Cordycepin (3′-Deoxyadenosine) is a nucleoside derivative and inhibits IL-1β-induced MMP-1 and MMP-3 expression in rheumatoid arthritis synovial fibroblasts (RASFs) in a dose-dependent manner[1]. Cordycepin kills Mycobacterium tuberculosis through hijacking the bacterial adenosine kinase[2].
IC50 & Target[1]
MMP-1
MMP-3
体外研究 (In Vitro)
Cordycepin is a potent inhibitor of IL-1β-induced chemokine production and MMP expression and strongly blocks the p38/JNK/AP-1 signalling pathway in RASFs. The effect of Cordycepin on cellular toxicity of RASFs is assessed using MTT assay. Treatment of RASFs with Cordycepin (50 μM or 100 μM) for 24 h does not cause any significant change in cell viability. However, cell viability is slightly decreased when cells are incubated with 100 μM Cordycepin for 48 h[1].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量
251.24
Formula
C10H13N5O3
CAS 号
73-03-0
中文名称
虫草素;蛹虫草菌素;冬虫夏草菌素;3′-脱氧腺苷;虫草多糖
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-20°C
3 years
In solvent
-80°C
6 months
-20°C
1 month
溶解性数据
In Vitro:
DMSO : 50 mg/mL (199.01 mM; Need ultrasonic)
H2O : 2 mg/mL (7.96 mM; ultrasonic and warming and heat to 60°C)
[1]. Noh EM, et al. Cordycepin inhibits IL-1beta-induced MMP-1 and MMP-3 expression in rheumatoid arthritis synovial fibroblasts. Rheumatology (Oxford). 2009 Jan;48(1):45-8.
[2]. Huang F, et al. Cordycepin kills Mycobacterium tuberculosis through hijacking the bacterial adenosine kinase. PLoS One. 2019 Jun 14;14(6):e0218449.
Cell Assay [1]
RASFs (2×104 cells/well) are treated with various concentrations of Cordycepin (50 μM or 100 μM). After incubation for 1 h, 12 h and 24 h, cells are washed twice with PBS, MTT (0.5 mg/mL PBS) is added to each well and incubated at 37°C for 30 min. Formazan crystals formed are dissolved by adding DMSO (100 μL/well) and the absorbance is read at 570 nm using a microplate reader[1].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献
[1]. Noh EM, et al. Cordycepin inhibits IL-1beta-induced MMP-1 and MMP-3 expression in rheumatoid arthritis synovial fibroblasts. Rheumatology (Oxford). 2009 Jan;48(1):45-8.
[2]. Huang F, et al. Cordycepin kills Mycobacterium tuberculosis through hijacking the bacterial adenosine kinase. PLoS One. 2019 Jun 14;14(6):e0218449.
5′-Methylthioadenosine (5′-(Methylthio)-5′-deoxyadenosine) is a nucleoside generated from S-adenosylmethionine (SAM) during polyamine synthesis[1]. 5′-Methylthioadenosine suppresses tumors by inhibiting tumor cell proliferation, invasion, and the induction of apoptosis while controlling the inflammatory micro-environments of tumor tissue. 5′-Methylthioadenosine and its associated materials have striking regulatory effects on tumorigenesis[2].
IC50 & Target
Human Endogenous Metabolite
Microbial Metabolite
体外研究 (In Vitro)
5′-Methylthioadenosine protects MTAP+ cells significantly better than MTAP− cells from 6TG (6′-thioguanine) toxicity[3].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[3]
Cell Line:
MTAP+ and MTAP− NIH3T3 cells, isogenic MTAP+ and MTAP− cell line derived from a human HT1080 fibrosarcoma cell line
Concentration:
6TG and 2FA in combination with 10 μM MTA
Incubation Time:
48 hours
Result:
Significantly increased IC50 concentration in MTAP+ cells, but no significant change in MTAP− cells.
体内研究 (In Vivo)
The 2FA (2′-fluoroadenine) + MTA (5′-Methylthioadenosine) combination (100 mg/kg MTA; 20 mg/kg 2FA) inhibits tumor growth of four different MTAP− human tumor cell lines in mouse xenograft models[3].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
297.33
Formula
C11H15N5O3S
CAS 号
2457-80-9
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Tang Y, et al. 5′-Methylthioadenosine attenuates ischemia reperfusion injury after liver transplantation in rats. Inflammation. 2014;37(5):1366-1373.
[2]. Li Y, et al. 5′-Methylthioadenosine and Cancer: old molecules, new understanding. J Cancer. 2019;10(4):927-936.
[3]. Tang B, et al. Specific Targeting of MTAP-Deleted Tumors with a Combination of 2′-Fluoroadenine and 5′-Methylthioadenosine. Cancer Res. 2018;78(15):4386-4395.