mPEG Desthiobiotin is one of Nanocs’ pegylated desthiobiotin PEG derivatives that is soluble in water. Desthiobiotin is a modified version of biotin. Compared to biotin, desthiobiotin binds to streptavidin, avidin and other biotin-binding proteins with lower affinity and it can be eluted from binding complex under much milder conditions (i.e., by competitive displacement with regular, free biotin). As such, desthiobiotin reagent is a useful alternative to Biotin for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. Pegylated desthiobiotin has good solubility and can be easily used in biotin/streptavidin assays and biomolecule purification process.
Physical Properties:
Off-white/white solid;
Soluble in water;
Binding group: Desthiobiotin;
Bind to: Streptavidin;
PEG type: PEG5000, linear;
Storage Conditions:
Store at -20 0C. Desiccate. Avoid frequent thaw and freeze.
Nanocs SnapLink DesthioBiotin NHS is an amine reactive derivative of desthiobiotin. Desthiobiotin has similar binding properties to avidin or strepatvidin as Biotin but it is elutable from streptavidin, making it ideal for protein purification and intracellular labeling. The desthiobiotin tag binds to streptavidin and other biotin-binding proteins with high specificity yet readily elutes with mild conditions (i.e., by competitive displacement with regular, free biotin). As such, this reagent is a useful alternative to Biotin-NHS for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. This simplest and smallest amine-reactive desthiobiotin reagent is uncharged and cell membrane permeable; therefore, NHS-Desthiobiotin can be used with intact cells for intracellular protein labeling. N-hydroxysuccinimide ester of desthiobiotin reacts efficiently with primary amine groups (-NH2) in alkaline buffer (pH 7-9).
Specifications:
Appearance (color): White.
Appearance (form): Powder;
Solubility: Soluble in DMSO, DMF, not soluble in water;
Desthiobiotin PEG NHS is one of Nanocs desthiobiotin PEG derivatives that can react with primary amine groups. Desthiobiotin has similar binding properties to avidin or strepatvidin as Biotin but it is elutable from streptavidin, making it ideal for protein purification and intracellular labeling. The desthiobiotin tag binds to streptavidin and other biotin-binding proteins with high specificity yet readily elutes with mild conditions (i.e., by competitive displacement with regular, free biotin). As such, this reagent is a useful alternative to Biotin-NHS for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. This simplest and smallest amine-reactive desthiobiotin reagent is uncharged and cell membrane permeable; therefore, NHS-Desthiobiotin can be used with intact cells for intracellular protein labeling. N-hydroxysuccinimide ester of desthiobiotin reacts efficiently with primary amine groups (-NH2) in alkaline buffer (pH 7-9). Pegylated desthiobiotin has a hydrophilic PEG chain between NHS and desthiobitin and thus offers higher water solubility and less steric hindrance.
Desthiobiotin PEG NHS is one of Nanocs desthiobiotin PEG derivatives that can react with primary amine groups. Desthiobiotin has similar binding properties to avidin or strepatvidin as Biotin but it is elutable from streptavidin, making it ideal for protein purification and intracellular labeling. The desthiobiotin tag binds to streptavidin and other biotin-binding proteins with high specificity yet readily elutes with mild conditions (i.e., by competitive displacement with regular, free biotin). As such, this reagent is a useful alternative to Biotin-NHS for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. This simplest and smallest amine-reactive desthiobiotin reagent is uncharged and cell membrane permeable; therefore, NHS-Desthiobiotin can be used with intact cells for intracellular protein labeling. N-hydroxysuccinimide ester of desthiobiotin reacts efficiently with primary amine groups (-NH2) in alkaline buffer (pH 7-9). Pegylated desthiobiotin has a hydrophilic PEG chain between NHS and desthiobitin and thus offers higher water solubility and less steric hindrance.
