北京中惠普氢空一体机HA-300

发表于2023年12月5日由上海金畔生物科技有限公司

北京中惠普氢空一体机HA-300

品牌中惠普|BCHP

型号 HA-300

商品详情

仪器特点：
一体机特点：不锈钢储气罐，三支不锈钢过滤器，内有微量氧脱除剂，气体纯度高；采用硅橡胶密封圈，含硫量低，对检测器无任何影响；结构紧凑，使用方便，全自动操作，安全可靠，可适用于任何厂家生产的任何型号的气相色谱仪。
氢气部分：自动防返碱，流量自动跟踪。
空气部分：高精度稳压，自动放水，具有完善的保护措施。

技术参数

氢气纯度氢气流量	99.999% 0-300ml/min
氢气压力	0.4MPa
空气流量空气压力	0-2000ml/min 0.4MPa
消耗功率	300W
外形尺寸	470×260×380(mm)
净重	28Kg

(E/Z)-HA155

发表于2023年12月3日由上海金畔生物科技有限公司

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

(E/Z)-HA155

(E/Z)-HA155 是一种有效的自分泌运动因子 autotaxin (ATX) I 型抑制剂。(E/Z)-HA155 可用于研究癌症、纤维性疾病、炎症、疼痛和血管生成。

(E/Z)-HA155 Chemical Structure

CAS No. : 1229652-22-5

规格		是否有货
100 mg		询价
250 mg		询价
500 mg		询价

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生物活性	(E/Z)-HA155 is a potent autotaxin (ATX) type I inhibitor. (E/Z)-HA155 can be used for researching cancer, fibrotic diseases, inflammation, pain and angiogenesis^[1].
IC₅₀ & Target	Autotaxin^[1]
分子量	463.29
Formula	C₂₄H₁₉BFNO₅S
CAS 号	1229652-22-5
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. Joncour A, et al. Discovery, Structure-Activity Relationship, and Binding Mode of an Imidazo[1,2-a]pyridine Series of Autotaxin Inhibitors [published correction appears in J Med Chem. 2018 May 10;61(9):4270]. J Med Chem. 2017;60(17):7371-7392.

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Fasudil Hydrochloride(Synonyms: 盐酸法舒地尔; HA-1077 Hydrochloride; AT-877 Hydrochloride)

发表于2023年10月11日由上海金畔生物科技有限公司

Fasudil Hydrochloride (Synonyms: 盐酸法舒地尔; HA-1077 Hydrochloride; AT-877 Hydrochloride) 纯度: 99.87%

Fasudil Hydrochloride (HA-1077 Hydrochloride; AT877 Hydrochloride) 是一种非特异性 RhoA/ROCK 抑制剂，对蛋白激酶也有抑制作用，ROCK1 的 K_i 值为 0.33 μM，ROCK2 和 PKA，PKC，PKG 的 IC₅₀ 值分别 0.158 μM 和 4.58 μM，12.30 μM，1.650 μM。Fasudil Hydrochloride 也是一种有效的 Ca²⁺ 通道拮抗剂和血管扩张剂。

Fasudil Hydrochloride Chemical Structure

CAS No. : 105628-07-7

规格	价格	是否有货
Free Sample (0.1-0.5 mg)		Apply now
10 mM * 1 mL in DMSO	￥605	In-stock
100 mg	￥550	In-stock
200 mg	￥770	In-stock
500 mg	￥1252	In-stock
1 g		询价
5 g		询价

* Please select Quantity before adding items.

Fasudil Hydrochloride 相关产品

•相关化合物库:

Drug Repurposing Compound Library Plus
FDA-Approved Drug Library Plus
FDA-Approved Drug Library Mini
Bioactive Compound Library Plus
Anti-Infection Compound Library
Cell Cycle/DNA Damage Compound Library
Epigenetics Compound Library
Kinase Inhibitor Library
Membrane Transporter/Ion Channel Compound Library
Neuronal Signaling Compound Library
Protein Tyrosine Kinase Compound Library
Stem Cell Signaling Compound Library
TGF-beta/Smad Compound Library
FDA-Approved Drug Library
Anti-Cancer Compound Library
Antiviral Compound Library
Autophagy Compound Library
Anti-Aging Compound Library
Drug Repurposing Compound Library
Reprogramming Compound Library
Oxygen Sensing Compound Library
Anti-COVID-19 Compound Library
NMPA-Approved Drug Library
Cytoskeleton Compound Library
Orally Active Compound Library
FDA Approved & Pharmacopeial Drug Library
Neuroprotective Compound Library
Rare Diseases Drug Library
Children’s Drug Library

