DBCO PEG Folic acid, Folate PEG DBCO Cat. No. PG2-DBFA-5k 5000 Da 5 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DBCO PEG Folic acid, Folate PEG DBCO

Cat. No. PG2-DBFA-5k
Specification 5000 Da
Unit Size 5 mg
Price $385.00

Qty Add to Cart

Synonym: Folic acid PEG DBCO, Folate PEG DBCO, DBCO PEG folate, DBCO PEG Folic acid.

Description:

DBCO PEG Folic acid, also called DBCO PEG folate, is one of Nanocs' folic acid PEG derivatives that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG folate derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. Folic acid, is also known as vitamin M, vitamin B9 or pteroyl-L-glutamic acid. Folic acid is an essential bioactive molecule for numerous biological functions. It participates in the synthesis, repairing and methylation of DNA as well as to act as a cofactor in many biological reactions. DBCO functional folic acid can be easily attached to azide functionalized molecules, particles or solid surfaces.

Physical Properties:

  • Yellow solid;
  • Soluble in DMSO;
  • Reactive group: DBCO;
  • Reactive to: Azide;
  • Applications: Click chemistry;
  • Purity: >95%;

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction solution.
  • Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

  • Store at -20 0C. Desicate. Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

mPEG-DBCO, PEG Dibenzylcyclooctyne Cat. No. PG1-DB-5k 5000 Da 25 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

mPEG-DBCO, PEG Dibenzylcyclooctyne

Cat. No. PG1-DB-5k mPEG-DBCO, PEG Dibenzylcyclooctyne           Cat. No. PG1-DB-5k     5000 Da    25 mg
Specification 5000 Da
Unit Size 25 mg
Price $385.00

Qty Add to Cart

Synonym: DBCO PEG, mPEG DBCO, Methoxy PEG DBCO, Polyethylene glycol DBCO

Description:

mPEG DBCO, also called methoxy PEG DBCO, is a pegylated DBCO (dibenzocyclooctyne) derivative that can react with azide group without need any metal catalyst. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free Click reaction, is a bioorthogaonal reaction that enables the conjugation of two molecules in aqueous solution. PEG DBCO derivatives have excellent water solubility, possess fast kinetics and stability in aqueous buffer. They are one of the best pegylation reagents to modify proteins, peptides, antibodies, biopolymers and particles via copper free Click Chemistry reaction.

Product Specifications:

  • Form: Solid
  • Color: Yellow to light yellow
  • Solubility: Soluble in water
  • Reactive group: DBCO (Dibenzooctyne)
  • Reactive to: Azide group (-N3)
  • Purity: >95%

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add PEG DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage:

  • Store at -20 0C; Desiccate; Avoid frequent thaw and freeze.

References:

Click here to see hundred of publications using Nanocs PEGs.

Documents
  • SDS
  • DataSheet

Related Products
PG1-DB-5k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-2k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-10k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-20k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-30k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-40k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-1k , mPEG-DBCO, PEG Dibenzylcyclooctyne

Tags: DBCO PEG,

DBCO PEG NHS Cat. No. PG2-DBNS-20k 20000 Da 20 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DBCO PEG NHS

Cat. No. PG2-DBNS-20k DBCO PEG NHS           Cat. No. PG2-DBNS-20k     20000 Da    20 mg
Specification 20000 Da
Unit Size 20 mg
Price $485.00

Qty Add to Cart

Description:

DBCO PEG NHS is one of Nanocs’ amine reactive PEG derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO (Dibenzylcyclooctyne) PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.

Physical Properties:

  • Off-white or light yellow solid;
  • Soluble in regular aqeous solution;
  • Functional group: DBCO;
  • Reactive toward: Azide (-N3):
  • PEG MW: 20000 Da;

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

  • Store at -20 0C.
Documents
  • SDS
  • DataSheet

mPEG-DBCO, PEG Dibenzylcyclooctyne Cat. No. PG1-DB-2k 2000 Da 25 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

mPEG-DBCO, PEG Dibenzylcyclooctyne

Cat. No. PG1-DB-2k mPEG-DBCO, PEG Dibenzylcyclooctyne           Cat. No. PG1-DB-2k     2000 Da    25 mg
Specification 2000 Da
Unit Size 25 mg
Price $425.00

Qty Add to Cart

Synonym: DBCO PEG, mPEG DBCO, Methoxy PEG DBCO, Polyethylene glycol DBCO

Description:

mPEG DBCO, also called methoxy PEG DBCO, is a pegylated DBCO (dibenzocyclooctyne) derivative that can react with azide group without need any metal catalyst. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free Click reaction, is a bioorthogaonal reaction that enables the conjugation of two molecules in aqueous solution. PEG DBCO derivatives have excellent water solubility, possess fast kinetics and stability in aqueous buffer. They are one of the best pegylation reagents to modify proteins, peptides, antibodies, biopolymers and particles via copper free Click Chemistry reaction.

