Baohuoside I 是从朝鲜淫羊藿中得到的黄酮类化合物,作为 CXCR4 的抑制剂,能够抑制 CXCR4 的表达,诱导凋亡,具有抗肿瘤活性。
Baohuoside I Chemical Structure
CAS No. : 113558-15-9
规格
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数量
10 mM * 1 mL in DMSO
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生物活性
Baohuoside I, a flavonoid isolated from Epimedium koreanum Nakai, acts as an inhibitor of CXCR4, downregulates CXCR4 expression, induces apoptosis and shows anti-tumor activity.
IC50 & Target
CXCR4
体外研究 (In Vitro)
Baohuoside I is an inhibitor of CXCR4, and downregulates CXCR4 expression at 12-25 μM. Baohuoside I (0-25 μM) suppresses NF-κB activation in a dose-dependent manner, suppresses CXCL12 induced the invasion of cervical cancer cells. Baohuoside I also inhibits invasion of breast cancer cells[1]. Baohuoside I inhibits A549 cell viability, with IC50s of 25.1 μM at 24 h, 11.5 μM and 9.6 μM at 48 h and 72 h, respectively. Baohuoside I ((25 μM) suppresses the caspase cascade in A549 cells, elevates ROS levels and activates JNK and p38MAPK signaling cascade[2]. Baohuoside I (3.125, 6.25, 12.5, 25.0 and 50.0 µg/mL) significantly and dose-dependently blocks the growth of esophageal squamous cell carcinoma Eca109 cells, with an IC50 of 4.8 µg/mL at 48 h[3].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究 (In Vivo)
Baohuoside I (25 mg/kg) decreases β-catenin protein levels, cyclin D1 and survivin expression in nude mice[3].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
514.52
Formula
C27H30O10
CAS 号
113558-15-9
中文名称
宝藿苷 I;淫羊藿次苷 Ⅱ;宝霍苷 I
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Kim B, et al. Baohuoside I suppresses invasion of cervical and breast cancer cells through the downregulation of CXCR4 chemokine receptor expression. Biochemistry. 2014 Dec 9;53(48):7562-9.
[2]. Song J, et al. Reactive oxygen species-mediated mitochondrial pathway is involved in Baohuoside I-induced apoptosis in human non-small cell lung cancer. Chem Biol Interact. 2012 Jul 30;199(1):9-17.
[3]. Wang L, et al. The flavonoid Baohuoside-I inhibits cell growth and downregulates survivin and cyclin D1 expression in esophageal carcinoma via β-catenin-dependent signaling. Oncol Rep. 2011 Nov;26(5):1149-56.
Cell Assay [2]
The cytotoxicity effect of Baohuoside I on A549 cells is determined by MTT assay. Cells (1×104 cells/well) are seeded in a 96-well plate, and treated with Baohuoside I (6.25, 12.5, and 25 μM) or 1 mM NAC for 24, 48 or 72 h. After MTT containing medium is removed, the crystals that have formed are dissolved by the addition of DMSO to each well. After mixing, the absorbance of the cells is measured at 540 nm by using Multiskan Spectrum Microplate Reader[2].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration [3]
Mice[3] Female Balb/c nude mice (4- to 6-weeks-old) are used in the assay. Subconfluent Eca109-Luc cells are harvested and resuspended in PBS to a final density of 2 × 107 cells/mL. Prior to injection, cells are resuspended in PBS and analyzed by 0.4% trypan blue exclusion assay (viable cells >90%). For subcutaneous injection, 1 × 107 Eca109-Luc cells in 200 µL PBS are injected into the left flank of each mouse using 27G needles. At 1 week after tumor cell injection, Baohuoside I (25 mg/kg per mouse) is injected intralesionally once a day, whereas the 10 mice intended for vehicle treatment are administered an equal volume of PBS[3].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献
[1]. Kim B, et al. Baohuoside I suppresses invasion of cervical and breast cancer cells through the downregulation of CXCR4 chemokine receptor expression. Biochemistry. 2014 Dec 9;53(48):7562-9.
[2]. Song J, et al. Reactive oxygen species-mediated mitochondrial pathway is involved in Baohuoside I-induced apoptosis in human non-small cell lung cancer. Chem Biol Interact. 2012 Jul 30;199(1):9-17.
[3]. Wang L, et al. The flavonoid Baohuoside-I inhibits cell growth and downregulates survivin and cyclin D1 expression in esophageal carcinoma via β-catenin-dependent signaling. Oncol Rep. 2011 Nov;26(5):1149-56.