生物活性

Fasudil Hydrochloride (HA-1077 Hydrochloride; AT877 Hydrochloride), is a nonspecific RhoA/ROCK inhibitor and also has inhibitory effect on protein kinases, with an K_i of 0.33 μM for ROCK1, IC₅₀s of 0.158 μM and 4.58 μM, 12.30 μM, 1.650 μM for ROCK2 and PKA, PKC, PKG, respectively^[1]. Fasudil Hydrochloride is also a potent Ca²⁺ channel antagonist and vasodilator^[2].

IC₅₀ & Target^[1]

p160ROCK

0.33 μM (Ki)

ROCK2

0.158 μM (IC₅₀)

PKA

4.58 μM (IC₅₀)

PKC

12.30 μM (IC₅₀)

PKG

1.65 μM (IC₅₀)

体外研究
(In Vitro)

Fasudil Hydrochloride (100 μM) inhibits cell spreading, the formation of stress fibers, and expression of α-SMA with concomitant suppression of cell growth in rat HSCs and human HSC-derived TWNT-4 cells^[4].
Fasudil Hydrochloride (50-100 μM; 24 hours) inhibits the LPA-induced phosphorylation of ERK1/2, JNK, and p38 detected by western blotting in rat HSCs and human HSC-derived TWNT-4 cells^[4].
Fasudil Hydrochloride (25-100 μM; 24 hours) suppresses transcription of collagen and TIMP, stimulates transcription of MMP-1 in human HSC-derived TWNT-4 cells^[4].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[4]

Cell Line:	Rat HSCs and human HSC-derived TWNT-4 cells
Concentration:	50 μM; 100 μM
Incubation Time:	24 hours
Result:	Suppressed the LPA-induced phosphorylation of ERK1/2, JNK and p38 MAPK by 60%, 70%,and 90%, respectively.

RT-PCR^[4]

Cell Line:	Rat HSCs and human HSC-derived TWNT-4 cells
Concentration:	25 μM; 50 μM; 100 μM 24 hours
Incubation Time:	24 hours
Result:	Reduced the expression of type I collagen, a-SMA, and TIMP-1.

体内研究
(In Vivo)

Fasudil (30 μg) increases CBF by 50% via intra-coronary injection to dogs. Fasudil (0.01, 0.03, 0.1 and 0.3 mg/kg, bolus, i.v.) decreases MBP and increases HR, VBF, CBF, RBF, and FBF. Fasudil (1.0 ng/mL) increases cardiac output. Fasudil via i.v. produces a significant fall in MBP, left ventricular systolic pressure and total peripheral resistance with an increase in HR and cardiac output, but without obvious effect on right atrial pressure, dP/dt or left ventricular minute work in dogs^[3]. Fasudil exhibits protectable effects on cardiovascular disease and reduces the activation of JNK and attenuates mitochondrial-nuclear translocation of AIF under ischemic injury^[6]. Fasudil (100 mg/kg/day, p.o.) significantly reduces incidence and mean maximum clinical score of EAE in SJL/J mice immunized with PLP p139-151. Fasudil inhibits the proliferative response of splenocytes to the antigen in mice. Fasudil decreases inflammation, demyelination, axonal loss and APP positivein spinal cord of Fasudil-treated mice via p.o. administration^[7].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

327.83

Formula

C₁₄H₁₈ClN₃O₂S

CAS 号

105628-07-7

中文名称

盐酸法舒地尔

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

H₂O : 55 mg/mL (167.77 mM; Need ultrasonic)

DMSO : ≥ 31 mg/mL (94.56 mM)

* “≥” means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.0504 mL	15.2518 mL	30.5036 mL
5 mM	0.6101 mL	3.0504 mL	6.1007 mL
10 mM	0.3050 mL	1.5252 mL	3.0504 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： PBS

Solubility: 100 mg/mL (305.04 mM); Clear solution; Need ultrasonic
2.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline

Solubility: ≥ 2.08 mg/mL (6.34 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (6.34 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
3.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.08 mg/mL (6.34 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (6.34 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
4.

请依序添加每种溶剂： 10% DMSO 90% corn oil

Solubility: ≥ 2.08 mg/mL (6.34 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (6.34 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在上海金畔生物科技有限公司网站选购。

参考文献

[1]. Chen M, et al. Fasudil and its analogs: a new powerful weapon in the long war against central nervous system disorders? Expert Opin Investig Drugs. 2013 Apr;22(4):537-50.

