标签归档：pka

Malantide

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Malantide  纯度: 98.56%

Malantide 是一种合成的十二肽，由被磷酸化酶激酶β亚基上的 PKA 磷酸化的位点衍生而来。Malantide 是 PKA 的高度特异性底物，Km 为 15 μM，并且在各种大鼠组织提取物中显示出 PKI 抑制 > 90％底物磷酸化。Malantide 也是 PKC 的有效底物，Km 为 16 μM。

Malantide

Malantide Chemical Structure

CAS No. : 86555-35-3

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5 mg ￥1800 In-stock
10 mg ￥2800 In-stock
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Malantide 相关产品

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生物活性

Malantide is a synthetic dodecapeptide derived from the site phosphorylated by cAMP-dependent protein kinase (PKA) on the β-subunit of phosphorylase kinase. Malantide is a highly specific substrate for PKA with a Km of 15 μM and shows protein inhibitor (PKI) inhibition >90% substrate phosphorylation in various rat tissue extracts[1]. Malantide is also an efficient substrate for PKC with a Km of 16 μM[2].

IC50 & Target

Km: 15 μM (PKA)[1], 16 μM (PKC)[2], 233 μM (PKG)[1]
体外研究
(In Vitro)

The Km values of Malantide are 15 μM and 223 μM for PKA and PKG, respectively. The Vmax values are 23.8 units/mg and 6.6 units/mg for for PKA and PKG, respectively[1].
A statistically significant effect of isoprenaline on the activity ratio of guinea-pig heart was only: observed with Malantide[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1633.89

Formula

C72H124N22O21
CAS 号

86555-35-3
Sequence

Arg-Thr-Lys-Arg-Ser-Gly-Ser-Val-Tyr-Glu-Pro-Leu-Lys-Ile
Sequence Shortening

RTKRSGSVYEPLKI
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80°C 2 years
-20°C 1 year
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Murray KJ, et al. Use of a synthetic dodecapeptide (malantide) to measure the cyclic AMP-dependent protein kinase activity ratio in a variety of tissues. Biochem J. 1990 May 1;267(3):703-8.

    [2]. Z H Zhao, et al. Characterization of a New Substrate for Protein Kinase C: Assay by Continuous Fluorometric Monitoring and High Performance Liquid Chromatography. Biochem Biophys Res Commun. 1991 May 15;176(3):1454-61.

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多肽定制PKA Inhibitor Substrate 编码

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上海金畔生物科技有限公司可以定制不同序列多肽，可以访问官网了解更多产品信息。

名称 PKA Inhibitor Substrate
编码
别名 PKA Inhibitor Substrate
纯度 80%，90%，95%，98%，99%
重量 1mg,5mg,10mg,50mg,100mg,1g
序列（单字母缩写） GRTGRRNPSIHDIL
序列（三字母缩写） Gly-Arg-Thr-Gly-Arg-Arg-Asn-pSer-Ile-His-Asp-Ile-Leu
基本描述
溶解度
分子量 1574.69
化学式 C61H108N25O22P
存储条件 Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents PKA Inhibitor Substrate 编码
Figures PKA Inhibitor Substrate 编码
Reference
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多肽定制PKA Regulatory Subunit II Substrate 编码

发表于由

上海金畔生物科技有限公司可以定制不同序列多肽，可以访问官网了解更多产品信息。

名称 PKA Regulatory Subunit II Substrate
编码
别名 PKA Regulatory Subunit II Substrate
纯度 80%，90%，95%，98%，99%
重量 1mg,5mg,10mg,50mg,100mg,1g
序列（单字母缩写） DLDVPIPGRFDRRVPSVAAE
序列（三字母缩写） Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-pSer-Val-Ala-Ala-Glu
基本描述
溶解度
分子量 2192.39
化学式 C92H151N28O32P
存储条件 Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents PKA Regulatory Subunit II Substrate 编码
Figures PKA Regulatory Subunit II Substrate 编码
Reference
C端
N端
化学桥