Product Specifications:

  • Form: Solid
  • Color: Yellow to light yellow
  • Solubility: Soluble in water
  • Reactive group: DBCO (Dibenzooctyne)
  • Reactive to: Azide group (-N3)
  • Purity: >95%

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add PEG DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage:

  • Store at -20 0C; Desiccate; Avoid frequent thaw and freeze.

References:

Click here to see hundred of publications using Nanocs PEGs.

Documents
  • SDS
  • DataSheet

Related Products
PG1-DB-5k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-2k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-10k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-20k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-30k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-40k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-1k , mPEG-DBCO, PEG Dibenzylcyclooctyne

Tags: DBCO PEG,

DBCO PEG NHS Cat. No. PG2-DBNS-30k 30000 Da 20 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DBCO PEG NHS

Cat. No. PG2-DBNS-30k DBCO PEG NHS           Cat. No. PG2-DBNS-30k     30000 Da    20 mg
Specification 30000 Da
Unit Size 20 mg
Price $485.00

Qty Add to Cart

Description:

DBCO PEG NHS is one of Nanocs’ amine reactive PEG derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO (Dibenzylcyclooctyne) PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.

Physical Properties:

  • Off-white or light yellow solid;
  • Soluble in regular aqeous solution;
  • Functional group: DBCO;
  • Reactive toward: Azide (-N3):
  • PEG MW: 30000 Da;

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

  • Store at -20 0C.
Documents
  • SDS
  • DataSheet

mPEG-DBCO, PEG Dibenzylcyclooctyne Cat. No. PG1-DB-10k 10000 Da 25 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

mPEG-DBCO, PEG Dibenzylcyclooctyne

Cat. No. PG1-DB-10k mPEG-DBCO, PEG Dibenzylcyclooctyne           Cat. No. PG1-DB-10k     10000 Da    25 mg
Specification 10000 Da
Unit Size 25 mg
Price $485.00

Qty Add to Cart

Synonym: DBCO PEG, mPEG DBCO, Methoxy PEG DBCO, Polyethylene glycol DBCO

Description:

mPEG DBCO, also called methoxy PEG DBCO, is a pegylated DBCO (dibenzocyclooctyne) derivative that can react with azide group without need any metal catalyst. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free Click reaction, is a bioorthogaonal reaction that enables the conjugation of two molecules in aqueous solution. PEG DBCO derivatives have excellent water solubility, possess fast kinetics and stability in aqueous buffer. They are one of the best pegylation reagents to modify proteins, peptides, antibodies, biopolymers and particles via copper free Click Chemistry reaction.

Product Specifications:

  • Form: Solid
  • Color: Yellow to light yellow
  • Solubility: Soluble in water
  • Reactive group: DBCO (Dibenzooctyne)
  • Reactive to: Azide group (-N3)
  • Purity: >95%

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add PEG DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage:

  • Store at -20 0C; Desiccate; Avoid frequent thaw and freeze.

References:

Click here to see hundred of publications using Nanocs PEGs.

Documents
  • SDS
  • DataSheet

Related Products
PG1-DB-5k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-2k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-10k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-20k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-30k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-40k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-1k , mPEG-DBCO, PEG Dibenzylcyclooctyne

Tags: DBCO PEG,

DBCO-NHS ester 2

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

DBCO-NHS ester 2 

DBCO-NHS ester 2 是一种用于制备抗体偶联药物 (ADC) 的可降解 linker,是一种二苯基环辛酮 (DBCO) 衍生物,用于无铜 click 反应。

DBCO-NHS ester 2

DBCO-NHS ester 2 Chemical Structure

CAS No. : 1384870-47-6

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

DBCO-NHS ester 2 is a cleavable linker that is used for making antibody-drug conjugate (ADC). DBCO-NHS ester 2 is a derivative of Dibenzylcyclooctyne (DBCO) used in copper-free click chemistry[1].

