Methotrexate (Amethopterin) monohydrate, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis. Methotrexate monohydrate, also an immunosuppressant and antineoplastic agent, is used for the research of rheumatoid arthritis and a number of different cancers (such as acute lymphoblastic leukemia)[1][2][3].
IC50 & Target
Traditional Cytotoxic Agents
体内研究 (In Vivo)
Methotrexate (Amethopterin) monohydrate reduces thymus and spleen indices of mice. Methotrexate monohydrate markedly decreases white blood cells, thymic and splenic lymphocytes at dose ≥5 mg/kg. However, there is a significant difference between the treatment plus control group and the model group (p<0.01). The combination of grape seed proanthocyanidins and Siberian ginseng eleutherosides obviously diminishes the effects of Methotrexate monohydrate exposure on indices of thymus and spleens in mice[2]. Methotrexate (MTX) monohydrate (2 mg/kg; i.p.; once in a week for 5 weeks) is effective in Freund’s complete adjuvant-induced arthritis. The combination of Methotrexate monohydrate (1 mg/kg; i.p.; once in a week for 5 weeks) and Curcumin (30 mg/kg and 100 mg/kg, thrice a week for 5 weeks; i.p.) shows a significant anti-arthritic action and protection from hematological toxicity[4].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
472.45
Formula
C20H24N8O6
CAS 号
6745-93-3
中文名称
甲氨蝶呤一水合物;氨甲蝶呤一水合物;氨甲叶酸一水合物
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Tian H, et al. Understanding the mechanisms of action of methotrexate: implications for the treatment of rheumatoid arthritis. Bull NYU Hosp Jt Dis. 2007;65(3):168-73.
[2]. Swierkot J, et al. Methotrexate in rheumatoid arthritis. Pharmacol Rep. 2006 Jul-Aug;58(4):473-92.
[3]. Ehab Tousson, et al. The Effect of L-carnitine on Amethopterin-induced Toxicity in Rat Large Intestine.
[4]. Banji D, et al. Evaluation of the concomitant use of methotrexate and curcumin on Freund’s complete adjuvant-induced arthritis and hematological indices in rats. Indian J Pharmacol. 2011;43(5):546-550.
Methotrexate PEG derivatives are a class of bioactive PEGs that can be used to for drug discovery or bioassay development. Methotrexate, also called MTX, amethopterin, is an antimetabolite and antifolate drug. It is used in treatment of cancer, autoimmune diseases, ectopic pregnancy, and for the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Pegylated methotrexate provides better water solubility. Nanocs can provide other methotrexate PEG derivatives based on customer request.
Physical Properties:
Yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, desiccate. Avoid frequent thaw and freeze.
Methotrexate PEG derivatives are a class of bioactive PEGs that can be used to for drug discovery or bioassay development. Methotrexate, also called MTX, amethopterin, is an antimetabolite and antifolate drug. It is used in treatment of cancer, autoimmune diseases, ectopic pregnancy, and for the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Pegylated methotrexate provides better water solubility. Nanocs can provide other methotrexate PEG derivatives based on customer request.
Physical Properties:
Yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, desiccate. Avoid frequent thaw and freeze.
Methotrexate PEG derivatives are a class of bioactive PEGs that can be used to for drug discovery or bioassay development. Methotrexate, also called MTX, amethopterin, is an antimetabolite and antifolate drug. It is used in treatment of cancer, autoimmune diseases, ectopic pregnancy, and for the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Pegylated methotrexate provides better water solubility. Nanocs can provide other methotrexate PEG derivatives based on customer request.
Physical Properties:
Yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, desiccate. Avoid frequent thaw and freeze.
Methotrexate PEG derivatives are a class of bioactive PEGs that can be used to for drug discovery or bioassay development. Methotrexate, also called MTX, amethopterin, is an antimetabolite and antifolate drug. It is used in treatment of cancer, autoimmune diseases, ectopic pregnancy, and for the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Pegylated methotrexate provides better water solubility. Nanocs can provide other methotrexate PEG derivatives based on customer request.
Physical Properties:
Yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, desiccate. Avoid frequent thaw and freeze.
