海门其林贝尔台式冷冻恒温振荡器THZ-C-1(已停产)
振荡频率:30-280次/分(数显)
振幅:旋转30mm
最大容量:1000ml*6 250ml*20
定时范围:0-999分
恒温范围:5–60℃(室温25℃时)
恒温精度:0.2℃
温度均匀度:±0.5℃
输入功率:600W
外型尺寸:800*570*500mm
重量:56KG
电源:220V 50Hz
海门其林贝尔台式冷冻恒温振荡器THZ-C-1(已停产)
振荡频率:30-280次/分(数显)
振幅:旋转30mm
最大容量:1000ml*6 250ml*20
定时范围:0-999分
恒温范围:5–60℃(室温25℃时)
恒温精度:0.2℃
温度均匀度:±0.5℃
输入功率:600W
外型尺寸:800*570*500mm
重量:56KG
电源:220V 50Hz
海门其林贝尔台式恒温振荡器THZ-D(已停产)
振荡频率:30-280次/分
振幅:旋转30mm
最大容量:1000ml*6 250ml*12
定时范围:0-999分 恒温范围:5-60℃
恒温精度:0.2℃ 温度均匀度:±0.5℃
输入功率:350W 外型尺寸:660*800*480mm
重量:45KG 电源:220V 50Hz
海门其林贝尔恒温振荡器THZ-C(已停产)
最大容量:6*1000ml(可自由调换)
转速:30-250P、R、M无级可调、自动保持(数显)
培养室温度:室温-60℃±0.1℃任选取(数显)
噪声:最高速运转,机前1MA声级50db
灯光:内装有普通照明灯,可对箱内进行光照处理
整机功率:300W 加热器250W
整机尺寸:500*610*490mm
电源:单相220V 50Hz
常州国华THZ-82水浴恒温振荡器,双数显
THZ-82水浴恒温振荡器(水浴恒温摇床)
【产品介绍】
是一种温度可控的恒温水浴槽和振荡器相结合的生化仪器,是植物、生物、微生物、遗传、病毒、环保、医学等科研、教育和生产部门作精密培养制备不可缺少的实验室设备
【技术参数】
型 号 |
THZ-82 |
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控温范围 (℃) |
室温~100℃ |
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温控精度 |
±0.5℃(双数显) |
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装瓶量 |
三角烧瓶250ml×12, 500ml×8 1000ml×4 |
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定时范围 |
0-120mins (或常开) |
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转 速 振 幅 |
起动 0-300r/min |
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振 幅mm |
20 (回旋) |
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整机功率 |
1800W |
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工作尺寸mm |
490*390*170 |
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外形尺寸mm |
700*550*490 |
常州国华THZ-82水浴恒温振荡器,单数显
THZ-82水浴恒温振荡器(水浴恒温摇床)
【产品介绍】
是一种温度可控的恒温水浴槽和振荡器相结合的生化仪器,是植物、生物、微生物、遗传、病毒、环保、医学等科研、教育和生产部门作精密培养制备不可缺少的实验室设备
【技术参数】
型 号 |
THZ-82 |
||
控温范围 (℃) |
室温~100℃ |
||
温控精度 |
±0.5℃(单数显) |
||
装瓶量 |
三角烧瓶250ml×12, 500ml×8 1000ml×4 |
||
定时范围 |
0-120mins (或常开) |
||
转 速 振 幅 |
起动 0-300r/min |
||
振 幅mm |
20 (回旋) |
||
整机功率 |
1800W |
||
工作尺寸mm |
490*390*170 |
||
外形尺寸mm |
700*550*490 |
上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。
THZ-P1-2 纯度: 99.06%
THZ-P1-2 是一种首创的,选择性的 PI5P4K 抑制剂,对 PI5P4Kα 的 IC50 为 190 nM。THZ-P1-2 共价靶向 PI5P4Kα/β/γ 中无序环上的半胱氨酸。THZ-P1-2 引起自噬破坏,上调 TFEB 信号传导。THZ-P1-2 在白血病细胞系中显示抗癌活性。
THZ-P1-2 Chemical Structure
CAS No. : 2058075-45-7
规格 | 价格 | 是否有货 | 数量 |
---|---|---|---|
5 mg | ¥4500 | In-stock | |
10 mg | ¥7800 | In-stock | |
50 mg | ¥22000 | In-stock | |
100 mg | ¥33000 | In-stock | |
200 mg | 询价 | ||
500 mg | 询价 |
* Please select Quantity before adding items.