[2]. Huang XN, et al. The effects of fasudil on the permeability of the rat blood-brain barrier and blood-spinal cordbarrier following experimental autoimmune encephalomyelitis. J Neuroimmunol. 2011 Oct 28;239(1-2):61-7.

[3]. Uehata M, et al. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension. Nature. 1997 Oct 30;389(6654):990-4.

[4]. Fukushima M, et al. Fasudil hydrochloride hydrate, a Rho-kinase (ROCK) inhibitor, suppresses collagen production and enhances collagenase activity in hepatic stellate cells. Liver Int. 2005 Aug;25(4):829-38.

[5]. Corbin KD, et al. Choline metabolism provides novel insights into nonalcoholic fatty liver disease and its progression. Curr Opin Gastroenterol. 2012 Mar;28(2):159-65.

[6]. Zhang J, et al. Inhibition of the activity of Rho-kinase reduces cardiomyocyte apoptosis in heart ischemia/reperfusion via suppressing JNK-mediated AIF translocation. Clin Chim Acta. 2009 Mar;401(1-2):76-80.

[7]. Sun X, et al. The selective Rho-kinase inhibitor Fasudil is protective and therapeutic in experimental autoimmune encephalomyelitis. J Neuroimmunol. 2006 Nov;180(1-2):126-34. Epub 2006 Sep 22.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

HA15

发表于2023年10月10日由上海金畔生物科技有限公司

HA15 纯度: 99.62%

HA15 是一种高效特异性的内质网伴侣蛋白 BiP/GRP78/HSPA5 抑制剂，可抑制 BiP 的 ATP 酶活性，具有抗肿瘤活性。

HA15 Chemical Structure

CAS No. : 1609402-14-3

规格	价格	是否有货
Free Sample (0.1-0.5 mg)		Apply now
10 mM * 1 mL in DMSO	￥847	In-stock
5 mg	￥770	In-stock
10 mg	￥1100	In-stock
50 mg	￥3500	In-stock
100 mg	￥6000	In-stock
200 mg		询价
500 mg		询价

* Please select Quantity before adding items.

HA15 相关产品

•相关化合物库:

Bioactive Compound Library Plus
Apoptosis Compound Library
Cell Cycle/DNA Damage Compound Library
Metabolism/Protease Compound Library
Anti-Cancer Compound Library
Autophagy Compound Library
Anti-Aging Compound Library
Endoplasmic Reticulum Stress Compound Library
Cytoskeleton Compound Library

生物活性

HA15 is a potent and specific inhibitor of ER chaperone BiP/GRP78/HSPA5, inhibits the ATPase activity of BiP, with anti-cancerous activity^[1].

IC₅₀ & Target

BiP/GRP78/HSPA5^[1]

体外研究
(In Vitro)

HA15 (10 μM; 1-24 hours) induces an early endoplasmic reticulum stress (ER Stress)^[1].
HA15 (0-10μM; 24 hours) decreases melanoma cell viability in a dose-dependent manner compared with control conditions (DMSO), with an IC₅₀ of 1-2.5 μM in A375 cells^[1].
HA15 (1-10 μM; 24 hours) induces apoptosis in A375 cells^[1].
HA15 (1-24 μM; 24 hours) induces autophagy^[1].
HA15 (10 μM; 48 hours) has high efficiency in inducing cell death and ER stress in BRAF-inhibitor-resistant melanoma cells. And HA15 inhibits tumor growth through autophagic and apoptotic mechanisms initiated by ER stress^[1].
No deleterious effects on the viability of normal human melanocytes or human fibroblasts were observed with low or high doses of HA15^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	A375 cells
Concentration:	1 μM,2.5 μM,5 μM,7.5 μM,10 μM
Incubation Time:	24 hours
Result:	Decreased melanoma cell viability in a dose-dependent manner compared with control conditions (DMSO) in A375 cells.

Apoptosis Analysis^[1]

Cell Line:	A375 cells
Concentration:	1 μM, 5 μM, 10 μM
Incubation Time:	24 hours
Result:	Induces apoptosis.

Cell Autophagy Assay^[1]

Cell Line:	A375 cells
Concentration:	1 μM, 4 μM, 10 μM, 24 μM
Incubation Time:	24 hours
Result:	Increased LC3B-II expression after 1 hour and persisted after 24 hours, enhanced the expression level of Beclin 1, clearly be indicated that induces autophagy.