Methotrexate PEG DSPE is one of Nanocs bioactive phospholipid PEG derivatives that can be used to for drug discovery or bioassay development. Methotrexate, also called MTX, amethopterin, is an antimetabolite and antifolate drug. It is used in treatment of cancer, autoimmune diseases, ectopic pregnancy, and for the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Pegylated methotrexate provides better water solubility. Nanocs can provide other methotrexate PEG derivatives based on customer request.
Physical Properties:
Yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C. Desiccate. Avoid frequent thaw and freeze.
Methotrexate (Amethopterin) 是一种抗代谢 (antimetabolite) 和抗叶酸剂 (antifolate),可抑制二氢叶酸还原酶,从而防止叶酸转化为四氢叶酸并抑制 DNA 合成。Methotrexate也是一种免疫抑制剂和抗肿瘤剂,用于类风湿关节炎和研究多种癌症 (如急性淋巴细胞白血病)。
Methotrexate Chemical Structure
CAS No. : 59-05-2
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Free Sample (0.1-0.5 mg)
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10 mM * 1 mL in DMSO
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¥600
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Methotrexate 相关产品
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生物活性
Methotrexate (Amethopterin), an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis. Methotrexate, also an immunosuppressant and antineoplastic agent, is used for the research of rheumatoid arthritis and a number of different cancers (such as acute lymphoblastic leukemia)[1][2][3].
IC50 & Target
Traditional Cytotoxic Agents
体内研究 (In Vivo)
Methotrexate (Amethopterin) reduces thymus and spleen indices of mice. Methotrexate markedly decreases white blood cells, thymic and splenic lymphocytes at dose ≥5 mg/kg. However, there is a significant difference between the treatment plus control group and the model group (p<0.01). The combination of grape seed proanthocyanidins and Siberian ginseng eleutherosides obviously diminishes the effects of Methotrexate exposure on indices of thymus and spleens in mice[2]. Methotrexate (MTX) (2 mg/kg; i.p.; once in a week for 5 weeks) is effective in Freund’s complete adjuvant-induced arthritis. The combination of Methotrexate (1 mg/kg; i.p.; once in a week for 5 weeks) and Curcumin (30 mg/kg and 100 mg/kg, thrice a week for 5 weeks; i.p.) shows a significant anti-arthritic action and protection from hematological toxicity[4].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量
454.44
Formula
C20H22N8O5
CAS 号
59-05-2
中文名称
甲氨蝶呤;氨甲蝶呤;氨甲叶酸
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Tian H, et al. Understanding the mechanisms of action of methotrexate: implications for the treatment of rheumatoid arthritis. Bull NYU Hosp Jt Dis. 2007;65(3):168-73.
[2]. Swierkot J, et al. Methotrexate in rheumatoid arthritis. Pharmacol Rep. 2006 Jul-Aug;58(4):473-92.
[3]. Ehab Tousson, et al. The Effect of L-carnitine on Amethopterin-induced Toxicity in Rat Large Intestine.
[4]. Banji D, et al. Evaluation of the concomitant use of methotrexate and curcumin on Freund’s complete adjuvant-induced arthritis and hematological indices in rats. Indian J Pharmacol. 2011;43(5):546-550.
Cell Assay [1]
Each cell line is studied in growth inhibition experiments using 96-well microtiter plates. As antifols are schedule dependent, preliminary experiments are aimed at defining the longest duration of exposure that would allow for continuous logarithmic phase growth of cells without changing of the culture media while maintaining a linear relationship between SRB optical density and cell number. Twenty-four hours after cell plating, the cell lines are exposed to the antifol for 120 h (three replicates per experiment). To ensure that a complete sigmoidal survival-concentration curve could be observed, the following drug concentrations are studied: Methotrexate (0.002-5 μM), AMT (0.0001-1 μM), PXD (0.0003-10 μM), TLX (0.0002-0.5 μM). Experiments are repeated at least twice[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration [2]
Mice[2] The combination of bioactive phytochemicals is administered one week prior to the Methotrexate exposure. Treatment group I: mice are given a combination of green tea polyphenols and eleutherosides from Siberian ginseng (0.2 mL/10 g, i.g. once daily) for 15 days, and a single dose of Methotrexate (2 mg/kg, i.p. once daily) is added on the 8th day. Treatment group II: mice are given a combination of grape seed proanthocyanidins and eleutherosides from Siberian ginseng for 15 days, and Methotrexate is administered on the 8th day in a similar manner. Model group: animals received distilled water instead of bioactive phytochemicals combinations for 15 days and the same Methotrexate protocol applied to this group on the 8th day. Control group: mice are given distilled water through 15 days and physiological saline instead of Methotrexate is administered on the 8th day in a similar manner. Twelve hours after the final doses, the animals are euthanized by cervical dislocation.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献
[1]. Tian H, et al. Understanding the mechanisms of action of methotrexate: implications for the treatment of rheumatoid arthritis. Bull NYU Hosp Jt Dis. 2007;65(3):168-73.