生物活性 |
THZ-P1-2 is a first-in-class and selective PI5P4K inhibitor, with an IC50 of 190 nM for PI5P4Kα. THZ-P1-2 covalently targets cysteines on a disordered loop in PI5P4Kα/β/γ. THZ-P1-2 causes autophagy disruption and upregulates TFEB signaling. THZ-P1-2 displays anticancer activity in leukemia cell lines[1]. |
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体外研究 (In Vitro) |
THZ-P1-2 (0.2-11.4 μM) exhibits approximately 75% inhibition of PI-4,5-P2 formation by PI5P4Kα and PI5P4Kγ and 50% inhibition by PI5P4Kβ at a concentration of 0.7 μM[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation Assay[1]
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分子量 |
531.61 |
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Formula |
C31H29N7O2 |
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CAS 号 |
2058075-45-7 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
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溶解性数据 |
In Vitro:
DMSO : ≥ 250 mg/mL (470.27 mM) * “≥” means soluble, but saturation unknown. 配制储备液
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
|
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参考文献 |
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所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务
上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。
THZ1 纯度: 98.67%
THZ1 是有效,选择性的共价 CDK7 抑制剂,IC50 为 3.2 nM。THZ1 还抑制相关的激酶 CDK12 和 CDK13,并下调 MYC 表达。
THZ1 Chemical Structure
CAS No. : 1604810-83-4
规格 | 价格 | 是否有货 | 数量 |
---|---|---|---|
Free Sample (0.1-0.5 mg) | Apply now | ||
10 mM * 1 mL in DMSO | ¥934 | In-stock | |
5 mg | ¥750 | In-stock | |
10 mg | ¥1400 | In-stock | |
50 mg | ¥6000 | In-stock | |
100 mg | ¥11000 | In-stock | |
200 mg | 询价 | ||
500 mg | 询价 |
* Please select Quantity before adding items.
生物活性 |
THZ1 is a selective and potent covalent CDK7 inhibitor with an IC50 of 3.2 nM. THZ1 also inhibits the closely related kinases CDK12 and CDK13 and downregulates MYC expression[1][2]. |
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IC50 & Target |
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体外研究 (In Vitro) |
THZ1 inhibits Jurkat cell and Loucy cell with IC50 of 50 nM, and 0.55 nM, respectively. THZ1 (9, 27, 83, 250, 750, and 2500 nM) inhibits CDK12 but at higher concentrations compared to CDK7. THZ1 (1 μM) irreversibly inhibits RNAPII CTD and CAK phosphorylation. THZ1 (2.5 µM) irreversibly inhibits RNAPII CTD phosphorylation by covalently targeting a unique cysteine located outside the kinase domain of CDK7 in Hela S3 cells. THZ1 (250 nM) causes decreased cellular proliferation and an increase in apoptotic index with concomitant reduction in anti-apoptotic proteins, most notably MCL-1 and XIAP in T-ALL cell lines[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
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体内研究 (In Vivo) |
THZ1 (10 mg/kg) demonstrates potent killing of primary chronic lymphocytic leukemia (CLL) cells and anti-proliferative activity against primary TALL cells and in vivo against a human T-ALL xenograft[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
566.05 |
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Formula |
C31H28ClN7O2 |
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CAS 号 |
1604810-83-4 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
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溶解性数据 |
In Vitro:
DMSO : 100 mg/mL (176.66 mM; Need ultrasonic) Ethanol : < 1 mg/mL (insoluble) 配制储备液
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
|
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参考文献 |
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Cell Assay [1] |
Jurkat, Loucy, KOPTK1, and DND-41 cell lines are seeded in 384-well microplates at 15% confluency in medium. Cells are treated with THZ1 (2, 10, 50, 250, 1250, and 6250 nM) or DMSO for 72 hrs and cell viability is determined using resazurin[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
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Animal Administration [1] |
Mice[1] 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
参考文献 |
|
所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务
上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。
THZ531 纯度: 98.11%
THZ531 是选择性的 CDK12 和 CDK13 的共价抑制剂,IC50 值分别为 158 nM 和 69 nM。
THZ531 Chemical Structure
CAS No. : 1702809-17-3
规格 | 价格 | 是否有货 | 数量 |
---|---|---|---|
1 mg | ¥500 | In-stock | |
5 mg | ¥1000 | In-stock | |
10 mg | ¥1600 | In-stock | |
25 mg | ¥3500 | In-stock | |
50 mg | 询价 | ||
100 mg | 询价 |
* Please select Quantity before adding items.