Western Blot Analysis^[1]

Cell Line:	A375 cells
Concentration:	10 μM
Incubation Time:	1 hour, 4 hours, 10 hours, 24 hours
Result:	Exhibited a rapid induction within 1 hour of the ER stress markers (phosphorylation of PERK and elF2α and a weak increase in ATF4 and CHOP expression)

体内研究
(In Vivo)

HA15 (0.7 mg/mouse/day; i.h.; over 2 weeks) inhibits melanoma tumor development in mice, induces no apparent toxicity and no change in their behavior, body mass, or liver mass, suggesting an absence of hepatomegaly^[1].
HA15 (0.7 mg/mouse; i.p.; 5 days/week) suppresses MPM tumor growth in vivo^[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	6-weeks female BALB/c nu/nu (nude) mice with A375 melanoma cells xenograft^[1]
Dosage:	0.7 mg/mouse/day
Administration:	Subcutaneous injection; over a period of 2 weeks
Result:	Attenuated the development of tumors.

Animal Model:	Mouse, NSG (NOD-scid IL2Rγnull)^[3]
Dosage:	0.7 mg/mouse
Administration:	Intraperitoneal injection, 5 days/week, for 5 weeks
Result:	Suppressed MPM tumor growth.

分子量

466.58

Formula

C₂₃H₂₂N₄O₃S₂

CAS 号

1609402-14-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : ≥ 50 mg/mL (107.16 mM)

* “≥” means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.1433 mL	10.7163 mL	21.4326 mL
5 mM	0.4287 mL	2.1433 mL	4.2865 mL
10 mM	0.2143 mL	1.0716 mL	2.1433 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline

Solubility: ≥ 2.5 mg/mL (5.36 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (5.36 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在上海金畔生物科技有限公司网站选购。

参考文献

[1]. Cerezo M et al. Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Cancer Cell. 2016 Jun 13;29(6):805-19.

[2]. Ruggiero C, et al. The GRP78/BiP inhibitor HA15 synergizes with mitotane action against adrenocortical carcinoma cells through convergent activation of ER stress pathways. Mol Cell Endocrinol. 2018 Oct 15;474:57-64.

[3]. Duo Xu, et al. Endoplasmic Reticulum Stress Signaling as a Therapeutic Target in Malignant Pleural Mesothelioma. Cancers (Basel). 2019 Oct; 11(10): 1502.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Fasudil(Synonyms: 法舒地尔; HA-1077; AT877)

发表于2023年9月19日由上海金畔生物科技有限公司

Fasudil (Synonyms: 法舒地尔; HA-1077; AT877)

Fasudil (HA-1077; AT877) 是一种非特异性 RhoA/ROCK 抑制剂，对蛋白激酶也有抑制作用，ROCK1 的 K_i 值为 0.33 μM，ROCK2 和 PKA，PKC，PKG 的 IC₅₀ 值分别 0.158 μM 和 4.58 μM，12.30 μM，1.650 μM。Fasudil 也是一种有效的 Ca²⁺ 通道拮抗剂和血管扩张剂。

Fasudil Chemical Structure

CAS No. : 103745-39-7

规格		是否有货
100 mg		询价
250 mg		询价
500 mg		询价

* Please select Quantity before adding items.

Fasudil 的其他形式现货产品:

Fasudil Hydrochloride

生物活性

Fasudil (HA-1077; AT877), is a nonspecific RhoA/ROCK inhibitor and also has inhibitory effect on protein kinases, with an K_i of 0.33 μM for ROCK1, IC₅₀s of 0.158 μM and 4.58 μM, 12.30 μM, 1.650 μM for ROCK2 and PKA, PKC, PKG, respectively^[1]. Fasudil is also a potent Ca2+channel antagonist and vasodilator^[2].