[2]. Swierkot J, et al. Methotrexate in rheumatoid arthritis. Pharmacol Rep. 2006 Jul-Aug;58(4):473-92.
[3]. Ehab Tousson, et al. The Effect of L-carnitine on Amethopterin-induced Toxicity in Rat Large Intestine.
[4]. Banji D, et al. Evaluation of the concomitant use of methotrexate and curcumin on Freund’s complete adjuvant-induced arthritis and hematological indices in rats. Indian J Pharmacol. 2011;43(5):546-550.
Methotrexate-d3 (Amethopterin-d3) is the deuterium labeled Methotrexate. Methotrexate (Amethopterin), an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis. Methotrexate, also an immunosuppressant and antineoplastic agent, is used for the research of rheumatoid arthritis and a number of different cancers (such as acute lymphoblastic leukemia)[1][2][3].
体外研究 (In Vitro)
Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
457.46
Formula
C20H19D3N8O5
CAS 号
432545-63-6
中文名称
甲氨蝶呤 d3
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-20°C
3 years
In solvent
-80°C
6 months
-20°C
1 month
参考文献
[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019;53(2):211-216.
[2]. Tian H, et al. Understanding the mechanisms of action of methotrexate: implications for the treatment of rheumatoid arthritis. Bull NYU Hosp Jt Dis. 2007;65(3):168-73.
[3]. Swierkot J, et al. Methotrexate in rheumatoid arthritis. Pharmacol Rep. 2006 Jul-Aug;58(4):473-92.
[4]. Ehab Tousson, et al. The Effect of L-carnitine on Amethopterin-induced Toxicity in Rat Large Intestine.
[5]. Banji D, et al. Evaluation of the concomitant use of methotrexate and curcumin on Freund’s complete adjuvant-induced arthritis and hematological indices in rats. Indian J Pharmacol. 2011;43(5):546-550.
Toxins for Antibody-Drug Conjugate Research Library
Anti-Cancer Compound Library
Anti-Aging Compound Library
Drug Repurposing Compound Library
Orally Active Compound Library
FDA Approved & Pharmacopeial Drug Library
Drug-Induced Liver Injury (DILI) Compound Library
Anti-Blood Cancer Compound Library
Anti-Cancer Metabolism Compound Library
Rare Diseases Drug Library
Children’s Drug Library
生物活性
Methotrexate (Amethopterin) disodium, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis. Methotrexate disodium, also an immunosuppressant and antineoplastic agent, is used for the research of rheumatoid arthritis and a number of different cancers (such as acute lymphoblastic leukemia)[1][2][3].
IC50 & Target
Traditional Cytotoxic Agents
体内研究 (In Vivo)
Methotrexate (Amethopterin) disodium reduces thymus and spleen indices of mice. Methotrexate disodium markedly decreases white blood cells, thymic and splenic lymphocytes at dose ≥5 mg/kg. However, there is a significant difference between the treatment plus control group and the model group (p<0.01). The combination of grape seed proanthocyanidins and Siberian ginseng eleutherosides obviously diminishes the effects of Methotrexate exposure on indices of thymus and spleens in mice[2]. Methotrexate (MTX) disodium (2 mg/kg; i.p.; once in a week for 5 weeks) is effective in Freund’s complete adjuvant-induced arthritis. The combination of Methotrexate disodium (1 mg/kg; i.p.; once in a week for 5 weeks) and Curcumin (30 mg/kg and 100 mg/kg, thrice a week for 5 weeks; i.p.) shows a significant anti-arthritic action and protection from hematological toxicity[4].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量
498.40
Formula
C20H20N8Na2O5
CAS 号
7413-34-5
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4°C, sealed storage, away from moisture and light
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:
H2O : 50 mg/mL (100.32 mM; Need ultrasonic)
DMSO : 5 mg/mL (10.03 mM; ultrasonic and warming and adjust pH to 5 with HCl and heat to 60°C)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
2.0064 mL
10.0321 mL
20.0642 mL
5 mM
0.4013 mL
2.0064 mL
4.0128 mL
10 mM
0.2006 mL
1.0032 mL
2.0064 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
参考文献
[1]. Tian H, et al. Understanding the mechanisms of action of methotrexate: implications for the treatment of rheumatoid arthritis. Bull NYU Hosp Jt Dis. 2007;65(3):168-73.
