Phosphoramide mustard 是环磷酰胺 (HY-17420) 的活性代谢物，具有抗肿瘤活性。Phosphoramide mustard 能诱导 DNA 损伤。
Phosphoramide mustard Chemical Structure
CAS No. : 10159-53-2
规格
是否有货
100 mg
询价
250 mg
询价
500 mg
询价
* Please select Quantity before adding items.
Phosphoramide mustard 的其他形式现货产品:
Phosphoramide mustard (cyclohexanamine)
生物活性
Phosphoramide mustard is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard induces DNA damage[1][2].
IC50 & Target
DNA Alkylator[1]
体外研究 (In Vitro)
Phosphoramide mustard causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication[1]. Phosphoramide mustard (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs)[1]. Phosphoramide mustard (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage[1]. Phosphoramide mustard (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[1]
Cell Line:
SIGCs
Concentration:
0.5 μM, 1 μM, 3 μM, 6 μM
Incubation Time:
48 hours
Result:
Reduced cell viability at concentrations of 3 μM and higher.
RT-PCR[1]
Cell Line:
SIGCs
Concentration:
3 μM, 6 μM
Incubation Time:
24 hours, 48 hours
Result:
Increased DDR gene mRNA expression levels.
Western Blot Analysis[1]
Cell Line:
SIGCs
Concentration:
3 μM, 6 μM
Incubation Time:
24 hours, 48 hours
Result:
Generally increased DDR proteins.
体内研究 (In Vivo)
Phosphoramide mustard (2.1-20.7 mg/kg; i.p.; daily; for 5 days) inhibits subcutaneous tumor growth in rats[2]. Phosphoramide mustard exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg)[2].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor[2]
Dosage:
2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg
Administration:
Intraperitoneal injection, once daily, for 5 consecutive days
Result:
Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.
Animal Model:
Rats[2]
Dosage:
59.4 mg/kg (Pharmacokinetic Analysis)
Administration:
Intravenous injection
Result:
T1/2 (15.1 min).
分子量
221.02
Formula
C4H11Cl2N2O2P
CAS 号
10159-53-2
中文名称
磷酰胺氮芥
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Shanthi Ganesan, et al. Phosphoramide mustard exposure induces DNA adduct formation and the DNA damage repair response in rat ovarian granulosa cells. Toxicol Appl Pharmacol. 2015 Feb 1; 282(3): 252–258.
[2]. S Genka, et al. Brain and plasma pharmacokinetics and anticancer activities of cyclophosphamide and phosphoramide mustard in the rat. Cancer Chemother Pharmacol. 1990;27(1):1-7.
Phosphoramide mustard 是环磷酰胺 (HY-17420) 的活性代谢物，具有抗肿瘤活性。Phosphoramide mustard 能诱导 DNA 损伤。
Phosphoramide mustard Chemical Structure
CAS No. : 10159-53-2
规格
是否有货
100 mg
询价
250 mg
询价
500 mg
询价
* Please select Quantity before adding items.
Phosphoramide mustard 的其他形式现货产品:
Phosphoramide mustard (cyclohexanamine)
生物活性
Phosphoramide mustard is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard induces DNA damage[1][2].
IC50 & Target
DNA Alkylator[1]
体外研究 (In Vitro)
Phosphoramide mustard causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication[1]. Phosphoramide mustard (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs)[1]. Phosphoramide mustard (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage[1]. Phosphoramide mustard (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins[1].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[1]
Cell Line:
SIGCs
Concentration:
0.5 μM, 1 μM, 3 μM, 6 μM
Incubation Time:
48 hours
Result:
Reduced cell viability at concentrations of 3 μM and higher.
RT-PCR[1]
Cell Line:
SIGCs
Concentration:
3 μM, 6 μM
Incubation Time:
24 hours, 48 hours
Result:
Increased DDR gene mRNA expression levels.
Western Blot Analysis[1]
Cell Line:
SIGCs
Concentration:
3 μM, 6 μM
Incubation Time:
24 hours, 48 hours
Result:
Generally increased DDR proteins.
体内研究 (In Vivo)
Phosphoramide mustard (2.1-20.7 mg/kg; i.p.; daily; for 5 days) inhibits subcutaneous tumor growth in rats[2]. Phosphoramide mustard exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg)[2].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor[2]
Dosage:
2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg
Administration:
Intraperitoneal injection, once daily, for 5 consecutive days
Result:
Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.
Animal Model:
Rats[2]
Dosage:
59.4 mg/kg (Pharmacokinetic Analysis)
Administration:
Intravenous injection
Result:
T1/2 (15.1 min).
分子量
221.02
Formula
C4H11Cl2N2O2P
CAS 号
10159-53-2
中文名称
磷酰胺氮芥
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Shanthi Ganesan, et al. Phosphoramide mustard exposure induces DNA adduct formation and the DNA damage repair response in rat ovarian granulosa cells. Toxicol Appl Pharmacol. 2015 Feb 1; 282(3): 252–258.
[2]. S Genka, et al. Brain and plasma pharmacokinetics and anticancer activities of cyclophosphamide and phosphoramide mustard in the rat. Cancer Chemother Pharmacol. 1990;27(1):1-7.