IC₅₀ & Target^[1]

p160ROCK

0.33 μM (Ki)

ROCK2

0.158 μM (IC₅₀)

PKA

4.58 μM (IC₅₀)

PKC

12.3 μM (IC₅₀)

PKG

1.65 μM (IC₅₀)

体外研究
(In Vitro)

Fasudil (100 μM) inhibits cell spreading, the formation of stress fibers, and expression of α-SMA with concomitant suppression of cell growth in rat HSCs and human HSC-derived TWNT-4 cells^[4].
Fasudil (50-100 μM; 24 hours) inhibits the LPA-induced phosphorylation of ERK1/2, JNK, and p38 detected by western blotting in rat HSCs and human HSC-derived TWNT-4 cells^[4].
Fasudil (25-100 μM; 24 hours) suppresses transcription of collagen and TIMP, stimulates transcription of MMP-1 in human HSC-derived TWNT-4 cells^[4].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[4]

Cell Line:	Rat HSCs and human HSC-derived TWNT-4 cells
Concentration:	50 μM; 100 μM
Incubation Time:	24 hours
Result:	Suppressed the LPA-induced phosphorylation of ERK1/2, JNK and p38 MAPK by 60%, 70%,and 90%, respectively.

RT-PCR^[4]

Cell Line:	Rat HSCs and human HSC-derived TWNT-4 cells
Concentration:	25 μM; 50 μM; 100 μM
Incubation Time:	24 hours
Result:	Reduced the expression of type I collagen, a-SMA, and TIMP-1.

体内研究
(In Vivo)

Fasudil (intravenous injection; 0.01, 0.03, 0.1 and 0.3 mg/kg) decreases MBP and increases HR, VBF, CBF, RBF, and FBF.
Fasudil (1.0 ng/mL) increases cardiac output. Fasudil via i.v. produces a significant fall in MBP, left ventricular systolic pressure and total peripheral resistance with an increase in HR and cardiac output, but without obvious effect on right atrial pressure, dP/dt or left ventricular minute work in dogs^[5].
Fasudil exhibits protectable effects on cardiovascular disease and reduces the activation of JNK and attenuates mitochondrial-nuclear translocation of AIF under ischemic injury^[6].
Fasudil (orally administration; 100 mg/kg/day) significantly reduces incidence and mean maximum clinical score of EAE in SJL/J mice immunized with PLP p139-151. Fasudil inhibits the proliferative response of splenocytes to the antigen in mice. Fasudil decreases inflammation, demyelination, axonal loss and APP positivein spinal cord of Fasudil-treated mice via p.o. administration^[7].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

291.37

Formula

C₁₄H₁₇N₃O₂S

CAS 号

103745-39-7

中文名称

法舒地尔

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献

[1]. Chen M, et al. Fasudil and its analogs: a new powerful weapon in the long war against central nervous system disorders? Expert Opin Investig Drugs. 2013 Apr;22(4):537-50.

[2]. Huang XN, et al. The effects of fasudil on the permeability of the rat blood-brain barrier and blood-spinal cordbarrier following experimental autoimmune encephalomyelitis. J Neuroimmunol. 2011 Oct 28;239(1-2):61-7.

[3]. Uehata M, et al. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension. Nature. 1997 Oct 30;389(6654):990-4.

[4]. Fukushima M, et al. Fasudil hydrochloride hydrate, a Rho-kinase (ROCK) inhibitor, suppresses collagen production and enhances collagenase activity in hepatic stellate cells. Liver Int. 2005 Aug;25(4):829-38.

[5]. Corbin KD, et al. Choline metabolism provides novel insights into nonalcoholic fatty liver disease and its progression. Curr Opin Gastroenterol. 2012 Mar;28(2):159-65.

[6]. Zhang J, et al. Inhibition of the activity of Rho-kinase reduces cardiomyocyte apoptosis in heart ischemia/reperfusion via suppressing JNK-mediated AIF translocation. Clin Chim Acta. 2009 Mar;401(1-2):76-80.

[7]. Sun X, et al. The selective Rho-kinase inhibitor Fasudil is protective and therapeutic in experimental autoimmune encephalomyelitis. J Neuroimmunol. 2006 Nov;180(1-2):126-34. Epub 2006 Sep 22.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

HA-100

发表于2023年9月7日由上海金畔生物科技有限公司

HA-100 纯度: 99.77%

HA-100 是一种有效的蛋白激酶抑制剂，抑制 PKG，PKA，PKC 和 MLC 激酶的 IC₅₀ 值分别为 4 μM、8 μM、12 μM 和 240 μM。HA-100 也用作 ROCK 抑制剂。

HA-100 Chemical Structure

CAS No. : 84468-24-6

规格	价格	是否有货
Free Sample (0.1-0.5 mg)		Apply now
10 mM * 1 mL in DMSO	￥847	In-stock
5 mg	￥770	In-stock
10 mg	￥1300	In-stock
25 mg	￥2800	In-stock
50 mg	￥5000	In-stock
100 mg	￥9000	In-stock
200 mg		询价
500 mg		询价

* Please select Quantity before adding items.

