m-PEG3-aldehyde is a PEG-based PROTAC linker can be used in the synthesis of PROTACs.
IC50 & Target
PEGs
体外研究 (In Vitro)
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1]. The binding affinities of anti-mPEGs depend more on the backbone lengths of the polymers and the hydrophobicities of their end-groups than on their resemblance to the methoxy terminus of the immunogenic polymer[1]. Aldehyde is reactive to hydrazide and aminooxy groups which are commonly used in biomolecular probes for labeling and crosslinking carbonyls (oxidized carbohydrates). The hydrophilic PEG spacer increases solubility in aqueous media.
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
176.21
Formula
C8H16O4
CAS 号
356066-46-1
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Saifer MG, et al.Selectivity of binding of PEGs and PEG-like oligomers to anti-PEG antibodies induced by methoxyPEG-proteins.Mol Immunol. 2014 Feb;57(2):236-46.
m-PEG4-CH2-aldehyde is a PEG-based based PROTAC linker can be used in the synthesis of PROTACs.
IC50 & Target[1]
PEGs
体外研究 (In Vitro)
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
250.29
Formula
C11H22O6
CAS 号
1059189-65-9
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Jennifer Riggs-Sauthier, et al. Oligomer-protease inhibitor conjugates. WO2008112289A2.
Biotin PEG Aldehde (Biotin-PEG-CHO) is one of Nanocs’ chemical reactive biotin PEG derivatives that can label N-terminal amines. Aldehyde group is reactive toward amine, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with protonated amines at acidic environment (pH ~4), a condition sometimes required for certain site-specific biotinylation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of biotin tag to desired position on targeted molecules. Resulted biotin can recognize and bind to avidin, streptavidin or neutravidin with high affinity. PEG linker between Biotin and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used biotinylation reagents, Nanocs pegylated biotins are water soluble, more stable and easier to use.
Physical Properties:
Appearance: White/off-whilte solid;
Solubility: Soluble in water, DMSO, DMF;
Reacive group: Aldehyde (-CHO);
Reactive to: N-terminal amine, primary amine, etc.;
Functional moiety: D-biotin;
Reactive toward: avidin, streptavidin or neutravidin;
Storage Conditions:
Store at -20 0C. Desiccate.
Reaction Procedures:
Generally, a 5- to 10-fold molar excess of Biotin PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.
Biotin PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
Washing solution: Distilled water or any aqueous buffer.
Reaction Steps:
Dissolve ready labeling molecules in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add Biotin PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.
Biotin PEG Aldehde (Biotin-PEG-CHO) is one of Nanocs’ chemical reactive biotin PEG derivatives that can label N-terminal amines. Aldehyde group is reactive toward amine, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with protonated amines at acidic environment (pH ~4), a condition sometimes required for certain site-specific biotinylation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of biotin tag to desired position on targeted molecules. Resulted biotin can recognize and bind to avidin, streptavidin or neutravidin with high affinity. PEG linker between Biotin and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used biotinylation reagents, Nanocs pegylated biotins are water soluble, more stable and easier to use.
Physical Properties:
Appearance: White/off-whilte solid;
Solubility: Soluble in water, DMSO, DMF;
Reacive group: Aldehyde (-CHO);
Reactive to: N-terminal amine, primary amine, etc.;
Functional moiety: D-biotin;
Reactive toward: avidin, streptavidin or neutravidin;
Storage Conditions:
Store at -20 0C. Desiccate.
Reaction Procedures:
Generally, a 5- to 10-fold molar excess of Biotin PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.
Biotin PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
Washing solution: Distilled water or any aqueous buffer.
Reaction Steps:
Dissolve ready labeling molecules in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add Biotin PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.
Biotin PEG Aldehde (Biotin-PEG-CHO) is one of Nanocs’ chemical reactive biotin PEG derivatives that can label N-terminal amines. Aldehyde group is reactive toward amine, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with protonated amines at acidic environment (pH ~4), a condition sometimes required for certain site-specific biotinylation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of biotin tag to desired position on targeted molecules. Resulted biotin can recognize and bind to avidin, streptavidin or neutravidin with high affinity. PEG linker between Biotin and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used biotinylation reagents, Nanocs pegylated biotins are water soluble, more stable and easier to use.
Physical Properties:
Appearance: White/off-whilte solid;
Solubility: Soluble in water, DMSO, DMF;
Reacive group: Aldehyde (-CHO);
Reactive to: N-terminal amine, primary amine, etc.;
Functional moiety: D-biotin;
Reactive toward: avidin, streptavidin or neutravidin;
Storage Conditions:
Store at -20 0C. Desiccate.
Reaction Procedures:
Generally, a 5- to 10-fold molar excess of Biotin PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.
Biotin PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
Washing solution: Distilled water or any aqueous buffer.
Reaction Steps:
Dissolve ready labeling molecules in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add Biotin PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.
