Peptide aldehyde that corresponds to one of the cleavage sites of the inactive 32 kD caspase-3 precursor (amino acids 172-175). In a concentration range of 0.5 碌M, it blocks the formation of the p17 subunit and concomitantly induces the accumulation of the 32kD precursor.
溶解度
分子量
675.7
化学式
C31H41N5O12
存储条件
Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents
Figures
Reference
N.A. Thornberry et al., J. Biol. Chem. , 272, 17907 (1997) Z. Han et al., J. Biol. Chem., 272, 13432 (1997)
Protease-Activated Receptor-1, PAR-1 Agonist TFA is a selective proteinase-activated receptor1 (PAR-1) agonist peptide. Protease-Activated Receptor-1, PAR-1 Agonist TFA corresponds to PAR1 tethered ligand and which can selectively mimic theactions of thrombin via this receptor[1][2].
体外研究 (In Vitro)
Protease-Activated Receptor-1, PAR-1 Agonist induces activation of protein kinase C isoenzymes alpha and epsilon in human HT-29 colon carcinoma cells expressing PAR1 endogeneously. On the cellular level, Protease-Activated Receptor-1, PAR-1 Agonist and thrombin prompted HT-29 cell migration and matrix adhesion by a PKCepsilon-dependent mechanism as concluded because of the inhibition of PAR1-mediated effects by the PKC inhibitor bisindolylmaleimide I and the PKCepsilon translocation inhibitory peptide EAVSLKPT but not by the PKC inhibitor Gö 6976[2].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
876.92
Formula
C37H59F3N10O11
Sequence
Thr-Phe-Leu-Leu-Arg-Asn
Sequence Shortening
TFLLRN
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Stefanie Gödecke, et al. Thrombin-induced ATP release from human umbilical vein endothelial cells. Am J Physiol Cell Physiol. 2012 Mar 15;302(6):C915-23.
[2]. Heider I, et al. PAR1-type thrombin receptor stimulates migration and matrix adhesion of human colon carcinoma cells by a PKCepsilon-dependent mechanism. Oncol Res. 2004;14(10):475-482.
Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents
Figures
Reference
Lin, C. et al. J. Biol. Chem. 10, 1074 (2004); Kakiuchi, N. et al. J. Virol. Methods 80, 77 (1999); Liu, Y. et al. Anal. Biochem. 267, 331 (1999); Taliani, M. et al. Anal. Biochem. 240, 60 (1996).
Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents
Figures
Reference
Lin, C. et al. J. Biol. Chem. 10, 1074 (2004) Kakiuchi, N. et al. J. Virol. Methods 80, 77 (1999) Liu, Y. et al. Anal. Biochem. 267, 331 (1999) Taliani, M. et al. Anal. Biochem. 240, 60 (1996)
This peptide inhibited activation of PAR-2 by trypsin in PAR-2 receptor expressing KNRK cells. Half-maximal inhibition of calcium signaling was observed at about 50 碌M. In contrast, the activation of PAR-2 by SLIGRL-NH2 (PAR-2 (1-6) amide (mouse, rat)) was not inhibited by this peptide. Selective Antagonist for PAR2 Agonist. This peptide blocks trypsin but not SLIGRL-NH2 activation of PAR2 in receptor-expressing KNRK cells.
溶解度
分子量
782.9
化学式
C37H54N10O9
存储条件
Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents
Figures
Reference
Z.Suo et al., J. Biol. Chem., 278, 37681 (2003) P.J.Yu et al., Oncogene, 27, 2594 (2008) F.Bock et al., Proc. Natl. Acad. Sci. USA, 110, 648 (2013)
The peptide is a protease-activated receptor-2 (PAR2) selective agonist.
溶解度
分子量
657.81
化学式
C29H55N9O8
存储条件
Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents
Figures
Reference
R.J.Santulli et al., Proc. Natl. Acad. Sci. USA, 92, 9151 (1995) A. Bhattacharya et al., J. Pharmacol. Exp. Ther., 297, 573 (2001) B. Al-Ani, et al., J. of Physiol. Pharmacol., 73, 1203 (1995)
This peptide mimicks the putative tethered ligand of human PAR-3 (protease activated receptor 3). PAR3 Tethered Ligand (Human) / Activates PAR1 and PAR2
溶解度
分子量
656.83
化学式
C29H56N10O7
存储条件
Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents
Figures
Reference
B.al-Ani et al., Can. J. Physiol. Pharmacol., 73, 1203 (1995) M.D.Hollenberg et al., Mol. Pharmacol., 49, 229 (1996) M.Saiffeddine et al., Br. J. Pharmacol., 118, 521 (1996) N.Vergnolle, J. Immunol., 163, 5064 (1999) B.Al-Ani et al., Br. J. Pharmacol., 128, 1105 (1999) E.R.Sharlow et al., J. Cell Sci., 113, 3093 (2000)
This peptide is a human PAR-3-derived tethered ligand, which does not activate PAR-3 but rather activates PAR-1 and PAR-2, either in Jurkat or in other PAR-expressing cells. PAR3 Tethered Ligand (Human) / Activates PAR1 and PAR2 This peptide is the 1-6 fragment of the protease-activated receptor 3 (PAR-3), which induces ERK activation in human carcinoma cells endogeneously expressing PAR1 and PAR3. This effect is completely abolished by single alanine substitution at positions 3, 4 and 6 in the peptide. PAR-3 allosterically regulates PAR1 signaling by receptor dimerization governing increased endothelial permeability. Targeting of PAR3 may mitigate the effects of PAR1 in activating endothelial responses such as vascular inflammation. However this peptide does not affect VEGF release or expression.