HA-100 相关产品

•相关化合物库:

Bioactive Compound Library Plus
Cell Cycle/DNA Damage Compound Library
Epigenetics Compound Library
Kinase Inhibitor Library
Protein Tyrosine Kinase Compound Library
Stem Cell Signaling Compound Library
TGF-beta/Smad Compound Library
Anti-Cancer Compound Library
Anti-Aging Compound Library
Reprogramming Compound Library
Oxygen Sensing Compound Library
Cytoskeleton Compound Library

生物活性

HA-100 is a potent protein kinase inhibitor, with IC₅₀s of 4 μM, 8 μM, 12 μM and 240 μM for cGMP-dependent protein kinase (PKG), cAMP-dependent protein kinase (PKA), protein kinase C (PKC) and MLC-kinase, respectively. HA-100 also used as a ROCK inhibitor^[1]^[2].

IC₅₀ & Target^[1]

PKC

12 μM (IC₅₀)

PKG

4 μM (IC₅₀)

PKA

8 μM (IC₅₀)

MLCK

240 μM (IC₅₀)

PKC

6.5 μM (Ki)

MLCK

61 μM (Ki)

体外研究
(In Vitro)

HA-100 inhibits MLC-kinase and PKC competitively with respect to ATP, with K_is of 61 and 6.5 μM, respectively^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

277.34

Formula

C₁₃H₁₅N₃O₂S

CAS 号

84468-24-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 150 mg/mL (540.85 mM; Need ultrasonic and warming)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.6057 mL	18.0284 mL	36.0568 mL
5 mM	0.7211 mL	3.6057 mL	7.2114 mL
10 mM	0.3606 mL	1.8028 mL	3.6057 mL

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline

Solubility: ≥ 2.5 mg/mL (9.01 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.01 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (9.01 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.01 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil

Solubility: ≥ 2.5 mg/mL (9.01 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.01 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在上海金畔生物科技有限公司网站选购。

参考文献

[1]. Hagiwara M, et al. Selective modulation of calcium-dependent myosin phosphorylation by novel protein kinase inhibitors, isoquinolinesulfonamide derivatives. Mol Pharmacol. 1987 Jul;32(1):7-12.

[2]. Yu J, et, al. Efficient feeder-free episomal reprogramming with small molecules. PLoS One. 2011 Mar 1;6(3):e17557.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

人血凝素（HA）ELISA试剂盒免费代测BS-1133

发表于2023年8月26日由上海金畔生物科技有限公司

人血凝素（HA）ELISA试剂盒免费代测

产品型号：BS-1133
简要描述：人血凝素（HA）ELISA试剂盒免费代测金畔生物公司供应：ELISA试剂盒,荧光定量PCR耗材，移液器吸嘴，微量离心管，进口冻存管，细胞培养皿，培养板，培养瓶，吸头，仪器及手套，色谱耗材，针头过滤器。

产品咨询在线客服

产品简介

人血凝素(HA)ELISA试剂盒免费代测金畔生物公司供应：ELISA试剂盒,血清，荧光定量PCR耗材，移液器吸嘴，微量离心管，进口冻存管，细胞培养皿，培养板，培养瓶，吸头，仪器及手套，色谱耗材，针头过滤器。

规格：96T/48T

BS-1133 人血凝素(HA)ELISA试剂盒

检测种属：人、大小鼠、豚鼠、兔子、猪、犬、牛羊、鸡鸭、猴ELISA试剂盒等种属。

保存条件及有效期：

1、试剂盒保存：2-8℃。

2、有效期：6个月

标记物：血清、血浆、组织匀浆等

【测试种属】犬、大小鼠、人、豚鼠、兔子、牛羊、猪、鸡鸭elisa试剂盒等种属
【储存方式】：2-8℃
【检测目的】用于测定血清，血浆及相关液体等样本。例如适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本。
【用途】科研实验，不用于临床诊断。

人血凝素(HA)ELISA试剂盒免费代测

样本处理及要求：

1. 血清：室温血液自然凝固10-20分钟，离心20分钟左右(2000-3000转/分)。仔细收集上清，保存过程中如出现沉淀，应再次离心。

2. 血浆：应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂，混合10-20分钟后，离心20分钟左右(2000-3000转/分)。仔细收集上清，保存过程中如有沉淀形成，应该再次离心。

3. 尿液：用无菌管收集，离心20分钟左右(2000-3000转/分)。仔细收集上清，保存过程中如有沉淀形成，应再次离心。胸腹水、脑脊液参照实行。

4. 细胞培养上清：检测分泌性的成份时，用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时，用PBS(PH7.2-7.4)稀释细胞悬液，细胞浓度达到100万/ml左右。通过反复冻融，以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成，应再次离心。

5. 组织标本：切割标本后，称取重量。加入一定量的PBS，PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4)，用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测，其余冷冻备用。

6. 标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融.