Maleimide PEG aldehyde is one of Nanocs premier thiol (-SH) and N-terminal amine group pegylation reagents. Maleimide group reacts with sulfhydryl (thiol) group at pH 6.5-7.5 rapidly and specifically while aldehyde group reacts with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Pegylated maleimide and aldehydes are water soluble and all reactions can be carried out in aqueous buffer. Aldehyde reacts with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.
Physical Properties:
White/off-white solid;
Soluble in warm water, DMSO, DMF;
Storage Conditions:
Store at -20 0C
Reaction Procedures:
Generally, a 5- to 10-fold molar excess of Maleimide PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.
Materials Required:
Pegylation buffer: Amine and thiol free aqueous buffer, pH 5.0~6.5.
Maleimide PEG aldehyde stock solution: 20 mg in 1 mL conjugation buffer.
Washing solution: Distilled water or any aqueous buffer.
Reaction Steps:
Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add maleimide PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.
Maleimide PEG aldehyde is one of Nanocs premier thiol (-SH) and N-terminal amine group pegylation reagents. Maleimide group reacts with sulfhydryl (thiol) group at pH 6.5-7.5 rapidly and specifically while aldehyde group reacts with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Pegylated maleimide and aldehydes are water soluble and all reactions can be carried out in aqueous buffer. Aldehyde reacts with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.
Physical Properties:
White/off-white solid;
Soluble in warm water, DMSO, DMF;
Storage Conditions:
Store at -20 0C
Reaction Procedures:
Generally, a 5- to 10-fold molar excess of Maleimide PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.
Materials Required:
Pegylation buffer: Amine and thiol free aqueous buffer, pH 5.0~6.5.
Maleimide PEG aldehyde stock solution: 20 mg in 1 mL conjugation buffer.
Washing solution: Distilled water or any aqueous buffer.
Reaction Steps:
Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add maleimide PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.
Maleimide PEG aldehyde is one of Nanocs premier thiol (-SH) and N-terminal amine group pegylation reagents. Maleimide group reacts with sulfhydryl (thiol) group at pH 6.5-7.5 rapidly and specifically while aldehyde group reacts with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Pegylated maleimide and aldehydes are water soluble and all reactions can be carried out in aqueous buffer. Aldehyde reacts with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.
Physical Properties:
White/off-white solid;
Soluble in warm water, DMSO, DMF;
Storage Conditions:
Store at -20 0C
Reaction Procedures:
Generally, a 5- to 10-fold molar excess of Maleimide PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.
Materials Required:
Pegylation buffer: Amine and thiol free aqueous buffer, pH 5.0~6.5.
Maleimide PEG aldehyde stock solution: 20 mg in 1 mL conjugation buffer.
Washing solution: Distilled water or any aqueous buffer.
Reaction Steps:
Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add maleimide PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.
m-PEG4-aldehyde is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1].
IC50 & Target
PEGs
体外研究 (In Vitro)
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
220.26
Formula
C10H20O5
CAS 号
197513-96-5
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562
Z-Ile-Leu-aldehyde (Z-IL-CHO) is a potent and competitive peptide aldehyde inhibitor of γ-secretase and notch[1][2].
体外研究 (In Vitro)
Z-Ile-Leu-aldehyde (ILCHO) significantly downregulates Th17-associated cytokine levels in murine Th17 in vitro polarization assays[1]. Z-Ile-Leu-aldehyde (GSI XII) induces apoptosis of murine MOPC315.BM myeloma cells with high Notch activity[2].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
RT-PCRsup>[1]
Cell Line:
CD4+ T cells from C57BL/6 mice.
Concentration:
25 μM.
Incubation Time:
24, 48, 72 hours.
Result:
DownregulateD RORt and IL-17 mRNA expression.
Cell Viability Assaysup>[2]
Cell Line:
MOPC315.BM cells.
Concentration:
0, 12, 15 μM.
Incubation Time:
24-48 h hours.
Result:
Reduced viability and induced apoptosis in MOPC315.BM cells
体内研究 (In Vivo)
Z-Ile-Leu-aldehyde (GSI XII, 10 mg/kg, Intraperitoneally either for 14 days) controls myeloma bone disease mainly by targeting Notch in MM cells and possibly in osteoclasts in their microenvironment[2].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
MOPC315.BM mouse model[2].
Dosage:
10 mg/kg.
Administration:
Intraperitoneally either for 14 days.
Result:
Reduces myeloma-specific paraprotein levels in the MOPC315.BM model. Diminished osteolytic lesions in the MOPC315.BM mice.
分子量
362.46
Formula
C20H30N2O4
CAS 号
161710-10-7
运输条件
Room temperature in continental US; may vary elsewhere.
Aldehyde-benzyl-PEG5-alkyne is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1].
IC50 & Target
PEGs
体外研究 (In Vitro)
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1]
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
350.41
Formula
C19H26O6
CAS 号
1378928-83-6
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562.
