标签归档：cannabigerol

O,O-Dimethyl-cannabigerol

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

O,O-Dimethyl-cannabigerol  纯度: 99.86%

O,O-Dimethyl-cannabigerol 是一种可从大麻中提取的天然物质。O,O-Dimethyl-cannabigerol 对金黄色葡萄球菌耐药菌株具有抗菌作用 (MIC 范围为 1-2 μg/mL)。O,O-Dimethyl-cannabigerol 属于非精神活性物质。

O,O-Dimethyl-cannabigerol

O,O-Dimethyl-cannabigerol Chemical Structure

CAS No. : 29106-16-9

规格 价格 是否有货 数量
5 mg ￥4800 In-stock
10 mg ￥7700 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

生物活性

O,O-Dimethyl-cannabigerol is a natural product from Cannabis sativa. O,O-Dimethyl-cannabigerol has an antibacterial effect on drug-resistant strains of Staphylococcus aureus (MIC ranging from 1 to 2 µg/mL)[1]. O,O-Dimethyl-cannabigerol is a nonpsychoactive constituent[2][3].

体外研究
(In Vitro)

O,O-Dimethyl-cannabigerol (compound 1) inhibits KB cells growth with IC50s of 44.55 μM (MTT assay) and 69.4 μM (SRB assay)[2].
O,O-Dimethyl-cannabigerol (0-100 μM; 48 hours) induces cell cytotoxic in NIH 3T3 fibroblasts, with CD50s of 60.46 μM (MTT assay) and 82.98 μM (SRB assay)[2].
O,O-Dimethyl-cannabigerol shows antitumor efficacy against melanoma cells of a mouse’s skin with an IC50 of 31.3 μM[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay[2]

Cell Line: NIH 3T3 fibroblasts
Concentration: 0, 1, 25, 50, 100 μM
Incubation Time: 48 hours
Result: Induced cell cytotoxic with CD50s of 60.46 μM (MTT assay) and 82.98 μM (SRB assay)

分子量

344.53

Formula

C23H36O2
CAS 号

29106-16-9
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 55 mg/mL (159.64 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.9025 mL 14.5125 mL 29.0250 mL
5 mM 0.5805 mL 2.9025 mL 5.8050 mL
10 mM 0.2903 mL 1.4513 mL 2.9025 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.75 mg/mL (7.98 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.75 mg/mL (7.98 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例，取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.75 mg/mL (7.98 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.75 mg/mL (7.98 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例，取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2.75 mg/mL (7.98 mM); Clear solution

    此方案可获得 ≥ 2.75 mg/mL (7.98 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献

  • [1]. Appendino G, et al. Antibacterial cannabinoids from Cannabis sativa: a structure-activity study. J Nat Prod. 2008;71(8):1427-1430.

    [2]. Han DS, et al. Synthesis and cytotoxic effects of deoxy-tomentellin. Arch Pharm Res. 2000;23(2):121-127.

    [3]. Caprioglio D, et al. O-Methyl Phytocannabinoids: Semi-synthesis, Analysis in Cannabis Flowerheads, and Biological Activity. Planta Med. 2019;85(11-12):981-986.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Cannabigerol

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Cannabigerol 

Cannabigerol 是一种主要的植物大麻素，在 CB1 和 CB2 受体上表现为弱部分激动剂。Cannabigerol 也是一种相对有效和高效的 TRPA1 激动剂(EC50=0.7 μM)，TRPV1 和 TRPV2 的弱激动剂 (分别为 1.3 和 1.72 μM)，以及有效的 TRPM8 (0.16 μM) 和 5-HT1A 受体拮抗剂。Cannabigerol是一种有效的 α2-肾上腺素受体激动剂 (0.2 nM)。Cannabigerol促进 CRC 细胞凋亡 (apoptosis)，刺激 活性氧 ROS 产生，上调 CHOP mRNA，减少细胞生长。

Cannabigerol

Cannabigerol Chemical Structure

CAS No. : 25654-31-3

规格 价格 是否有货
1 mg ￥1500 询问价格 & 货期
5 mg ￥3750 询问价格 & 货期

* Please select Quantity before adding items.

生物活性

Cannabigerol, one of the major phytocannabinoids, behaves as a weak partial agonist at CB1 and CB2 receptors. Cannabigerol is also a relatively potent and highly effective TRPA1 agonist (EC50=0.7μM), a weak agonist at TRPV1 and TRPV2 (1.3 and 1.72 μM, respectively), and a potent TRPM8 (0.16 μM) and 5-HT1A receptor antagonist. Cannabigerol is a potent α2-adrenoceptor agonist (0.2 nM). Cannabigerol promotes apoptosis, stimulates reactive oxygen species (ROS) production, upregulates CHOP mRNA and reduces cell growth in CRC cells[1][2][3][4].

体外研究
(In Vitro)

Cannabigerol (CBG) antagonizes the 5-HT1A receptor agonist, R-(+)-8-hydroxy-2-(di-n-propylamino)tetralin (apparent KB=51.9 nM)[1].
In CHO cells stably expressing human receptors, Cannabigerol is a weak partial agonist of both CB1R- and CB2R-dependent inhibition of cAMP that displaying a low affinity for CB1R and comparatively higher affinity for CB2R (Ki= 1300 and 490 nM, respectively)[3]. Cannabigerol (1-30 μM; 3-48 hurs) reduces cell viability in human colorectal cancer (Caco-2) cells in a time- and serum protein concentration-dependent manner[3].
Cannabigerol (10 μM; 24 hours) induces apoptosis in colorectal cancer (Caco-2) cells[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Cannabigerol (1-10 mg/kg; intraperitoneally; every day for 5 days; athymic female mice bearing HCT 116 cells) reduces colon carcinogenesis in vivo[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

316.48

Formula

C21H32O2
CAS 号

25654-31-3
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Cascio MG, et al. Evidence that the plant cannabinoid cannabigerol is a highly potent alpha2-adrenoceptor agonist and moderately potent 5HT1A receptor antagonist. Br J Pharmacol. 2010;159(1):129-141.

    [2]. De Petrocellis L, et al. Effects of cannabinoids and cannabinoid-enriched Cannabis extracts on TRP channels and endocannabinoid metabolic enzymes. Br J Pharmacol. 2011;163(7):1479-1494.

    [3]. Borrelli F, et al. Colon carcinogenesis is inhibited by the TRPM8 antagonist cannabigerol, a Cannabis-derived non-psychotropic cannabinoid. Carcinogenesis. 2014;35(12):2787-2797.

    [4]. Zagzoog A, et al. In vitro and in vivo pharmacological activity of minor cannabinoids isolated from Cannabis sativa. Sci Rep. 2020;10(1):20405. Published 2020 Nov 23.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务