7. 不能检测含NaN3的样品，因NaN3抑制辣根过氧化物酶的(HRP)活性。

人血凝素(HA)ELISA检测试剂盒免费代测操作步骤：

人血凝素（HA）ELISA试剂盒免费代测BS-1133

检测试剂盒组成：
试剂盒组成48 孔配置96 孔配置保存
说明书1 份1 份
封板膜2 片(48)2 片(96)
密封袋1 个1 个
酶标包被板1×481×962-8℃保存
标准品：540μg/L0.5ml×1 瓶0.5ml×1 瓶2-8℃保存
标准品稀释液1.5ml×1 瓶1.5ml×1 瓶2-8℃保存
酶标试剂3 ml×1 瓶6 ml×1 瓶2-8℃保存
样品稀释液3 ml×1 瓶6 ml×1 瓶2-8℃保存
显色剂 A 液3 ml×1 瓶6 ml×1 瓶2-8℃保存
显色剂 B 液3 ml×1 瓶6 ml×1 瓶2-8℃保存
终止液3 ml×1 瓶6 ml×1 瓶2-8℃保存
浓缩洗涤液(20ml×20 倍)×1 瓶(20ml×30 倍)×1 瓶2-8℃保存

一次性滤芯吸头|无菌低吸附吸嘴|微量吸头

加长吸头带滤芯吸头盒装无菌低吸附吸嘴

吸头进口滤芯吸头,移液枪专用吸嘴进口国产

Tips100-1300ul进口加长吸嘴

Tips1-200ul进口加长吸嘴,黄色

Tip 1-200ul, 进口加长吸嘴，无色

Tip 1-300ul, 进口加长吸嘴，无色

Tip 100-1300ul进口加长吸嘴,无色,带滤芯,袋装非灭菌

带滤芯无菌吸头10盒/5包/箱

100ml环境取样瓶无硫代硫酸钠灭菌

1000ml无菌瓶方瓶

Pipettor Stands移液枪托板，白色展板，6孔

Tip 1-200ul国产加长吸嘴,无色, 带滤芯, 盒装

96孔PCR管架

带滤芯吸头移液器吸头/200ul进口加长吸嘴

产品用途：可用于科研实验,不用于临床治疗！

HA130

发表于2023年8月11日由上海金畔生物科技有限公司

HA130 纯度: 99.43%

HA130 是一种选择性的 autotaxin (ATX) 抑制剂，IC₅₀ 为 28 nM。

HA130 Chemical Structure

CAS No. : 1229652-21-4

规格	价格	是否有货
Free Sample (0.1-0.5 mg)		Apply now
10 mM * 1 mL in DMSO	￥610	In-stock
5 mg	￥600	In-stock
10 mg	￥1000	In-stock
50 mg	￥3000	In-stock
100 mg		询价
200 mg		询价

* Please select Quantity before adding items.

HA130 相关产品

•相关化合物库:

Covalent Screening Library Plus
Bioactive Compound Library Plus
Metabolism/Protease Compound Library
Anti-Cancer Compound Library
Covalent Screening Library
Anti-Alzheimer’s Disease Compound Library
Neurodegenerative Disease-related Compound Library
Anti-Colorectal Cancer Compound Library

生物活性

HA130 is a selective autotaxin (ATX) inhibitor with an IC₅₀ of 28 nM.