分子量

430.45

Formula

C25H22N2O5

CAS 号

1384870-47-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

溶解性数据
In Vitro: 

DMSO : 14.29 mg/mL (33.20 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3232 mL 11.6158 mL 23.2315 mL
5 mM 0.4646 mL 2.3232 mL 4.6463 mL
10 mM 0.2323 mL 1.1616 mL 2.3232 mL

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.43 mg/mL (3.32 mM); Clear solution

    此方案可获得 ≥ 1.43 mg/mL (3.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 14.3 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献
  • [1]. Tang F, et al. Chemoenzymatic synthesis of glycoengineered IgG antibodies and glycosite-specific antibody-drug conjugates. Nat Protoc. 2017 Aug;12(8):1702-1721.

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

DBCO PEG Folic acid Cat. No. PG2-DBFA-2k-1 2000 Da 5 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DBCO PEG Folic acid

Cat. No. PG2-DBFA-2k-1
Specification 2000 Da
Unit Size 5 mg
Price $385.00

Qty Add to Cart

Description:

DBCO PEG Folic acid, also called DBCO PEG folate, is one of Nanocs’ folic acid PEG derivatives that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG folate derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. Folic acid, is also known as vitamin M, vitamin B9 or pteroyl-L-glutamic acid. Folic acid is an essential bioactive molecule for numerous biological functions. It participates in the synthesis, repairing and methylation of DNA as well as to act as a cofactor in many biological reactions. DBCO functional folic acid can be easily attached to azide functionalized molecules, particles or solid surfaces.

Physical Properties:

  • Yellow solid;
  • Soluble in DMSO;

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction solution.
  • Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

  • Store at -20 0C. Desicate. Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

mPEG-DBCO, PEG Dibenzylcyclooctyne Cat. No. PG1-DB-20k 20000 Da 25 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

mPEG-DBCO, PEG Dibenzylcyclooctyne

Cat. No. PG1-DB-20k mPEG-DBCO, PEG Dibenzylcyclooctyne           Cat. No. PG1-DB-20k     20000 Da    25 mg
Specification 20000 Da
Unit Size 25 mg
Price $485.00

Qty Add to Cart

Methoxy PEG DBCO, MW 550, 750, 1000, 2000, 3400, 5000, 10k, 20k, 30k, 40k

Description:

mPEG DBCO, also called methoxy PEG DBCO, is a pegylated DBCO (dibenzocyclooctyne) derivative that can react with azide group without need any metal catalyst. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free Click reaction, is a bioorthogaonal reaction that enables the conjugation of two molecules in aqueous solution. PEG DBCO derivatives have excellent water solubility, possess fast kinetics and stability in aqueous buffer. They are one of the best pegylation reagents to modify proteins, peptides, antibodies, biopolymers and particles via copper free Click Chemistry reaction.

Product Specifications:

  • Appearance: Light yellow to white solid, semi-solid depends on molecular weight of PEG;
  • Solubility: Soluble in water, ethanol, DMSO, etc.;
  • Reactivity: DBCO reacts with azide group (-N3).

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add PEG DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage:

  • Store at -20 0C; Desiccate; Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Related Products
PG1-DB-5k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-2k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-10k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-1k , mPEG-DBCO, PEG Dibenzylcyclooctyne

Tags: DBCO PEG,

DBCO-PEG4-NHS ester

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

DBCO-PEG4-NHS ester  纯度: 97.97%

DBCO-PEG4-NHS ester 是一种 PROTAC linker,属于 PEG 类。可用于合成 PROTAC 分子。

DBCO-PEG4-NHS ester

DBCO-PEG4-NHS ester Chemical Structure

CAS No. : 1427004-19-0

规格 价格 是否有货 数量
5 mg ¥1800 In-stock
10 mg ¥3000 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

生物活性

DBCO-PEG4-NHS ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1].

