Thioredoxin reductase peptide

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Thioredoxin reductase peptide 

Thioredoxin reductase peptide 是硫氧还蛋白还原酶的 53–67 残基对应的多肽,用于硫氧还蛋白还原酶研究。硫氧还蛋白还原酶作为含二硫化物蛋白质的还原剂,在细胞抗氧化防御中起着重要作用。

Thioredoxin reductase peptide

Thioredoxin reductase peptide Chemical Structure

CAS No. : 950890-23-0

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Thioredoxin reductase peptide 的其他形式现货产品:

Thioredoxin reductase peptide TFA

生物活性

Thioredoxin reductase peptide corresponds to residues 53–67 in thioredoxin reductase (TrxR), used in thioredoxin reductase research. Thioredoxin reductase acts as a reductant of disulfide-containing proteins and plays crucial role in cellular antioxidant defense[1][2].

分子量

1503.79

Formula

C66H106N18O18S2

CAS 号

950890-23-0

Sequence

Trp-Gly-Leu-Gly-Gly-Thr-Cys-Val-Asn-Val-Gly-Cys-Ile-Pro-Lys

Sequence Shortening

WGLGGTCVNVGCIPK

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Jan YH, et al. Cross-linking of thioredoxin reductase by the sulfur mustard analogue mechlorethamine(methylbis(2-chloroethyl)amine) in human lung epithelial cells and rat lung: selective inhibition of disulfide reduction but not redox cycling. Chem Res Toxicol. 2014 Jan 21;27(1):61-75.

    [2]. Valette O, et al. Biochemical Function, Molecular Structure and Evolution of an Atypical Thioredoxin Reductase from Desulfovibrio vulgaris. Front Microbiol. 2017 Sep 29;8:1855.

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KEA1-97

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白,专注于信号通路和疾病研究领域。

KEA1-97  纯度: 99.66%

KEA1-97 是一种选择性Thioredoxin-caspase 3相互作用干扰剂 (IC50=10 μM)。KEA1-97 在不影响 thioredoxin 活性的情况下, 破坏 thioredoxin 与 caspase 3 的相互作用, 激活 caspases, 诱导细胞 apoptosis

KEA1-97

KEA1-97 Chemical Structure

CAS No. : 2138882-71-8

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg)   Apply now  
10 mM * 1 mL in DMSO ¥880 In-stock
5 mg ¥800 In-stock
10 mg ¥1300 In-stock
25 mg ¥2500 In-stock
50 mg ¥4500 In-stock
100 mg ¥7500 In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

KEA1-97 相关产品

相关化合物库:

  • Bioactive Compound Library Plus
  • Apoptosis Compound Library
  • Anti-Cancer Compound Library
  • Pyroptosis Compound Library
  • Neurodegenerative Disease-related Compound Library
  • Targeted Diversity Library

生物活性

KEA1-97 is a selective Thioredoxin-caspase 3 interaction disruptor (IC50=10 μM). KEA1-97 disrupts the interaction of thioredoxin with caspase 3, activates caspases, and induces apoptosis without affecting thioredoxin activity[1].

IC50 & Target[1]

Caspase 3

10 μM (IC50)

Thioredoxin

10 μM (IC50)

体外研究
(In Vitro)

KEA1-97 (100 μM; 231MFP cells) impaires thioredoxin pulldown of caspase 3[1].
KEA1-97 (10 μM; 48 hours; 231MFP cells) impaires 231MFP serum-free cell proliferation[1].
KEA1-97 (10 μM; 0~12 hours; 231MFP cells) activates caspase 3/7 and induces apoptotic cell death[1].
KEA1-97 (231MFP cells) resistants to survival and proliferation impairments[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: 231MFP cells
Concentration: 100 μM
Incubation Time:
Result: Impaired thioredoxin pulldown of caspase 3.

Cell Proliferation Assay[1]

Cell Line: 231MFP cells
Concentration: 10 μM
Incubation Time: 48 h
Result: Impaired 231MFP serum-free cell proliferation.

Apoptosis Analysis[1]

Cell Line: 231MFP cells
Concentration: 10 μM
Incubation Time: 0~12 h
Result: Activated caspase 3/7 and induced apoptotic cell death.

体内研究
(In Vivo)

KEA1-97 (5 mg/kg; i.p.; 50 days) attenuates tumor xenograft growth[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Immune-deficient SCID mice
Dosage: 5 mg/kg
Administration: I.p.; 50 days
Result: Attenuated tumor xenograft growth.

分子量

335.16

Formula

C15H9Cl2FN4

CAS 号

2138882-71-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : 100 mg/mL (298.36 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.9836 mL 14.9182 mL 29.8365 mL
5 mM 0.5967 mL 2.9836 mL 5.9673 mL
10 mM 0.2984 mL 1.4918 mL 2.9836 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (7.46 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (7.46 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献
  • [1]. Anderson KE, et al. Chemoproteomics-Enabled Covalent Ligand Screening Reveals a Thioredoxin-Caspase 3 Interaction Disruptor That Impairs Breast Cancer Pathogenicity. ACS Chem Biol. 2017;12(10):2522-2528.

所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务