[2]. Swierkot J, et al. Methotrexate in rheumatoid arthritis. Pharmacol Rep. 2006 Jul-Aug;58(4):473-92.
[3]. Swierkot J, et al. Methotrexate in rheumatoid arthritis. Pharmacol Rep. 2006 Jul-Aug;58(4):473-92.
[4]. Banji D, et al. Evaluation of the concomitant use of methotrexate and curcumin on Freund’s complete adjuvant-induced arthritis and hematological indices in rats. Indian J Pharmacol. 2011;43(5):546-550.
Methotrexate PEG derivatives are a class of bioactive PEGs that can be used to for drμg discovery or bioassay development. Methotrexate, also called MTX, amethopterin, is an antimetabolite and antifolate drμg. It is used in treatment of cancer, autoimmune diseases, ectopic pregnancy, and for the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Pegylated methotrexate provides better water solubility. Nanocs can provide other methotrexate PEG derivatives based on customer request.
Methotrexate PEG derivatives are a class of bioactive PEGs that can be used to for drug discovery or bioassay development. Methotrexate, also called MTX, amethopterin, is an antimetabolite and antifolate drug. It is used in treatment of cancer, autoimmune diseases, ectopic pregnancy, and for the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Pegylated methotrexate provides better water solubility. Nanocs can provide other methotrexate PEG derivatives based on customer request.
相关属性
溶解性
Yellow solid or viscous liquid depends on molecule weight;Soluble in regular aqeous solution as well as most organic solvents;
Methotrexate PEG derivatives are a class of bioactive PEGs that can be used to for drμg discovery or bioassay development. Methotrexate, also called MTX, amethopterin, is an antimetabolite and antifolate drμg. It is used in treatment of cancer, autoimmune diseases, ectopic pregnancy, and for the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Pegylated methotrexate provides better water solubility. Nanocs can provide other methotrexate PEG derivatives based on customer request.
Methotrexate PEG derivatives are a class of bioactive PEGs that can be used to for drug discovery or bioassay development. Methotrexate, also called MTX, amethopterin, is an antimetabolite and antifolate drug. It is used in treatment of cancer, autoimmune diseases, ectopic pregnancy, and for the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Pegylated methotrexate provides better water solubility. Nanocs can provide other methotrexate PEG derivatives based on customer request.
相关属性
溶解性
Yellow solid or viscous liquid depends on molecule weight;Soluble in regular aqeous solution as well as most organic solvents;
Methotrexate PEG derivatives are a class of bioactive PEGs that can be used to for drμg discovery or bioassay development. Methotrexate, also called MTX, amethopterin, is an antimetabolite and antifolate drμg. It is used in treatment of cancer, autoimmune diseases, ectopic pregnancy, and for the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Pegylated methotrexate provides better water solubility. Nanocs can provide other methotrexate PEG derivatives based on customer request.
Methotrexate PEG derivatives are a class of bioactive PEGs that can be used to for drug discovery or bioassay development. Methotrexate, also called MTX, amethopterin, is an antimetabolite and antifolate drug. It is used in treatment of cancer, autoimmune diseases, ectopic pregnancy, and for the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Pegylated methotrexate provides better water solubility. Nanocs can provide other methotrexate PEG derivatives based on customer request.
相关属性
溶解性
Yellow solid or viscous liquid depends on molecule weight;Soluble in regular aqeous solution as well as most organic solvents;