生物活性 |
THZ531 is a selective and covalent inhibitor of both CDK12 and CDK13 with IC50s of 158 nM and 69 nM, respectively[1]. |
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IC50 & Target |
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体外研究 (In Vitro) |
The results from Kinase assays demonstrate that THZ531 potently inhibits CDK12 and CDK13 with IC50s of 158 nM and 69 nM, respectively; whereas inhibition of CDK7 and CDK9 is more than 50-fold weaker with IC50s of 8.5 and 10.5 µM, respectively. THZ531 treatment leads to a dramatic and irreversible decrease in Jurkat cell proliferation with an IC50 of 50 nM. FACS cell cycle analysis following treatment with escalating doses of THZ531 displays a dose and time-dependent increase in the number of cells exhibiting sub-G1 content. At 50 nM THZ531, no increase in the percentage of apoptotic cells is observed over DMSO control for the time course of the experiment. Higher doses of THZ531 leads to pronounced Annexin V signal with 30 to 40% annexin V-positively stained cells by 72 hrs. A dramatic reduction in elongating Pol II following THZ531 treatment is also observed[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
558.07 |
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Formula |
C30H32ClN7O2 |
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CAS 号 |
1702809-17-3 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
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溶解性数据 |
In Vitro:
DMSO : 14.29 mg/mL (25.61 mM; Need ultrasonic) 配制储备液
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
|
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参考文献 |
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Kinase Assay [1] |
Cells are treated with THZ531 or DMSO for 6 hrs. Following treatment cells are washed 2-fold with cold PBS and then lysed in the following lysis buffer: 50 mM Hepes pH 7.4, 150 mM NaCl, 1% Nonidet P40 substitute, 5 mM EDTA, 1 mM DTT, and protease/phosphatase cocktails. Following clearance, lysates are treated with bio-THZ1 or bio-TH531 for pulldown overnight at 4°C. Lysates are further incubated at room temperature for 3 hrs to increase the efficiency of covalent bond formation. Lysates are then incubated with streptavidin agarose for pulldown for an additional 2 to 3 hrs at 4°C[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
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Cell Assay [1] |
Jurkat cells are plated in 96-well plates at 20,000 cells/well in fresh media and treated with THZ531 or DMSO at the indicated concentrations for 72 hours. HAP1 cells are seeded in 96-well plates at 12,000 cells/well in fresh media and 24 hours later are treated with THZ531 at the indicated concentrations for 72 hours. Anti-proliferative effect of THZ531 is assessed. To assess the effect of inhibitor washout on anti-proliferation of Jurkat cells, cells are treated with THZ531 or DMSO for 6 hrs. Inhibitor-containing medium is then removed and incubated with or without THZ531 for 66 hrs. Anti-proliferative effect of THZ531 is assessed. All proliferation assays are performed in biological triplicate. IC50s are determined using non-linear regression curve fit[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
参考文献 |
|
所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务
上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。
THZ2 纯度: 99.62%
THZ2 是有效,选择性的 CDK7 抑制剂,IC50 值为 13.9 nM。
THZ2 Chemical Structure
CAS No. : 1604810-84-5
规格 | 价格 | 是否有货 | 数量 |
---|---|---|---|
10 mM * 1 mL in DMSO | ¥4110 | In-stock | |
5 mg | ¥3300 | In-stock | |
10 mg | ¥4900 | In-stock | |
50 mg | ¥14000 | In-stock | |
100 mg | 询价 |
* Please select Quantity before adding items.