溶解度
分子量
647.73
化学式
C29H45N9O8
存储条件
Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents
Figures
Reference
H.Ishihara et al., Nature, 386, 502 (1997) K.K. Hansen et al., Immunology, 112, 183 (2004)
This peptide is a murine PAR-3-derived tethered ligand sequence which does not activate PAR-3, but rather activates PAR-1 and PAR-2, either in Jurkat T-cells or in other PAR-expressing cells. PAR3 Tethered Ligand (Murine) / Activates PAR1 and PAR2
溶解度
分子量
646.75
化学式
C29H46N10O7
存储条件
Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents
Figures
Reference
K.K.Hansen et al., Immunology, 112, 183 (2004) Kaufmann, R. et al. Regul. Pept. 125, 61( 2005) McLaughlin, J. et al. PNAS 104, 5662 (2007) Arisato, T. et al. Cell. Mol. Life Sci. 60, 1420 (2003)
This peptide is the amino terminus of the fourth protease-activated receptor (PAR4) after the cleavage at Arg47/Gly48 was tested for its ability to stimulate COS cells expressing PAR4. The peptide readily activated both wild-type and mutant PAR4 (R47A) at 500 碌M, whereas thrombin and trypsin only activate the wild-type PAR4.
溶解度
分子量
576.61
化学式
C25H36N8O8
存储条件
Store at -20°C. Keep tightly closed. Store in a cool dry place.
PAR-4 (1-6)) amide |Protease Activated Receptor-4 (1-6) amide, human
编码
[245443-51-0]
别名
PAR-4 (1-6)) amide |Protease Activated Receptor-4 (1-6) amide, human
纯度
80%,90%,95%,98%,99%
重量
1mg,5mg,10mg,50mg,100mg,1g
序列(单字母缩写)
GYPGQV-NH2
序列(三字母缩写)
H-Gly-Tyr-Pro-Gly-Gln-Val-NH2
基本描述
Protease-activated receptor-4 activating peptide derived from murine PAR-4. This peptide was able to cause rat platelet aggregation with an EC50 value of 40 碌M. Its effect on leucocyte rolling and adherence points at a role of PAR-4 in mediating proinflammatory processes. The family of at least seven transmembrane G protein coupled receptors includes at least four receptor subtypes (PAR1, PAR2, PAR3, PAR4). PAR4 activating peptides, GYPGKF-NH2 and AYPGKG-NH2, produce concentration dependent contractile effects on the muscle.
溶解度
分子量
618.69
化学式
C28H42N8O8
存储条件
Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents
Figures
Reference
M.D.Hollenberg et al., Can. J. Physiol. Pharmacol., 77, 458 (1999) M.Tognetto et al., Br. J. Pharmacol., 139, 21 (2003) M.D.Hollenberg and S.Houle, Swiss Med. Wkly., 135, 425 (2005)
This peptide based on the proteolytically-revealed tethered ligand sequence of the murine proteinase-activated receptor-4 (PAR-4) was shown to cause platelet aggregation with an EC50 value of about 15 碌M. PAR-4 agonist peptide stimulates thromboxane production by human platelets with the maximal response to this agonist being approximately half of that observed after maximal thrombin stimulation.
溶解度
分子量
666.78
化学式
C33H46N8O7
存储条件
Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents
Figures
Reference
N.Vergnolle, J. Immunol., 163, 5064 (1999) M.D.Hollenberg and M.Saifeddine, Can. J. Physiol. Pharmacol., 79, 439 (2001) Fang, J. et al. Blood 106, 2671(2005) Mulè, F. et al. Gut. 53, 229 (2004) Sekiguchi, F. et al. Drug Design Rev. 1, 287 (2004) Faruqi, T. et al. J. Biol. Chem. 275, 19728 (2000) Greenberg, D. Biochem. 42, 2702 (2003)
This PAR-4 activating peptide caused secretion and aggregation of PAR-3 deficient mouse platelets.
溶解度
分子量
619.68
化学式
C28H41N7O9
存储条件
Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents
Figures
Reference
W.-F.Xu et al., Proc. Natl. Acad. Sci. USA, 95, 6642 (1998) H.Andersen et al., Proc. Natl. Acad. Sci. USA, 96, 11189 (1999) R.Kaufmann et al., J. Cancer Res. Clin. Oncol., 126, 91 (2000)