标签归档：aldehyde

HRP, aldehyde activated Cat. No. HRP-AL-1 1 mg修饰性聚乙二醇

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HRP, aldehyde activated

Cat. No. HRP-AL-1
Specification
Unit Size 1 mg
Price $385.00

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Description:

Nanocs provides a variety of chemically functionalized and bioconjugated Horseradish Peroxidase (HRP) for bioassay development. These functional HRP products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

  • 2~7 functional groups on each HRP molecule.
  • HRP conjugates provided in freeze dried powder;

Storage Conditions:

  • Store at 4 0C. Protect from light.

DSPE PEG Aldehyde,DSPE-PEG-CHO Cat. No. PG2-ALDS-5k 5000 Da 50 mg修饰性聚乙二醇

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DSPE PEG Aldehyde,DSPE-PEG-CHO

Cat. No. PG2-ALDS-5k
Specification 5000 Da
Unit Size 50 mg
Price $385.00

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Synonym: 1,2-distearoyl-sn-glycero-3-phosphoethanolamine PEG aldehyde, DSPE-PEG-CHO, Aldehyde PEG DSPE

Description:

DSPE PEG aldehyde (DSPE-PEG-CHO) is one of Nanocs’ reactive phospholipid PEG derivatives that can react with N-terminus amine groups. DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) is an 18 carbon phospholipid that is highly hydrophobic. PEG backbone, on the other hand, offers good hydrophilicity. Aldehyde PEG DSPE is a reactive lipid PEG with good reactivity towards amine, oxyamine or hydrazide groups. Aldehyde reacts with N-terminus amine groups to form an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Aldehyde PEG DSPE allows conjugation of DSPE PEG to amine or hydrazide group at acidic conditions with good selectivity. Higher pH will result in multiple pegylation with amine groups.

Products Information:

  • Form: Solid;
  • Color: White;
  • Reactive group: Aldehyde (-CHO)
  • Reactive to: N-terminus amine
  • Purity: >90%
  • Solubility: Soluble in warm water;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
  • PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

DSPE PEG Aldehyde Cat. No. PG2-ALDS-3k 3400 Da 50 mg修饰性聚乙二醇

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DSPE PEG Aldehyde

Cat. No. PG2-ALDS-3k
Specification 3400 Da
Unit Size 50 mg
Price $385.00

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Synonym: 1,2-distearoyl-sn-glycero-3-phosphoethanolamine PEG aldehyde, DSPE-PEG-CHO, Aldehyde PEG DSPE

Description:

DSPE PEG aldehyde (DSPE-PEG-CHO) is one of Nanocs’ reactive phospholipid PEG derivatives that can react with N-terminus amine groups. DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) is an 18 carbon phospholipid that is highly hydrophobic. PEG backbone, on the other hand, offers good hydrophilicity. Aldehyde PEG DSPE is a reactive lipid PEG with good reactivity towards amine, oxyamine or hydrazide groups. Aldehyde reacts with N-terminus amine groups to form an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Aldehyde PEG DSPE allows conjugation of DSPE PEG to amine or hydrazide group at acidic conditions with good selectivity. Higher pH will result in multiple pegylation with amine groups.

Products Information:

  • Form: Solid;
  • Color: White;
  • Reactive group: Aldehyde (-CHO)
  • Reactive to: N-terminus amine
  • Purity: >90%
  • Solubility: Soluble in warm water;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
  • PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

DSPE PEG Aldehyde Cat. No. PG2-ALDS-2k 2000 Da 50 mg修饰性聚乙二醇

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DSPE PEG Aldehyde

Cat. No. PG2-ALDS-2k
Specification 2000 Da
Unit Size 50 mg
Price $385.00

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Synonym: 1,2-distearoyl-sn-glycero-3-phosphoethanolamine PEG aldehyde, DSPE-PEG-CHO, Aldehyde PEG DSPE

Description:

DSPE PEG aldehyde (DSPE-PEG-CHO) is one of Nanocs’ reactive phospholipid PEG derivatives that can react with N-terminus amine groups. DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine) is an 18 carbon phospholipid that is highly hydrophobic. PEG backbone, on the other hand, offers good hydrophilicity. Aldehyde PEG DSPE is a reactive lipid PEG with good reactivity towards amine, oxyamine or hydrazide groups. Aldehyde reacts with N-terminus amine groups to form an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Aldehyde PEG DSPE allows conjugation of DSPE PEG to amine or hydrazide group at acidic conditions with good selectivity. Higher pH will result in multiple pegylation with amine groups.

Products Information:

  • Form: Solid;
  • Color: White;
  • Reactive group: Aldehyde (-CHO)
  • Reactive to: N-terminus amine
  • Purity: >90%
  • Solubility: Soluble in warm water;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
  • PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Cholesterol PEG aldehyde, CLS-PEG-CHO Cat. No. PG2-ALCS-5k 5000 Da 50 mg修饰性聚乙二醇

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Cholesterol PEG aldehyde, CLS-PEG-CHO

Cat. No. PG2-ALCS-5k
Specification 5000 Da
Unit Size 50 mg
Price $385.00

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Description:

Nanocs Cholesterol PEG aldehyde is a lipid PEG derivative with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

  • Off-white/white solid;
  • Soluble in warm water, DMSO, DMF;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
  • PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Cholesterol PEG aldehyde, CLS-PEG-CHO Cat. No. PG2-ALCS-3k 3400 Da 50 mg修饰性聚乙二醇

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Cholesterol PEG aldehyde, CLS-PEG-CHO

Cat. No. PG2-ALCS-3k
Specification 3400 Da
Unit Size 50 mg
Price $385.00

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Description:

Nanocs Cholesterol PEG aldehyde is a lipid PEG derivative with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

  • Off-white/white solid;
  • Soluble in warm water, DMSO, DMF;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
  • PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Cholesterol PEG aldehyde, CLS-PEG-CHO Cat. No. PG2-ALCS-2k 2000 Da 50 mg修饰性聚乙二醇

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Cholesterol PEG aldehyde, CLS-PEG-CHO

Cat. No. PG2-ALCS-2k
Specification 2000 Da
Unit Size 50 mg
Price $385.00

Qty Add to Cart

Description:

Nanocs Cholesterol PEG aldehyde is a lipid PEG derivative with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

  • Off-white/white solid;
  • Soluble in warm water, DMSO, DMF;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
  • PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Fluorescein PEG aldehyde Cat. No. PG2-ALFC-5k 5000 Da 10 mg修饰性聚乙二醇

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Fluorescein PEG aldehyde

Cat. No. PG2-ALFC-5k
Specification 5000 Da
Unit Size 10 mg
Price $385.00

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Fluorescein PEG Aldehyde, FITC-PEG-CHO

Fluorescein PEG Aldehde (FITC-PEG-CHO) is one of Nanocs’ reactive fluorescent PEG dyes that can react with N-terminal amines. Fluorescein is a bright green fluorescent dye with excitation/emission wavelength at 495 nm/515 nm. Aldehyde group is reactive toward amines, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of fuorescein dyes to desired position on targeted molecules. Conjugated FITC dye can be easily detected by common fluorescent instruments under FITC channel. PEG linker between FITC and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used fluorescent dyes, Nanocs pegylated fluorescent dyes are more stable and easier to use.

Physical Properties:

  • Appearance: Orange/yellow solid;
  • Solubility: Soluble in water, DMSO, DMF;
  • Reactive group: Aldehyde (-CHO);
  • Reactive toward: N-terminal amines, primary amine, aminooxy, hydrazide, etc.;
  • Ex/Em wavelength: 495 nm/515 nm;

Storage Conditions:

  • Store at -20 0C. Protect from light.

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Fluorescein PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
  • FITC PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted moleculess in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add FITC PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Fluorescein PEG aldehyde Cat. No. PG2-ALFC-3k 3400 Da 10 mg修饰性聚乙二醇

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Fluorescein PEG aldehyde

Cat. No. PG2-ALFC-3k
Specification 3400 Da
Unit Size 10 mg
Price $385.00

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Fluorescein PEG Aldehyde, FITC-PEG-CHO

Fluorescein PEG Aldehde (FITC-PEG-CHO) is one of Nanocs’ reactive fluorescent PEG dyes that can react with N-terminal amines. Fluorescein is a bright green fluorescent dye with excitation/emission wavelength at 495 nm/515 nm. Aldehyde group is reactive toward amines, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of fuorescein dyes to desired position on targeted molecules. Conjugated FITC dye can be easily detected by common fluorescent instruments under FITC channel. PEG linker between FITC and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used fluorescent dyes, Nanocs pegylated fluorescent dyes are more stable and easier to use.

Physical Properties:

  • Appearance: Orange/yellow solid;
  • Solubility: Soluble in water, DMSO, DMF;
  • Reactive group: Aldehyde (-CHO);
  • Reactive toward: N-terminal amines, primary amine, aminooxy, hydrazide, etc.;
  • Ex/Em wavelength: 495 nm/515 nm;

Storage Conditions:

  • Store at -20 0C. Protect from light.

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Fluorescein PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
  • FITC PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted moleculess in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add FITC PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Fluorescein PEG aldehyde Cat. No. PG2-ALFC-2k 2000 Da 10 mg修饰性聚乙二醇

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Fluorescein PEG aldehyde

Cat. No. PG2-ALFC-2k
Specification 2000 Da
Unit Size 10 mg
Price $425.00

Qty Add to Cart

Fluorescein PEG Aldehyde, FITC-PEG-CHO

Fluorescein PEG Aldehde (FITC-PEG-CHO) is one of Nanocs’ reactive fluorescent PEG dyes that can react with N-terminal amines. Fluorescein is a bright green fluorescent dye with excitation/emission wavelength at 495 nm/515 nm. Aldehyde group is reactive toward amines, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of fuorescein dyes to desired position on targeted molecules. Conjugated FITC dye can be easily detected by common fluorescent instruments under FITC channel. PEG linker between FITC and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used fluorescent dyes, Nanocs pegylated fluorescent dyes are more stable and easier to use.

Physical Properties:

  • Appearance: Orange/yellow solid;
  • Solubility: Soluble in water, DMSO, DMF;
  • Reactive group: Aldehyde (-CHO);
  • Reactive toward: N-terminal amines, primary amine, aminooxy, hydrazide, etc.;
  • Ex/Em wavelength: 495 nm/515 nm;

Storage Conditions:

  • Store at -20 0C. Protect from light.

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Fluorescein PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine-free aqueous buffer, pH 5.0~6.5.
  • FITC PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted moleculess in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add FITC PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

m-PEG3-aldehyde

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上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

m-PEG3-aldehyde 

m-PEG3-aldehyde 是一种 PROTAC linker (PROTAC linker)，属于 PEG 类，可用于合成 PROTACs。

m-PEG3-aldehyde

m-PEG3-aldehyde Chemical Structure

CAS No. : 356066-46-1

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

m-PEG3-aldehyde is a PEG-based PROTAC linker can be used in the synthesis of PROTACs.

IC50 & Target

PEGs

 

体外研究
(In Vitro)

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].
The binding affinities of anti-mPEGs depend more on the backbone lengths of the polymers and the hydrophobicities of their end-groups than on their resemblance to the methoxy terminus of the immunogenic polymer[1].
Aldehyde is reactive to hydrazide and aminooxy groups which are commonly used in biomolecular probes for labeling and crosslinking carbonyls (oxidized carbohydrates). The hydrophilic PEG spacer increases solubility in aqueous media.

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

176.21

Formula

C8H16O4
CAS 号

356066-46-1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Saifer MG, et al.Selectivity of binding of PEGs and PEG-like oligomers to anti-PEG antibodies induced by methoxyPEG-proteins.Mol Immunol. 2014 Feb;57(2):236-46.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

m-PEG4-CH2-aldehyde

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

m-PEG4-CH2-aldehyde 

m-PEG4-CH2-aldehyde 是一种基于 PEG 的 PROTAC linker，用于 PROTAC 合成。

m-PEG4-CH2-aldehyde

m-PEG4-CH2-aldehyde Chemical Structure

CAS No. : 1059189-65-9

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

m-PEG4-CH2-aldehyde is a PEG-based based PROTAC linker can be used in the synthesis of PROTACs.

IC50 & Target[1]

PEGs

 

体外研究
(In Vitro)

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

250.29

Formula

C11H22O6
CAS 号

1059189-65-9
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Jennifer Riggs-Sauthier, et al. Oligomer-protease inhibitor conjugates. WO2008112289A2.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Biotin PEG aldehyde Cat. No. PG2-ALBN-5k 5000 Da 10 mg修饰性聚乙二醇

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Biotin PEG aldehyde

Cat. No. PG2-ALBN-5k
Specification 5000 Da
Unit Size 10 mg
Price $385.00

Qty Add to Cart

Biotin PEG Aldehyde

Biotin PEG Aldehde (Biotin-PEG-CHO) is one of Nanocs’ chemical reactive biotin PEG derivatives that can label N-terminal amines. Aldehyde group is reactive toward amine, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with protonated amines at acidic environment (pH ~4), a condition sometimes required for certain site-specific biotinylation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of biotin tag to desired position on targeted molecules. Resulted biotin can recognize and bind to avidin, streptavidin or neutravidin with high affinity. PEG linker between Biotin and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used biotinylation reagents, Nanocs pegylated biotins are water soluble, more stable and easier to use.

Physical Properties:

  • Appearance: White/off-whilte solid;
  • Solubility: Soluble in water, DMSO, DMF;
  • Reacive group: Aldehyde (-CHO);
  • Reactive to: N-terminal amine, primary amine, etc.;
  • Functional moiety: D-biotin;
  • Reactive toward: avidin, streptavidin or neutravidin;

Storage Conditions:

  • Store at -20 0C. Desiccate.

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Biotin PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.

Materials Required:

  • Reaction buffer: Amine-free aqueous buffer, pH 5.0~6.5.
  • Biotin PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve ready labeling molecules in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add Biotin PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Biotin PEG aldehyde, Aldehyde PEG Biotin Cat. No. PG2-ALBN-3k 3400 Da 10 mg修饰性聚乙二醇

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Biotin PEG aldehyde, Aldehyde PEG Biotin

Cat. No. PG2-ALBN-3k
Specification 3400 Da
Unit Size 10 mg
Price $385.00

Qty Add to Cart

Biotin PEG Aldehyde

Biotin PEG Aldehde (Biotin-PEG-CHO) is one of Nanocs’ chemical reactive biotin PEG derivatives that can label N-terminal amines. Aldehyde group is reactive toward amine, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with protonated amines at acidic environment (pH ~4), a condition sometimes required for certain site-specific biotinylation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of biotin tag to desired position on targeted molecules. Resulted biotin can recognize and bind to avidin, streptavidin or neutravidin with high affinity. PEG linker between Biotin and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used biotinylation reagents, Nanocs pegylated biotins are water soluble, more stable and easier to use.

Physical Properties:

  • Appearance: White/off-whilte solid;
  • Solubility: Soluble in water, DMSO, DMF;
  • Reacive group: Aldehyde (-CHO);
  • Reactive to: N-terminal amine, primary amine, etc.;
  • Functional moiety: D-biotin;
  • Reactive toward: avidin, streptavidin or neutravidin;

Storage Conditions:

  • Store at -20 0C. Desiccate.

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Biotin PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.

Materials Required:

  • Reaction buffer: Amine-free aqueous buffer, pH 5.0~6.5.
  • Biotin PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve ready labeling molecules in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add Biotin PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Biotin PEG aldehyde, Aldehyde PEG Biotin Cat. No. PG2-ALBN-2k 2000 Da 10 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Biotin PEG aldehyde, Aldehyde PEG Biotin

Cat. No. PG2-ALBN-2k
Specification 2000 Da
Unit Size 10 mg
Price $385.00

Qty Add to Cart

Biotin PEG Aldehyde

Biotin PEG Aldehde (Biotin-PEG-CHO) is one of Nanocs’ chemical reactive biotin PEG derivatives that can label N-terminal amines. Aldehyde group is reactive toward amine, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with protonated amines at acidic environment (pH ~4), a condition sometimes required for certain site-specific biotinylation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of biotin tag to desired position on targeted molecules. Resulted biotin can recognize and bind to avidin, streptavidin or neutravidin with high affinity. PEG linker between Biotin and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used biotinylation reagents, Nanocs pegylated biotins are water soluble, more stable and easier to use.

Physical Properties:

  • Appearance: White/off-whilte solid;
  • Solubility: Soluble in water, DMSO, DMF;
  • Reacive group: Aldehyde (-CHO);
  • Reactive to: N-terminal amine, primary amine, etc.;
  • Functional moiety: D-biotin;
  • Reactive toward: avidin, streptavidin or neutravidin;

Storage Conditions:

  • Store at -20 0C. Desiccate.

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Biotin PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.

Materials Required:

  • Reaction buffer: Amine-free aqueous buffer, pH 5.0~6.5.
  • Biotin PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve ready labeling molecules in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add Biotin PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Maleimide PEG aldehyde Cat. No. PG2-ALML-5k 5000 Da 10 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Maleimide PEG aldehyde

Cat. No. PG2-ALML-5k
Specification 5000 Da
Unit Size 10 mg
Price $385.00

Qty Add to Cart

Description:

Maleimide PEG aldehyde is one of Nanocs premier thiol (-SH) and N-terminal amine group pegylation reagents. Maleimide group reacts with sulfhydryl (thiol) group at pH 6.5-7.5 rapidly and specifically while aldehyde group reacts with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Pegylated maleimide and aldehydes are water soluble and all reactions can be carried out in aqueous buffer. Aldehyde reacts with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

  • White/off-white solid;
  • Soluble in warm water, DMSO, DMF;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Maleimide PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine and thiol free aqueous buffer, pH 5.0~6.5.
  • Maleimide PEG aldehyde stock solution: 20 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add maleimide PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Maleimide PEG aldehyde Cat. No. PG2-ALML-3k 3400 Da 10 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Maleimide PEG aldehyde

Cat. No. PG2-ALML-3k
Specification 3400 Da
Unit Size 10 mg
Price $385.00

Qty Add to Cart

Description:

Maleimide PEG aldehyde is one of Nanocs premier thiol (-SH) and N-terminal amine group pegylation reagents. Maleimide group reacts with sulfhydryl (thiol) group at pH 6.5-7.5 rapidly and specifically while aldehyde group reacts with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Pegylated maleimide and aldehydes are water soluble and all reactions can be carried out in aqueous buffer. Aldehyde reacts with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

  • White/off-white solid;
  • Soluble in warm water, DMSO, DMF;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Maleimide PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine and thiol free aqueous buffer, pH 5.0~6.5.
  • Maleimide PEG aldehyde stock solution: 20 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add maleimide PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Maleimide PEG aldehyde Cat. No. PG2-ALML-2k 2000 Da 10 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Maleimide PEG aldehyde

Cat. No. PG2-ALML-2k
Specification 2000 Da
Unit Size 10 mg
Price $385.00

Qty Add to Cart

Description:

Maleimide PEG aldehyde is one of Nanocs premier thiol (-SH) and N-terminal amine group pegylation reagents. Maleimide group reacts with sulfhydryl (thiol) group at pH 6.5-7.5 rapidly and specifically while aldehyde group reacts with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Pegylated maleimide and aldehydes are water soluble and all reactions can be carried out in aqueous buffer. Aldehyde reacts with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

  • White/off-white solid;
  • Soluble in warm water, DMSO, DMF;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Maleimide PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine and thiol free aqueous buffer, pH 5.0~6.5.
  • Maleimide PEG aldehyde stock solution: 20 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add maleimide PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

m-PEG4-aldehyde

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

m-PEG4-aldehyde 

m-PEG4-aldehyde 是一种 PROTAC linker，属于 PEG 类。可用于合成 PROTAC 分子。

m-PEG4-aldehyde

m-PEG4-aldehyde Chemical Structure

CAS No. : 197513-96-5

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生物活性

m-PEG4-aldehyde is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1].

IC50 & Target

PEGs

 

体外研究
(In Vitro)

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

220.26

Formula

C10H20O5
CAS 号

197513-96-5
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562

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