生物活性 |
THZ2 is a potent and selective CDK7 inhibitor with an IC50 of 13.9 nM. |
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IC50 & Target[1] |
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体外研究 (In Vitro) |
THZ2 selectively targets CDK7 and potently inhibits the growth of triple-negative but not ER/PR+ breast cancer cells. THZ2 at low nanomolar doses also efficiently suppresses the clonogenic growth of TNBC cells with IC50 of appr 10 nM. THZ2 induces apoptotic cell death in triple-negative but not ER/PR+ breast cancer cells or normal human cells[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
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体内研究 (In Vivo) |
THZ2 (10 mg/Kg) markedly reduces the growth rate of tumors in mice and demonstrates an anti-tumor activity. Compared to vehicle-treated tumors, tumor tissues isolated from mice treated with THZ2 has reduced proliferation and increased apoptosis, as indicated by immunostaining against Ki67 and cleaved Caspase 3 respectively. THZ2 in NOD-SCID mice leads to reduced body weight, suggesting that THZ2 mayt be less well-tolerated in this particular mouse strain[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
566.05 |
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Formula |
C31H28ClN7O2 |
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CAS 号 |
1604810-84-5 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
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溶解性数据 |
In Vitro:
DMSO : 21.67 mg/mL (38.28 mM; Need ultrasonic) 配制储备液
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
|
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参考文献 |
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Cell Assay [1] |
For 96-well plate assay, cells are plated at the density of 2000 cells per well, and on the next day treated with THZ1 or THZ2 of various concentrations. After 48-hour incubation, cells are fixed and stained with crystal violet. The staining is then extracted by adding each well with 10% acetic acid, with absorbance measured at 590 nm with 750 nm as a reference. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
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Animal Administration [1] |
Mice: Nude mice (CrTac:NCr-Foxn1nu) are γ-irradiated with a single dose of 400 rads six hours before transplantation of cells. Breast cancer cells are harvested and resupended in 40% Matrigel-Basement Membrane Matrix, LDEV-free, and then injected (100 μL per site) into the fourth pair of mammary fat pads of mice. Tumors are measured in two dimensions by using manual calipers. Tumor volume is calculated using the formula: V=0.5× length × width × width. Animal with tumor established (mean tumor volume of appr 200 mm3) are randomLy divided into two groups, which are then treated with vehicle (10% DMSO in D5W, 5% dextrose in water) or THZ2 (3 mg/mL, prepared in vehicle solutions) at the dose of 10 mg/kg intraperitoneally twice daily. Tumor volume is measure every 2-3 days. Upon harvesting tumors, tumors are cut into half, with one half fixed in formalin overnight and then in 70% ethanol for histopathology analysis, and the other half snap frozen in liquid nitrogen for immunoblotting. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
参考文献 |
|
所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务
上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。
THZ1-R
THZ1-R是THZ1的非半胱氨酸反应性类似物,对 CDK7 抑制的活性降低。 THZ1-R与 CDK7 结合的 Kd 为142 nM。
THZ1-R Chemical Structure
CAS No. : 1621523-07-6
规格 | 价格 | 是否有货 | 数量 |
---|---|---|---|
1 mg | ¥1500 | In-stock | |
5 mg | ¥3500 | In-stock | |
10 mg | ¥5500 | In-stock | |
50 mg | ¥16500 | In-stock | |
100 mg | ¥23000 | In-stock | |
200 mg | 询价 | ||
500 mg | 询价 |
* Please select Quantity before adding items.
生物活性 |
THZ1-R is a non-cysteine reactive analog of THZ1 which displays diminished activity for CDK7 inhibition. THZ1-R binds to CDK7 with a Kd of 142 nM. |
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IC50 & Target |
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体外研究 (In Vitro) |
THZ1-R shows lower affinity at CDK7 than THZ1, with IC50 of 146 nM[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
568.07 |
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Formula |
C31H30ClN7O2 |
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CAS 号 |
1621523-07-6 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
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溶解性数据 |
In Vitro:
DMSO : ≥ 100 mg/mL (176.03 mM) * “≥” means soluble, but saturation unknown. 配制储备液
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
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参考文献 |
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Cell Assay [1] |
Cells are seeded in 384-well microplates at 15% confluency in medium with 5% FBS and penicillin/streptavidin. Cells are treated with THZ1 or DMSO for 72 hrs and cell viability is determined using resazurin. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
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参考文献 |
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所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务
上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。
THZ1 Hydrochloride 纯度: 98.49%
THZ1 Hydrochloride 是有效,选择性的共价 CDK7 抑制剂,IC50 为 3.2 nM。THZ1 Hydrochloride 还抑制相关的激酶 CDK12 和 CDK13,并下调 MYC 表达。
THZ1 Hydrochloride Chemical Structure
规格 | 价格 | 是否有货 | 数量 |
---|---|---|---|
Free Sample (0.1-0.5 mg) | Apply now | ||
10 mM * 1 mL in DMSO | ¥994 | In-stock | |
5 mg | ¥750 | In-stock | |
10 mg | ¥1400 | In-stock | |
50 mg | ¥6000 | In-stock | |
100 mg | ¥11000 | In-stock | |
200 mg | 询价 | ||
500 mg | 询价 |
* Please select Quantity before adding items.