IC50 & Target

PEGs

 

体外研究
(In Vitro)

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

649.69

Formula

C34H39N3O10

CAS 号

1427004-19-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

溶解性数据
In Vitro: 

DMSO : 83.33 mg/mL (128.26 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.5392 mL 7.6960 mL 15.3920 mL
5 mM 0.3078 mL 1.5392 mL 3.0784 mL
10 mM 0.1539 mL 0.7696 mL 1.5392 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (3.20 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.20 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.08 mg/mL (3.20 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.08 mg/mL (3.20 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (3.20 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.20 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献
  • [1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562

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DBCO PEG Folic acid Cat. No. PG2-DBFA-1k 1000 Da 5 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DBCO PEG Folic acid

Cat. No. PG2-DBFA-1k
Specification 1000 Da
Unit Size 5 mg
Price $385.00

Qty Add to Cart

Description:

DBCO PEG Folic acid, also called DBCO PEG folate, is one of Nanocs’ folic acid PEG derivatives that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG folate derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. Folic acid, is also known as vitamin M, vitamin B9 or pteroyl-L-glutamic acid. Folic acid is an essential bioactive molecule for numerous biological functions. It participates in the synthesis, repairing and methylation of DNA as well as to act as a cofactor in many biological reactions. DBCO functional folic acid can be easily attached to azide functionalized molecules, particles or solid surfaces.

Physical Properties:

  • Yellow solid;
  • Soluble in DMSO;

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction solution.
  • Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

  • Store at -20 0C. Desicate. Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

mPEG-DBCO, PEG Dibenzylcyclooctyne Cat. No. PG1-DB-30k 30000 Da 25 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

mPEG-DBCO, PEG Dibenzylcyclooctyne

Cat. No. PG1-DB-30k mPEG-DBCO, PEG Dibenzylcyclooctyne           Cat. No. PG1-DB-30k     30000 Da    25 mg
Specification 30000 Da
Unit Size 25 mg
Price $485.00

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Methoxy PEG DBCO, MW 550, 750, 1000, 2000, 3400, 5000, 10k, 20k, 30k, 40k

Description:

mPEG DBCO, also called methoxy PEG DBCO, is a pegylated DBCO (dibenzocyclooctyne) derivative that can react with azide group without need any metal catalyst. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free Click reaction, is a bioorthogaonal reaction that enables the conjugation of two molecules in aqueous solution. PEG DBCO derivatives have excellent water solubility, possess fast kinetics and stability in aqueous buffer. They are one of the best pegylation reagents to modify proteins, peptides, antibodies, biopolymers and particles via copper free Click Chemistry reaction.

Product Specifications:

  • Appearance: Light yellow to white solid, semi-solid depends on molecular weight of PEG;
  • Solubility: Soluble in water, ethanol, DMSO, etc.;
  • Reactivity: DBCO reacts with azide group (-N3).

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add PEG DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage:

  • Store at -20 0C; Desiccate; Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Related Products
PG1-DB-5k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-2k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-10k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-1k , mPEG-DBCO, PEG Dibenzylcyclooctyne

Tags: DBCO PEG,

DBCO PEG Folic acid Cat. No. PG2-DBFA-10k 10000 Da 5 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DBCO PEG Folic acid

Cat. No. PG2-DBFA-10k
Specification 10000 Da
Unit Size 5 mg
Price $385.00

Qty Add to Cart

Description:

DBCO PEG Folic acid, also called DBCO PEG folate, is one of Nanocs’ folic acid PEG derivatives that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG folate derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. Folic acid, is also known as vitamin M, vitamin B9 or pteroyl-L-glutamic acid. Folic acid is an essential bioactive molecule for numerous biological functions. It participates in the synthesis, repairing and methylation of DNA as well as to act as a cofactor in many biological reactions. DBCO functional folic acid can be easily attached to azide functionalized molecules, particles or solid surfaces.

Physical Properties:

  • Yellow solid;
  • Soluble in DMSO;

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction solution.
  • Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

  • Store at -20 0C. Desicate. Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

mPEG-DBCO, PEG Dibenzylcyclooctyne Cat. No. PG1-DB-40k 40000 Da 25 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

mPEG-DBCO, PEG Dibenzylcyclooctyne

Cat. No. PG1-DB-40k mPEG-DBCO, PEG Dibenzylcyclooctyne           Cat. No. PG1-DB-40k     40000 Da    25 mg
Specification 40000 Da
Unit Size 25 mg
Price $485.00

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Methoxy PEG DBCO, MW 550, 750, 1000, 2000, 3400, 5000, 10k, 20k, 30k, 40k

Description:

mPEG DBCO, also called methoxy PEG DBCO, is a pegylated DBCO (dibenzocyclooctyne) derivative that can react with azide group without need any metal catalyst. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free Click reaction, is a bioorthogaonal reaction that enables the conjugation of two molecules in aqueous solution. PEG DBCO derivatives have excellent water solubility, possess fast kinetics and stability in aqueous buffer. They are one of the best pegylation reagents to modify proteins, peptides, antibodies, biopolymers and particles via copper free Click Chemistry reaction.