Desthiobiotin PEG amine is one of Nanocs desthiobiotin derivatives having a reactive primary amine group. Desthiobiotin has similar binding properties to avidin or strepatvidin as Biotin but it is elutable from streptavidin, making it ideal for protein purification and intracellular labeling. The desthiobiotin tag binds to streptavidin and other biotin-binding proteins with high specificity yet readily elutes with mild conditions (i.e., by competitive displacement with regular, free biotin). As such, this reagent is a useful alternative to Biotin for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. Pegylated desthiobiotin has a hydrophilic polyethyleneglycol spacer arm that imparts water solubility to entire molecules. Free primary amine group on the other end of PEG allows other amine reactive groups to be easily attached.
Desthiobiotin PEG amine is one of Nanocs desthiobiotin derivatives having a reactive primary amine group. Desthiobiotin has similar binding properties to avidin or strepatvidin as Biotin but it is elutable from streptavidin, making it ideal for protein purification and intracellular labeling. The desthiobiotin tag binds to streptavidin and other biotin-binding proteins with high specificity yet readily elutes with mild conditions (i.e., by competitive displacement with regular, free biotin). As such, this reagent is a useful alternative to Biotin for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. Pegylated desthiobiotin has a hydrophilic polyethyleneglycol spacer arm that imparts water solubility to entire molecules. Free primary amine group on the other end of PEG allows other amine reactive groups to be easily attached.
Desthiobiotin PEG amine is one of Nanocs desthiobiotin derivatives having a reactive primary amine group. Desthiobiotin has similar binding properties to avidin or strepatvidin as Biotin but it is elutable from streptavidin, making it ideal for protein purification and intracellular labeling. The desthiobiotin tag binds to streptavidin and other biotin-binding proteins with high specificity yet readily elutes with mild conditions (i.e., by competitive displacement with regular, free biotin). As such, this reagent is a useful alternative to Biotin for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. Pegylated desthiobiotin has a hydrophilic polyethyleneglycol spacer arm that imparts water solubility to entire molecules. Free primary amine group on the other end of PEG allows other amine reactive groups to be easily attached.
Desthiobiotin-PEG4-acid is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1].
IC50 & Target
PEGs
体外研究 (In Vitro)
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
461.55
Formula
C21H39N3O8
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562
Desthiobiotin PEG NHS is one of Nanocs’ reactive desthiobiotin PEG crosslinkers that can react with primary amine groups. Desthiobiotin is a modified version of biotin. Compared to biotin, desthiobiotin binds to streptavidin, avidin or neutraviain with lower affinity and thus it can be eluted under milder conditions (i.e., by competitive displacement with regular, free biotin). As such, desthiobiotin reagent is a useful alternative to Biotin for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. Succinimidyl ester (NHS) reacts specifically and efficiently with primary amine group (-NH2) at alkaline pH to form a stable amide bond. Reaction between NHS and amine allows quick and efficient conjugation of desthiobiotin tag to proteins, antibodies and other biomolecules. Resulted desthibiotin conjugates can be used for bioassay development, biomolecule separation and purification. PEG linker between desthiobiotin and maleimide offers better water solubility, reduced steric hindrance and enhanced stability.
Physical Properties:
Off-white/white solid;
Soluble in water, DMSO or DMF;
Reactive group: Succinimidyl ester (NHS);
Reactive toward: Primary amine (-NH2).
Storage Conditions:
Store at -20 0C. Desiccate. Avoid frequent thaw and freeze.
Desthiobiotin PEG NHS is one of Nanocs’ reactive desthiobiotin PEG crosslinkers that can react with primary amine groups. Desthiobiotin is a modified version of biotin. Compared to biotin, desthiobiotin binds to streptavidin, avidin or neutraviain with lower affinity and thus it can be eluted under milder conditions (i.e., by competitive displacement with regular, free biotin). As such, desthiobiotin reagent is a useful alternative to Biotin for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. Succinimidyl ester (NHS) reacts specifically and efficiently with primary amine group (-NH2) at alkaline pH to form a stable amide bond. Reaction between NHS and amine allows quick and efficient conjugation of desthiobiotin tag to proteins, antibodies and other biomolecules. Resulted desthibiotin conjugates can be used for bioassay development, biomolecule separation and purification. PEG linker between desthiobiotin and maleimide offers better water solubility, reduced steric hindrance and enhanced stability.
