,用于细胞和组织的解离。

II 型胶原酶可应用于如下组织制备细胞:



甲状腺
心脏
唾液腺


胶原酶是一种蛋白酶,它能够特异性的结合 Pro-X-Glyc-Pro序列中,中性氨基酸(X)和甘氨酸之间的肽键。该序列高频率的存在于胶原中。胶原酶是唯一的一种可以降解广泛存在于结缔组织中的具有三股螺旋的天然胶原纤维的蛋白酶。

Collagenase is a protease that cleaves the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen. Collagenase is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in connective tissues such as skin, tendon, blood vessels, and bone. Collagenase disaggregation is suitable for the culture of human tumors, mouse kidney, human adult and fetal brain, and many other tissues including epithelium. Collagenase is relatively gentle, dissociates well at physiological temperature and pH, and requires no mechanical agitation or special equipment.

Gibco® Collagenase Type II is isolated from Clostridium histolyticum and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation and organ perfusions. Gibco® Collagenase Type II activity is guaranteed to be greater than 125 units/mg. Compared to other collagenase preparations, Gibco® Collagenase Type II has higher clostripain activity and is well-suited for the digestion of heart, bone, thyroid, cartilage, and liver tissues.

注意事项:

For Research Use Only. Not for use in diagnostic procedures.

参数

英文名 Collagenase, Type II, powder
别名
品牌 Invitrogen产品订购
货号 17101015
货期 1个现货其他1-2天货期
单位
保存条件

invitrogen 17104-019 胶原酶IV型

invitrogen 17104-019 胶原酶IV型

规格

  • 1G
  • 产品详情
  • 产品参数

描述

这种IV型胶原酶利用溶组织梭菌制备。适用于细胞和组织解离。

IV型胶原酶推荐用于胰腺组织和胰岛的分离。

胶原酶是一种蛋白酶,能够特异性地结合Pro-X-Glyc-Pro序列中中性氨基酸 (x) 和甘氨酸之间的肽键。该序列高频率地存在于胶原中。胶原酶是唯一一种可以降解广泛存在于结缔组织中具有三股螺旋的天然胶原纤维的蛋白酶。



Life Technologies细胞培养产品系列的一员


Life Technologies提供了种类齐全的试剂,包括培养基、添加剂、盐溶液、缓冲液、化学品、解离试剂和转染试剂,可满足您的细胞培养需要。您可以信赖我们严格的质量控制和技术支持,确保使您满意。

仅供研究使用。不得用于人类或动物的治疗诊断。

Collagenase is a protease that cleaves the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen. Collagenase is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in connective tissues such as skin, tendon, blood vessels, and bone. Collagenase disaggregation is suitable for the culture of human tumors, mouse kidney, human adult and fetal brain, and many other tissues including epithelium. Collagenase is relatively gentle, dissociates well at physiological temperature and pH, and requires no mechanical agitation or special equipment.

Gibco® Collagenase Type IV is isolated from Clostridium histolyticum and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation and organ perfusions. Gibco® Collagenase Type IV activity is guaranteed to be greater than 160 units/mg. Compared to other collagenase preparations, Gibco® Collagenase Type IV has low tryptic activity and is well-suited for the digestion of islet cells from the pancreas.

参数

英文名 Collagenase, Type IV, powder
别名
品牌 Invitrogen产品订购
货号 17104019
货期 少量现货
单位
保存条件 2-8℃

红细胞裂解液

红细胞裂解液

规格

  • 100ml
  • 500ml(拍3件发500ml备注就可以)
  • 产品详情
  • 产品参数

产品简介:

本产品是利用细胞内外存在盐离子浓度差而导致细胞膜胀破的原理来裂解无核红细胞的。    本产品经无菌处理,主要用于经酶消化分散的组织细胞的分离纯化,淋巴细胞的分离纯化以及组织细胞蛋白与核酸提取等实验中红细胞的去除。

使用说明:

1. 1倍体积的新鲜全血,加入3倍体积的红细胞裂解液。如1ml新鲜全血加入3ml红细胞裂解液,轻轻涡旋或颠倒混匀。

2. 冰上放置15分钟,其间轻轻涡旋混匀两次,红细胞裂解后,溶液应该是清亮透明的。

3. 收集细胞:4℃,450×g离心10分钟以沉淀白细胞,小心吸弃上清液。

4. 向白细胞沉淀中加入两倍体积的红细胞裂解液,轻轻涡旋充分重悬白细胞。如起始血液为1ml,则加入2ml的红细胞裂解液。

5. 4℃,450×g离心10分钟沉淀白细胞,小心并彻底吸去上清液。    6. 重悬细胞,用于后续实验;如提取RNA,最好于此步开始使用DEPC水配制的溶液进行操作。  (组织细胞处理:新鲜组织经胰酶或胶原酶等消化分散成单个细胞悬液,离心弃去上清液,其余同上。)

注意事项:

本裂解液为无菌产品,分离细胞用于细胞培养时请注意无菌操作。

为了您的安全与健康,请穿实验服并戴一次性手套操作。

说明书

点此下载说明书

参数

英文名 Red Blood Cell Lysis Buffer
别名
品牌 Jinpan
货号 C1074
货期
单位
保存条件