生物活性 |
THZ1 Hydrochloride is a selective and potent covalent CDK7 inhibitor with an IC50 of 3.2 nM. THZ1 Hydrochloride also inhibits the closely related kinases CDK12 and CDK13 and downregulates MYC expression[1][2]. |
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IC50 & Target |
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体外研究 (In Vitro) |
THZ1 inhibits Jurkat cell and Loucy cell with IC50 of 50 nM, and 0.55 nM, respectively. THZ1 demonstrates time-dependent inhibition of CDK7 in vitro and covalent binding of intracellular CDK7. THZ1 (9, 27, 83, 250, 750, and 2500 nM) inhibits CDK12 but at higher concentrations compared to CDK7. THZ1 (1 μM) irreversibly inhibits RNAPII CTD and CAK phosphorylation. THZ1 (2.5 µM) irreversibly inhibits RNAPII CTD phosphorylation by covalently targeting a unique cysteine located outside the kinase domain of CDK7 in Hela S3 cells. THZ1 (250 nM) causes decreased cellular proliferation and an increase in apoptotic index with concomitant reduction in anti-apoptotic proteins, most notably MCL-1 and XIAP in T-ALL cell lines[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
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体内研究 (In Vivo) |
THZ1 (10 mg/kg) demonstrates potent killing of primary chronic lymphocytic leukemia (CLL) cells and anti-proliferative activity against primary TALL cells and in vivo against a human T-ALL xenograft[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
602.51 |
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Formula |
C31H29Cl2N7O2 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
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溶解性数据 |
In Vitro:
DMSO : 22.5 mg/mL (37.34 mM; Need ultrasonic and warming) H2O : < 0.1 mg/mL (ultrasonic;warming;heat to 60°C) (insoluble) 配制储备液
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
|
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参考文献 |
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Kinase Assay [1] |
For kinase assays following immunoprecipitation of FLAG-CDK7 protein from HCT116 or FLAG-CDK12 from 293A cellular lysates, cells are first treated with THZ1, THZ1-R, or DMSO for 4 hrs at 37°C. Cells are then harvested by lysis in 50 mM Tris HCl pH 8.0, 150 mM NaCl, 1% NP-40, 5 mM EDTA, and protease/phosphatase cocktails. Exogenous CDK7 or CDK12 proteins are immunoprecipitated from cellular lysates using FLAG antibody- conjugated agarose beads. Precipitated proteins are washed with lysis buffer 6 times, followed by 2 washes with kinase buffer (40 mM Hepes pH 7.5, 150 mM NaCl, 10 mM MgCl2, 5% glycerol) and subjected to in vitro kinase assays at 30°C for 45 minutes using 1 μg of the large subunit of RNAPII (RPB1) as substrate and 25 μM ATP and 10 μCi of 32P ATP. Note: no additional inhibitors are added to the kinase reaction mixture, therefore any inhibition results from the activity of the compounds that are added directly to cells (ie-intracellular inhibition). This suggests that