Physical Properties:
Off-white/white solid;
Soluble in water, DMSO or DMF;
Reactive group: Succinimidyl ester (NHS);
Reactive toward: Primary amine (-NH2).
Storage Conditions:
Store at -20 0C. Desiccate. Avoid frequent thaw and freeze.
Desthiobiotin PEG NHS is one of Nanocs’ reactive desthiobiotin PEG crosslinkers that can react with primary amine groups. Desthiobiotin is a modified version of biotin. Compared to biotin, desthiobiotin binds to streptavidin, avidin or neutraviain with lower affinity and thus it can be eluted under milder conditions (i.e., by competitive displacement with regular, free biotin). As such, desthiobiotin reagent is a useful alternative to Biotin for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. Succinimidyl ester (NHS) reacts specifically and efficiently with primary amine group (-NH2) at alkaline pH to form a stable amide bond. Reaction between NHS and amine allows quick and efficient conjugation of desthiobiotin tag to proteins, antibodies and other biomolecules. Resulted desthibiotin conjugates can be used for bioassay development, biomolecule separation and purification. PEG linker between desthiobiotin and maleimide offers better water solubility, reduced steric hindrance and enhanced stability.
Physical Properties:
Off-white/white solid;
Soluble in water, DMSO or DMF;
Reactive group: Succinimidyl ester (NHS);
Reactive toward: Primary amine (-NH2).
Storage Conditions:
Store at -20 0C. Desiccate. Avoid frequent thaw and freeze.
Desthiobiotin PEG Maleimide is one of Nanocs’ reactive desthiobiotin PEG crosslinkers that can react with thiol (-SH) groups. Maleimide reacts with thiol/sulfhydryl group at neutral pH quckily and efficiently. Reaction between maleimide and thiol allows conjugation of desthiobiotin tag to proteins, antibodies, peptides or nucleic acids with high efficiency. Desthiobiotin is a modified version of biotin. Compared to biotin, desthiobiotin binds to streptavidin, avidin and other biotin-binding proteins with lower affinity and it can be eluted from binding complex under much milder conditions (i.e., by competitive displacement with regular, free biotin). As such, desthiobiotin reagent is a useful alternative to Biotin for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. PEG linker between desthiobiotin and maleimide offers better water solubility, less steric hindrance and enhanced stability.
Physical Properties:
Off-white/white solid;
Soluble in water, DMSO or DMF;
Reactive group: Maleimide;
Reactive to: Thiol (-SH);
Storage Conditions:
Store at -20 0C. Desiccate. Avoid frequent thaw and freeze.
Desthiobiotin PEG Maleimide is one of Nanocs’ reactive desthiobiotin PEG crosslinkers that can react with thiol (-SH) groups. Maleimide reacts with thiol/sulfhydryl group at neutral pH quckily and efficiently. Reaction between maleimide and thiol allows conjugation of desthiobiotin tag to proteins, antibodies, peptides or nucleic acids with high efficiency. Desthiobiotin is a modified version of biotin. Compared to biotin, desthiobiotin binds to streptavidin, avidin and other biotin-binding proteins with lower affinity and it can be eluted from binding complex under much milder conditions (i.e., by competitive displacement with regular, free biotin). As such, desthiobiotin reagent is a useful alternative to Biotin for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. PEG linker between desthiobiotin and maleimide offers better water solubility, less steric hindrance and enhanced stability.
Physical Properties:
Off-white/white solid;
Soluble in water, DMSO or DMF;
Reactive group: Maleimide;
Reactive to: Thiol (-SH);
Storage Conditions:
Store at -20 0C. Desiccate. Avoid frequent thaw and freeze.