Phosphoramide mustard 是环磷酰胺 (HY-17420) 的活性代谢物，具有抗肿瘤活性。Phosphoramide mustard 能诱导 DNA 损伤。
Phosphoramide mustard Chemical Structure
CAS No. : 10159-53-2
规格
是否有货
100 mg
询价
250 mg
询价
500 mg
询价
* Please select Quantity before adding items.
Phosphoramide mustard 的其他形式现货产品:
Phosphoramide mustard (cyclohexanamine)
生物活性
Phosphoramide mustard is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard induces DNA damage[1][2].
IC50 & Target
DNA Alkylator[1]
体外研究 (In Vitro)
Phosphoramide mustard causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication[1]. Phosphoramide mustard (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs)[1]. Phosphoramide mustard (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage[1]. Phosphoramide mustard (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[1]
Cell Line:
SIGCs
Concentration:
0.5 μM, 1 μM, 3 μM, 6 μM
Incubation Time:
48 hours
Result:
Reduced cell viability at concentrations of 3 μM and higher.
RT-PCR[1]
Cell Line:
SIGCs
Concentration:
3 μM, 6 μM
Incubation Time:
24 hours, 48 hours
Result:
Increased DDR gene mRNA expression levels.
Western Blot Analysis[1]
Cell Line:
SIGCs
Concentration:
3 μM, 6 μM
Incubation Time:
24 hours, 48 hours
Result:
Generally increased DDR proteins.
体内研究 (In Vivo)
Phosphoramide mustard (2.1-20.7 mg/kg; i.p.; daily; for 5 days) inhibits subcutaneous tumor growth in rats[2]. Phosphoramide mustard exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg)[2].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor[2]
Dosage:
2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg
Administration:
Intraperitoneal injection, once daily, for 5 consecutive days
Result:
Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.
Animal Model:
Rats[2]
Dosage:
59.4 mg/kg (Pharmacokinetic Analysis)
Administration:
Intravenous injection
Result:
T1/2 (15.1 min).
分子量
221.02
Formula
C4H11Cl2N2O2P
CAS 号
10159-53-2
中文名称
磷酰胺氮芥
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Shanthi Ganesan, et al. Phosphoramide mustard exposure induces DNA adduct formation and the DNA damage repair response in rat ovarian granulosa cells. Toxicol Appl Pharmacol. 2015 Feb 1; 282(3): 252–258.
[2]. S Genka, et al. Brain and plasma pharmacokinetics and anticancer activities of cyclophosphamide and phosphoramide mustard in the rat. Cancer Chemother Pharmacol. 1990;27(1):1-7.
Phosphoramide mustard (cyclohexanamine) Chemical Structure
CAS No. : 1566-15-0
规格
价格
是否有货
数量
5 mg
￥3400
In-stock
10 mg
￥6000
In-stock
25 mg
￥12500
In-stock
50 mg
询价
100 mg
询价
* Please select Quantity before adding items.
Phosphoramide mustard (cyclohexanamine) 相关产品
•相关化合物库:
Bioactive Compound Library Plus
Cell Cycle/DNA Damage Compound Library
Metabolism/Protease Compound Library
Anti-Cancer Compound Library
Anti-Aging Compound Library
Anti-Lung Cancer Compound Library
Drug Metabolite Library
生物活性
Phosphoramide mustard cyclohexanamine is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard cyclohexanamine induces DNA damage[1][2].
体外研究 (In Vitro)
Phosphoramide mustard cyclohexanamine causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication[1]. Phosphoramide mustard cyclohexanamine (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs)[1]. Phosphoramide mustard cyclohexanamine (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage[1]. Phosphoramide mustard cyclohexanamine (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins[1].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[1]
Cell Line:
SIGCs
Concentration:
0.5 μM, 1 μM, 3 μM, 6 μM
Incubation Time:
48 hours
Result:
Reduced cell viability at concentrations of 3 μM and higher.
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor[2]
Dosage:
2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg
Administration:
Intraperitoneal injection, once daily, for 5 consecutive days
Result:
Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.
Animal Model:
Rats[2]
Dosage:
86.0 mg/kg (Pharmacokinetic Analysis)
Administration:
Intravenous injection
Result:
T1/2 (15.1 min).
分子量
320.20
Formula
C10H24Cl2N3O2P
CAS 号
1566-15-0
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20°C, protect from light, stored under nitrogen
*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:
H2O : 100 mg/mL (312.30 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
3.1230 mL
15.6152 mL
31.2305 mL
5 mM
0.6246 mL
3.1230 mL
6.2461 mL
10 mM
0.3123 mL
1.5615 mL
3.1230 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献
[1]. Shanthi Ganesan, et al. Phosphoramide mustard exposure induces DNA adduct formation and the DNA damage repair response in rat ovarian granulosa cells. Toxicol Appl Pharmacol. 2015 Feb 1; 282(3): 252–258.
[2]. S Genka, et al. Brain and plasma pharmacokinetics and anticancer activities of cyclophosphamide and phosphoramide mustard in the rat. Cancer Chemother Pharmacol. 1990;27(1):1-7.