Product Specifications:

  • Appearance: Light yellow to white solid, semi-solid depends on molecular weight of PEG;
  • Solubility: Soluble in water, ethanol, DMSO, etc.;
  • Reactivity: DBCO reacts with azide group (-N3).

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add PEG DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage:

  • Store at -20 0C; Desiccate; Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Related Products
PG1-DB-5k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-2k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-10k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-1k , mPEG-DBCO, PEG Dibenzylcyclooctyne

Tags: DBCO PEG,

DBCO PEG Folic acid Cat. No. PG2-DBFA-20k 20000 Da 20 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DBCO PEG Folic acid

Cat. No. PG2-DBFA-20k
Specification 20000 Da
Unit Size 20 mg
Price $485.00

Qty Add to Cart

Description:

DBCO PEG Folic acid, also called DBCO PEG folate, is one of Nanocs’ folic acid PEG derivatives that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG folate derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. Folic acid, is also known as vitamin M, vitamin B9 or pteroyl-L-glutamic acid. Folic acid is an essential bioactive molecule for numerous biological functions. It participates in the synthesis, repairing and methylation of DNA as well as to act as a cofactor in many biological reactions. DBCO functional folic acid can be easily attached to azide functionalized molecules, particles or solid surfaces.

Physical Properties:

  • Yellow solid;
  • Soluble in DMSO;

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction solution.
  • Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

  • Store at -20 0C. Desiccate. Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

mPEG-DBCO, PEG Dibenzylcyclooctyne Cat. No. PG1-DB-1k 1000 Da 25 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

mPEG-DBCO, PEG Dibenzylcyclooctyne

Cat. No. PG1-DB-1k mPEG-DBCO, PEG Dibenzylcyclooctyne           Cat. No. PG1-DB-1k     1000 Da    25 mg
Specification 1000 Da
Unit Size 25 mg
Price $425.00

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Synonym: DBCO PEG, mPEG DBCO, Methoxy PEG DBCO, Polyethylene glycol DBCO

Description:

mPEG DBCO, also called methoxy PEG DBCO, is a pegylated DBCO (dibenzocyclooctyne) derivative that can react with azide group without need any metal catalyst. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free Click reaction, is a bioorthogaonal reaction that enables the conjugation of two molecules in aqueous solution. PEG DBCO derivatives have excellent water solubility, possess fast kinetics and stability in aqueous buffer. They are one of the best pegylation reagents to modify proteins, peptides, antibodies, biopolymers and particles via copper free Click Chemistry reaction.

Product Specifications:

  • Form: Solid
  • Color: Yellow to light yellow
  • Solubility: Soluble in water
  • Reactive group: DBCO (Dibenzooctyne)
  • Reactive to: Azide group (-N3)
  • Purity: >95%

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add PEG DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage:

  • Store at -20 0C; Desiccate; Avoid frequent thaw and freeze.

References:

Click here to see hundred of publications using Nanocs PEGs.

Documents
  • SDS
  • DataSheet

Related Products
PG1-DB-5k , mPEG-DBCO, PEG Dibenzylcyclooctyne
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PG1-DB-10k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-20k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-30k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-40k , mPEG-DBCO, PEG Dibenzylcyclooctyne
PG1-DB-1k , mPEG-DBCO, PEG Dibenzylcyclooctyne

Tags: DBCO PEG,

DBCO-PEG4-Biotin

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

DBCO-PEG4-Biotin  纯度: 98.18%

DBCO-PEG4-Biotin 是一种含生物素基团和 4 个 PEG 的 azadibenzocyclooctyne-biotin 衍生物。DBCO-PEG4-Biotin 是一种用途广泛的生物素酰化试剂,能通过无铜催化烷基叠氮化反应 (SPAAC) 将生物素的一部分引入叠氮标记的生物分子中。

DBCO-PEG4-Biotin

DBCO-PEG4-Biotin Chemical Structure

CAS No. : 1255942-07-4

规格 价格 是否有货 数量
5 mg ¥3150 In-stock
10 mg   询价  
50 mg   询价  

* Please select Quantity before adding items.

DBCO-PEG4-Biotin 相关产品

相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Cancer Compound Library

生物活性

DBCO-PEG4-Biotin is an azadibenzocyclooctyne-biotin derivative containing a biotin group and 4 PEGs. DBCO-PEG4-Biotin is a versatile biotinylation reagent used for the introduction of a biotin moiety to azide-labeled biomolecules via copper-free strain-promoted alkyne-azide click chemistry (SPAAC) reaction[1].