the inhibitors are either covalently tethered to the kinase or have strong non-covalent character. Kinase assays using recombinant CDK7/TFIIH/MAT1 are conducted in the manner as described above using 25 ng of CAK complex per reaction. For kinase assays designed to test time-dependent inactivation of CDK7 kinase activity, CAK complex is pre-incubated with indicated concentrations of THZ1, THZ1-R, or DMSO in kinase buffer without ATP for 4 hrs at 30°C prior to being subjected to kinase assay conditions[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
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Cell Assay [1] |
Jurkat, Loucy, KOPTK1, and DND-41 cell lines are seeded in 384-well microplates at 15% confluency in medium with 5% FBS and penicillin/streptavidin. Cells are treated with THZ1 (2, 10, 50, 250, 1250, and 6250 nM) or DMSO for 72 hrs and cell viability is determined using resazurin[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
Animal Administration [1] |
Mice[1] 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only. |
参考文献 |
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所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务
【简单介绍】
【详细说明】
【简单介绍】
【详细说明】
一恒YIHENG 恒温振荡器——液晶屏 THZ-98C(双层)
◆人性化设计 ●集培养箱、振荡器于一体,占地小。 ●大屏幕液晶显示屏,能连续、精确、实时显示温度以及转速和工作时间,并且菜单式操作界面,简单易懂。(THZ-103B,THZ-100不具有此功能) ●顺应世界环保潮流,全新无氟设计,使你始终走在健康生活的前沿。 ●工程塑料外壳,大观察视窗,箱体内胆及振动台面均采用不锈钢材料,便于清洗。 ◆品质保证 ●采用微电脑控制温度和振荡频率,带有定时功能。 ●压缩机和循环风扇等关键零部件均采用进口产品,环保无氟制冷剂。 |
◆连续运行保证 ●低散热无碳刷直流电机,启动转矩大,调速宽、免保养、突破现有国产摇瓶机无法长时间连续运行的缺陷。 ◆安全功能 ●箱门丹启时,微风循环、加热和摇床自动停止,无温度过冲之弊。 ●独特控制转速电路,能确保摇床平稳启动,并能防止液体溅出而造成仪器损坏。 ●独立限温报警系统,超过限制温度后自动切断加热,保证安全运行不发生意外。(选配) ●循环风扇速度大小自动控制,可避免试验过程中,由于循环风扇过快而造成样品挥发。 ●配有高效过滤器,可拆下,易清洗,有效过滤外界空气中的细菌及灰尘颗粒,降低交叉污染。(选配) ●可配有RS-485接口和通过连接电脑,监测温度、转速、时间和报警。(选配) ◆多段可编程控制(选配) ●多段温度、速度、时间同步编程,普通和编程运行模式可选,预置值和运行值同 时显示,可以简化复杂的培养要求,真正实现自动控制和运行。 |
|
产品型号 |
THZ-98A |
THZ-98AB |
THZ-98C |
HZQ-X300 |
HZQ-X300C |
|
器皿规格 |
(单层) |
(双层) |
(双层) |
(双层) |
(双层) |
|
三角烧瓶(只) |
50(ml) |
29 |
29 |
29 |
37 |
37 |
100(ml) |
18 |
18 |
18 |
22 |
22 |
|
250(ml) |
11 |
11 |
11 |
14 |
14 |
|
500(ml) |
7 |
7 |
7 |
10 |
10 |
|
1000(ml) |
4 |
4 |
4 |
6 |
6 |
品牌简介:
上海一恒科学仪器有限公司创建于1997年,至今,已有十四万台“一恒仪器”的产品在全球30多个国家运行。公司一贯秉承“以科技保证品质,服务完善产品”的宗旨,领导中国潮流、主导行业动向。
一恒拥有自主品牌:,2000年后陆续通过了ISO 9001质量体系认证、ISO 13485医疗器械行业质量体系认证和CE认证,并获得“国家高新技术企业”认定,取得近30项外型、实用新型专利证书。
2010年销量突破22000台,其中出口5500台。一恒公司现已是中国最大的实验仪器制造商之一。生产基地位于昆山市千灯镇,总占地面积20000平方米,具备年产量30000台的制造能力,并获得“江苏省民营科技技术企业”荣誉称号。
2009年又在上海市级工业开发区——青浦园区内新建一处5000多平方米厂房,作为公司新产品研发和生产中心。
在全国建立了近120个分销点,在上海、北京、广东、成都、沈阳、哈尔滨、大连、郑州等地建立了强大售后维修服务体系。
一恒拥有强大的营销服务网络,并将目标瞄准国际市场,产品已销往德国、意大利、俄罗斯、土耳其、韩国、以色列、巴西、阿根廷、委内瑞拉、伊朗、伊拉克、阿联酋、印度、巴基斯坦、泰国、印度尼西亚、马来西亚、埃及、尼日利亚等三十多个国家和地区,赢得了国内外客户的广泛认同。
一恒本着“以客户服务”为导向,为客户提供实验室仪器及电子行业生产设备的整体解决方案和专业化服务。
商品属性 | |
---|---|
商品名称 | 一恒YIHENG 恒温振荡器—液晶屏 THZ-98C(双层)-THZ-98C(双层)-一恒YIHENG |
型号 | THZ-98C(双层) |
类别 | 实验室常用设备|||温度控制|||摇床/振荡器|||一恒YIHENG恒温振荡器—液晶屏THZ-98C(双层) |
品牌 | 一恒YIHENG |
品牌简介 | 一恒YIHENG |
关键字 | 国产实验室恒温振荡器 恒温摇床,振荡器,温度,恒温,转速,风扇,振幅 |