IC₅₀ & Target^[1]

Autotaxin

28 nM (IC₅₀)

体外研究
(In Vitro)

HA130 completely blocks the ability of ATX to promote TEM (transendothelial migration). HA130 at 0.3 μM completely ablates the activity of ATX on TK1 uropod formation^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

HA130 slows T cell migration across lymph node HEVs. HA130 decreases the “outside HEVs/inside HEVs” ratio by 3-4-fold compared to vehicle-treated animals vehicle^[1].
The s.c. administration of HA130 induces marked lymphocyte accumulation within the endothelial cell (EC) and sub-EC layers of HEVs in draining lymph nodes (LNs)^[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

463.29

Formula

C₂₄H₁₉BFNO₅S

CAS 号

1229652-21-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : ≥ 39 mg/mL (84.18 mM)

* “≥” means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.1585 mL	10.7924 mL	21.5848 mL
5 mM	0.4317 mL	2.1585 mL	4.3170 mL
10 mM	0.2158 mL	1.0792 mL	2.1585 mL

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline

Solubility: ≥ 2.5 mg/mL (5.40 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (5.40 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% corn oil

Solubility: ≥ 2.5 mg/mL (5.40 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (5.40 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在上海金畔生物科技有限公司网站选购。

参考文献

[1]. Zhang Y, et al. Autotaxin through lysophosphatidic acid stimulates polarization, motility, and transendothelial migration of naive T cells. J Immunol. 2012 Oct 15;189(8):3914-3924.

[2]. Bai Z, et al. Constitutive lymphocyte transmigration across the basal lamina of high endothelial venules is regulated by theautotaxin/lysophosphatidic acid axis. J Immunol. 2013 Mar 1;190(5):2036-2048.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

美国Labnet孵育杂交箱H1200A-230V

发表于2023年6月23日由上海金畔生物科技有限公司

【简单介绍】

美国Labnet孵育杂交箱H1200A-230V，很好的温度控制系统可保证实验结果的稳定性，速度可调、温度和转轴可调，适用于35mm瓶和50ml管，内部为带插座的耐用不锈钢结构，摇动托盘和附带支架可选。

进口，！

【详细说明】

美国Labnet孵育杂交箱H1200A-230V

产品介绍：

ProtBot 12可一次放置12个杂交管，能满足不同规模实验室的需求。

ProBot系列杂交炉的转轴和温育系统可以提供良好的实验环境，使得实验结果重复性好，背景低。在数字温控的基础上，机械对流装置保证了杂交炉内环境的均一性。一体化的观察区域可以在不扰动温度的情况下实现样品的可视化。同时样品转动的强度也可以改变转轴的转动速度兼幅度来得以调节。

内部为抗腐蚀的镜面不绣钢转轴。杂交炉将配带一个同时携有直径35mm长50ml的可重复使用的玻璃管的转轴,两个大瓶子，以及一卷杂交膜。可选配件有相应的摇床平台和其他规格的转轴。

产品优势：

准确的温度控制系统可保证实验结果的稳定性
速度可调、温度和转轴可调，适用于35mm瓶和50ml管
内部为带插座的耐用不锈钢结构
摇动托盘和附带支架可选

美国Labnet孵育杂交箱H1200A-230V

参数规格：

瓶子容量	12大/24小
转速	4-20rpm
温度范围	5 -80 ℃
温度控制精度	0.1 ℃ / 0.5 ℃
温度控制	电脑控制
外体积	48.344.549.5cm
内体积	36.827.934.3cm
重量	26.8Kg
电压	230V

货号	详细信息
H1200A-230V	ProBlot 12 杂交系统；附赠:2 个瓶子300×35mm， 1 包筛网
H1200-RA	ProBlot 托盘
H1212-40VA	ProBlot 架子，试管（ 12×50ml ）垂直放置
H1204 -80A	ProBlot 架子，试管（ 4× 70mm 直径）垂直放置
H1264-VA	ProBlot 64×1.5ml 或 32×15ml 架子，试管垂直放置
H1264-HA	ProBlot 64×1.5ml 或 32×15ml 架子，试管水平放置

赛默飞Alfa aesar官网

赛默飞Acros试剂中国官网 Alfa aesar阿法埃莎中国代理商

标签归档：ha

北京中惠普氢空一体机HA-300

(E/Z)-HA155

Fasudil Hydrochloride(Synonyms: 盐酸法舒地尔; HA-1077 Hydrochloride; AT-877 Hydrochloride)

Fasudil Hydrochloride 相关产品

•相关化合物库:

HA15

HA15 相关产品

•相关化合物库:

Fasudil(Synonyms: 法舒地尔; HA-1077; AT877)

HA-100

HA-100 相关产品

•相关化合物库:

人血凝素（HA）ELISA试剂盒免费代测BS-1133

人血凝素（HA）ELISA试剂盒免费代测

HA130

HA130 相关产品

•相关化合物库:

美国Labnet孵育杂交箱H1200A-230V