体外研究
(In Vitro)

The alkyne group can react with azide moiety in copper-free Click Chemistry reaction to form a stable triazole linkage.

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

749.92

Formula

C39H51N5O8S

CAS 号

1255942-07-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 100 mg/mL (133.35 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.3335 mL 6.6674 mL 13.3348 mL
5 mM 0.2667 mL 1.3335 mL 2.6670 mL
10 mM 0.1333 mL 0.6667 mL 1.3335 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (3.33 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.33 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.33 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.33 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (3.33 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.33 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献
  • [1]. Hammink R, et al. Affinity-Based Purification of Polyisocyanopeptide Bioconjugates. Bioconjug Chem. 2017 Oct 18;28(10):2560-2568.

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

DBCO-NHS ester 3

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

DBCO-NHS ester 3 

DBCO-NHS ester 3 (Compound 12) 是一种用于制备抗体偶联药物 (ADC) 的可降解 linker。DBCO-NHS ester 3 是一种二苯基环辛酮 (DBCO) 衍生物,通过 MeOND 和 DIBAC 的羧酸部分被 N-羟基琥珀酰亚胺活化而获得的。

DBCO-NHS ester 3

DBCO-NHS ester 3 Chemical Structure

CAS No. : 1393350-27-0

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

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生物活性

DBCO-NHS ester 3 (Compound 12) is a cleavable linker that is used for making antibody-drug conjugate (ADC). DBCO-NHS ester 3 is a derivative of Dibenzylcyclooctyne (DBCO) obtained by activation of N-hydroxysuccinimide by the carboxylic acid moiety of both methyl-oxanorbornadiene (MeOND) and dibenzoazacyclooctyne (DIBAC)[1][2].

IC50 & Target[1]

Cleavable

 

分子量

416.43

Formula

C24H20N2O5

CAS 号

1393350-27-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Silvie A. Meeuwissen, et al. Copper-free click chemistry on polymersomes: pre- vs. post-self-assembly functionalization. Polymer Chemistry. 2012, 3: 1783-1795.

    [2]. Tang F, et al. Chemoenzymatic synthesis of glycoengineered IgG antibodies and glycosite-specific antibody-drug conjugates. Nat Protoc. 2017 Aug;12(8):1702-1721.

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

DBCO-(PEG2-Val-Cit-PAB)2

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

DBCO-(PEG2-Val-Cit-PAB)2 

DBCO-(PEG2-Val-Cit-PAB)2 是一种可降解 (cleavable) 的含 4 个单元 PEG 的 ADC linker,可用于合成抗体偶联药物 (ADC)。DBCO-(PEG2-Val-Cit-PAB)2 也是一种基于 PEG 的 PROTAC linker,可用于 PROTAC 的合成。

DBCO-(PEG2-Val-Cit-PAB)2

DBCO-(PEG2-Val-Cit-PAB)2 Chemical Structure

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

DBCO-(PEG2-Val-Cit-PAB)2 is a dual cleavable ADC linker for antibody-drug conjugates (ADCs). DBCO-(PEG2-Val-Cit-PAB)2 is also a PEG-based PROTAC linker that can be used in the synthesis of PROTACs.

IC50 & Target

Cleavable

 

Alkyl/ether

 

PEGs

 

体外研究
(In Vitro)

ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker.
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1347.56

Formula

C69H94N12O16

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

DBCO PEG Maleimide, DBCO-PEG-Mal Cat. No. PG2-DBML-1k 1000 Da 20 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DBCO PEG Maleimide, DBCO-PEG-Mal

Cat. No. PG2-DBML-1k DBCO PEG Maleimide, DBCO-PEG-Mal           Cat. No. PG2-DBML-1k     1000 Da    20 mg
Specification 1000 Da
Unit Size 20 mg
Price $485.00

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Description:

Maleimide PEG DBCO (dibenzocycolctyne) is one of Nanocs DBCO PEG derivatives that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. Meanwhile, maleimide group can react with sulfhydryl (Thoil) groups at pH 6.5-7.5 easily. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.

Physical Properties:

  • White/off-white solid or viscous liquid depends on molecule weight;
  • Soluble in aqeous buffer and DMSO;

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

  • Store at -20 0C. Desiccate. Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet