Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.
Physical Properties:
Organge/yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Similar products: Alexa Fluor 488 DNase, SF488 DNase.
Product Description:
Fluorescein labeled DNase I is one of Nanocs’ fluorescent DNase conjugates. Deoxyribonuclease I, also called DNase I, is a endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide. DNase is konown to be involved in apoptosisis and plays a role in actin polymerization. Nanocs provides a number of fluorescent labeled DNase I products that span from UV region to near IR. These fluorescent DNase conjugates can be used for enzyme studies, bioassay development and many other biomedical research. Nanocs Fluorescein labeled DNase I, FITC DNase is a green fluorescent DNase I conjugate that has excitation/emission wavelength at 490/520 nm. This fluorescein DNase enzyme can be easily detected by fluorescent instruments in FITC channel. All our fluorescent DNase conjugates were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.
Product Specifications:
DNase source: Bovine pancreas.
2~5 fluorescein dyes on each DNase molecule.
Molecular weight: ~29.1 kD
DNase I conjugates provided either in water solution and dried powder;
Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.
Physical Properties:
Organge/yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.
Physical Properties:
Organge/yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.
Physical Properties:
Organge/yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.
Physical Properties:
Organge/yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Fluorescein (Uranine) is widely used as a fluorescent tracer in medicinal and biological applications and tumor infected tissues tracer. Fluorescein (Uranine) is a representative green fluorophore that has been widely used as a scaffold of practically useful green fluorescent probes[1][2].
体外研究 (In Vitro)
Fluorescein is a synthetic organic photoactive dye compound soluble in water, alcohol and polar solvents[1].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量
332.31
Formula
C20H12O5
CAS 号
2321-07-5
中文名称
荧光素；荧光黄；荧光红；荧光橙；酸性黄73
运输条件
Room temperature in continental US; may vary elsewhere.
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献
[1]. Nabel A Negm, et al. Fluorescein dye derivatives and their nanohybrids: Synthesis, characterization and antimicrobial activity. J Photochem Photobiol B. 2016 Sep;162:421-433.
[2]. Li Liu, et al. Fluorescein as an artificial enzyme to mimic peroxidase. Chem Commun (Camb). 2016 Nov24;52(96):13912-13915.
[3]. RobertSjöback, et al. Absorption and fluorescence properties of fluorescein. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Fluorescein PEG Aldehde (FITC-PEG-CHO) is one of Nanocs’ reactive fluorescent PEG dyes that can react with N-terminal amines. Fluorescein is a bright green fluorescent dye with excitation/emission wavelength at 495 nm/515 nm. Aldehyde group is reactive toward amines, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of fuorescein dyes to desired position on targeted molecules. Conjugated FITC dye can be easily detected by common fluorescent instruments under FITC channel. PEG linker between FITC and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used fluorescent dyes, Nanocs pegylated fluorescent dyes are more stable and easier to use.
Generally, a 5- to 10-fold molar excess of Fluorescein PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.
FITC PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
Washing solution: Distilled water or any aqueous buffer.
Reaction Steps:
Dissolve targeted moleculess in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add FITC PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.
Fluorescein PEG Aldehde (FITC-PEG-CHO) is one of Nanocs’ reactive fluorescent PEG dyes that can react with N-terminal amines. Fluorescein is a bright green fluorescent dye with excitation/emission wavelength at 495 nm/515 nm. Aldehyde group is reactive toward amines, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of fuorescein dyes to desired position on targeted molecules. Conjugated FITC dye can be easily detected by common fluorescent instruments under FITC channel. PEG linker between FITC and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used fluorescent dyes, Nanocs pegylated fluorescent dyes are more stable and easier to use.
Generally, a 5- to 10-fold molar excess of Fluorescein PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.
FITC PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
Washing solution: Distilled water or any aqueous buffer.
Reaction Steps:
Dissolve targeted moleculess in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add FITC PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.
Fluorescein PEG Aldehde (FITC-PEG-CHO) is one of Nanocs’ reactive fluorescent PEG dyes that can react with N-terminal amines. Fluorescein is a bright green fluorescent dye with excitation/emission wavelength at 495 nm/515 nm. Aldehyde group is reactive toward amines, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of fuorescein dyes to desired position on targeted molecules. Conjugated FITC dye can be easily detected by common fluorescent instruments under FITC channel. PEG linker between FITC and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used fluorescent dyes, Nanocs pegylated fluorescent dyes are more stable and easier to use.
Generally, a 5- to 10-fold molar excess of Fluorescein PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.
FITC PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
Washing solution: Distilled water or any aqueous buffer.
Reaction Steps:
Dissolve targeted moleculess in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add FITC PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.
Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.
Physical Properties:
Organge/yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, desiccated Protect from light. Avoid frequent thaw and freeze.
Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.
Physical Properties:
Organge/yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, desiccated Protect from light. Avoid frequent thaw and freeze.
Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.
Physical Properties:
Organge/yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, desiccated Protect from light. Avoid frequent thaw and freeze.
Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.
Physical Properties:
Organge/yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, desiccated Protect from light. Avoid frequent thaw and freeze.
N-(Azido-PEG3)-N-Fluorescein-PEG3-acid Chemical Structure
CAS No. : 2100306-50-9
规格
是否有货
1 mg
询价
* Please select Quantity before adding items.
生物活性
N-(Azido-PEG3)-N-Fluorescein-PEG3-acid is a PEG-based PROTAC linker which contains azide, fluorescein and carboxylic acid moieties.
IC50 & Target
PEGs
体外研究 (In Vitro)
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
811.85
Formula
C38H45N5O13S
CAS 号
2100306-50-9
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.
Physical Properties:
Organge/yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, desiccated Protect from light. Avoid frequent thaw and freeze.
Fluorescein-thiourea-PEG4-azide Chemical Structure
CAS No. : 1454662-54-4
规格
是否有货
100 mg
询价
250 mg
询价
500 mg
询价
* Please select Quantity before adding items.
生物活性
Fluorescein-thiourea-PEG4-azide is a PEG-based PROTAC linker can be used in the synthesis of PROTACs.
IC50 & Target
PEGs
体外研究 (In Vitro)
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
651.69
Formula
C31H33N5O9S
CAS 号
1454662-54-4
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
Fluorescein-PEG5-acid is a PEG-based PROTAC linker can be used in the synthesis of PROTACs.
IC50 & Target
PEGs
体外研究 (In Vitro)
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
698.74
Formula
C34H38N2O12S
CAS 号
2183440-40-4
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
Fluorescein-thiourea-PEG6-acid is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1].
IC50 & Target
PEGs
体外研究 (In Vitro)
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
742.79
Formula
C36H42N2O13S
CAS 号
2055014-69-0
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562