Desthiobiotin PEG Maleimide is one of Nanocs’ reactive desthiobiotin PEG crosslinkers that can react with thiol (-SH) groups. Maleimide reacts with thiol/sulfhydryl group at neutral pH quckily and efficiently. Reaction between maleimide and thiol allows conjugation of desthiobiotin tag to proteins, antibodies, peptides or nucleic acids with high efficiency. Desthiobiotin is a modified version of biotin. Compared to biotin, desthiobiotin binds to streptavidin, avidin and other biotin-binding proteins with lower affinity and it can be eluted from binding complex under much milder conditions (i.e., by competitive displacement with regular, free biotin). As such, desthiobiotin reagent is a useful alternative to Biotin for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. PEG linker between desthiobiotin and maleimide offers better water solubility, less steric hindrance and enhanced stability.
Physical Properties:
Off-white/white solid;
Soluble in water, DMSO or DMF;
Reactive group: Maleimide;
Reactive to: Thiol (-SH);
Storage Conditions:
Store at -20 0C. Desiccate. Avoid frequent thaw and freeze.
Desthiobiotin PEG amine (DSB-PEG-NH2) is one of Nanocs’ desthiobiotin PEG crosslinkers that have a reactive primary amine group on one PEG terminus and a desthiobiotin tag on the other PEG end. Desthiobiotin is a modified version of biotin. Compared to biotin, desthiobiotin binds to streptavidin, avidin and other biotin-binding proteins with lower affinity and it can be eluted from binding complex with much more gental conditions (i.e., by competitive displacement with regular, free biotin). As such, desthiobiotin reagent is a useful alternative to Biotin for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. Amine functionalized desthiobiotin can react with a number of amine reactive groups such as succinimidyl ester, carboxylic acid, aldehyde, etc. Reaction between amine and other groups allows quick and efficient conjugation of desthiobiotin tag to proteins, antibodies and other biomolecules. PEG linker between desthiobiotin and amine group offers better water solubility, reduced steric hindrance and enhanced reactivity.
Physical Properties:
Off-white/white solid;
Soluble in aqueous buffer, DMSO or DMF;
Reactive group: Primary amine (-NH2);
Reactive to: NHS, aldehyde, COOH, etc.;
Storage Conditions:
Store at <-20 0C. Desiccate. Avoid frequent thaw and freeze.
Desthiobiotin PEG Thiol is one of Nanocs’ thiolated desthiobiotin PEG derivatives that can bind to gold particle surface with high affinity. It can also react with a number of thiol reactive groups such as maleimide, iodoacetyl and pyridyldithio, etc. Reactions bewteen thiol and gold or other groups allows conjugation of desthiobiotin tag to targeted molecule or materials substrates quickly and efficiently. Resulted desthiobiotin can be used to bind to avdidin or streptavidin. PEG linker between thiol and desthiobiotin offers better water solubility, reduced steric hindrance and ehanced reactivity.
Physical Properties:
Off-white/white solid;
Soluble in aqeous solution, DMSO or DMF;
Reactive group: Thiol (-SH);
Reactive to: Maleimide, Iodoacetyl, Pyridyldithio, etc.;
Storage Conditions:
Store at -20 0C. Desiccate. Avoid frequent thaw and freeze.
Desthiobiotin PEG Azide is one of Nanocs’ reactive desthiobiotin derivatives. Azide is a commonly used click chemistry functional group that can react with alkyne catalyzed by copper ion. It can also react with strain-promoted cyclooctyne such as DBCO spontaneously without need any catalyst. Reaction between azide and alkyne allows attachment of destionbiotin tag to targeted molecules or material substrates with high efficiency. Resulted desthiobiotin can bind to avidin or streptavidin, and it can be eluted from avidin/streptavidin complex under milder conditions. PEG linker between azide and desthiobiotin offers higher water solubility, reduced steric hindrance and enhanced activity.
Physical Properties:
Off-white/white solid;
Soluble in aqeous solution, DMSO or DMF;
Reactive group: Azide (-N3);
Reactive to: Alkye, DBCO, etc.;
Storage Conditions:
Store at -20 0C. Desiccate. Avoid frequent thaw and freeze.
