标签归档：lip

Chol-CTPP

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Chol-CTPP 

Chol-CTPP 是一种对血脑屏障 (BBB) 和胶质瘤 （glioma） 细胞具有双重靶向作用的配体，能与 Chol-TPP 合成Lip-CTPP 。 Lip-CTPP 是发挥阿霉素 (DOX) 和氯胺达明 (LND) 联合抗胶质瘤 (anti-glioma) 作用的潜在载体。Lip-CTPP 可提高对肿瘤细胞增殖、迁移和侵袭的抑制率，促进细胞凋亡 (apoptosis) 和坏死 (necrosis)，干扰线粒体功能。

Chol-CTPP

Chol-CTPP Chemical Structure

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

Chol-CTPP is a ligand with dual targeting effect on blood-brain barrier (BBB) and glioma cells. Lip-CTPP can be gained by Chol-CTPP and another mitochondria targeting ligand (Chol-TPP). Lip-CTPP is a promising potential carrier to exert the anti-glioma effect of doxorubicin (DOX) and lonidamine (LND) collaboratively. Lip-CTPP elevates the inhibition rate of tumor cell proliferation, migration and invasion, promote apoptosis and necrosis, and interfere with mitochondrial function[1].

IC50 & Target

Apoptosis, ROS[1]
体外研究
(In Vitro)

Lip-CTPP shows satisfying cellular uptake and mitochondrial uptake[1].
Lip-CTPP (0-20 µg/mL DOX and LND, 24 h) shows cytotoxicity and induces apoptosis in C6 cells[1].
Lip-CTPP inhibits intracellular ATP production and has the most severe damage on the membrane potential of mitochondria[1].
Lip-CTPP possesses excellent potential to induce ROS generation[1].
Lip-CTPP (0.5 µg/mL DOX, 48 h) exhibits strong inhibitory effect both on cell migration and invasion[1]

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay[1]

Cell Line: C6 cells
Concentration: 0.1, 0.5, 2.5, 5, 10, and 20 µg/mL of DOX and LND
Incubation Time: 24 h
Result: Showed cytotoxicity on C6 cells in a concentration-dependent manner.

Apoptosis Analysis[1]

Cell Line: C6 cells
Concentration: 0.5 µg/mL DOX and LND
Incubation Time: 24 h
Result: Performed excellent lethality on C6 cells and the apoptosis and necrosis rate is 3.4 times that of Free DOX + LND.

Cell Invasion Assay[1]

Cell Line: C6 cells
Concentration: 0.5 µg/mL DOX
Incubation Time: 48 h
Result: Obviously restricted the invasion of C6 cells.

体内研究
(In Vivo)

Lip-CTPP (3 mg/kg DOX and LND; i.v.; once on day 4, 7, 10 and 13) induces glioma cells apoptosis and inhibits tumor growth[1].
Lip-CTPP can slow down the clearance of free drugs and enhance tumor targeting properties[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Kunming mice (male, 20-25 g), 5 µL of C6 cells (2 × 108 cells/mL) were injected into the striatum[1]
Dosage: 3 mg/kg DOX and LND
Administration: Tail vein injection, once on day 4, 7, 10 and 13
Result: Increased the survival time, decreased tumor area and the density of tumor cells.
Animal Model: Kunming mice (20-25 g)[1]
Dosage: 10 mg/kg DOX and LND
Administration: Tail vein injection (Pharmacokinetics Analysis)
Result: Pharmacokinetic parameters of DOX in blood after administration (mean ± SD, n = 3)[1]

parameters AUC(0-t)
(µg/mL*min)		 MRT (min) Tmax (min) Cmax (µg/mL) t1/2 (min) Clz (L/min/kg)
Lip-CTPP 5901.90 ± 406.18 291.30 ± 1.18 30 23.31 ± 0.42 231.06 ± 43.35 1.68 ± 0.13


Pharmacokinetic parameters of DOX in brain after administration (mean ± SD, n = 3)[1]

parameters AUC(0-t)
(µg/mL*min)		 MRT (min) Tmax (min) Cmax (µg/mL) t1/2 (min) Clz (L/min/kg)
Lip-CTPP 1757.61 ± 19.35

分子量

2884.62

Formula

C144H263N3O53
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Jiaqi Lu, et al. Multiple targeted doxorubicin-lonidamine liposomes modified with p-hydroxybenzoic acid and triphenylphosphonium to synergistically treat glioma. Eur J Med Chem. 2022 Feb 15;230:114093.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Chol-CTPP

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Chol-CTPP 

Chol-CTPP 是一种对血脑屏障 (BBB) 和胶质瘤 （glioma） 细胞具有双重靶向作用的配体，能与 Chol-TPP 合成Lip-CTPP 。 Lip-CTPP 是发挥阿霉素 (DOX) 和氯胺达明 (LND) 联合抗胶质瘤 (anti-glioma) 作用的潜在载体。Lip-CTPP 可提高对肿瘤细胞增殖、迁移和侵袭的抑制率，促进细胞凋亡 (apoptosis) 和坏死 (necrosis)，干扰线粒体功能。

Chol-CTPP

Chol-CTPP Chemical Structure

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

Chol-CTPP is a ligand with dual targeting effect on blood-brain barrier (BBB) and glioma cells. Lip-CTPP can be gained by Chol-CTPP and another mitochondria targeting ligand (Chol-TPP). Lip-CTPP is a promising potential carrier to exert the anti-glioma effect of doxorubicin (DOX) and lonidamine (LND) collaboratively. Lip-CTPP elevates the inhibition rate of tumor cell proliferation, migration and invasion, promote apoptosis and necrosis, and interfere with mitochondrial function[1].

IC50 & Target

Apoptosis, ROS[1]
体外研究
(In Vitro)

Lip-CTPP shows satisfying cellular uptake and mitochondrial uptake[1].
Lip-CTPP (0-20 µg/mL DOX and LND, 24 h) shows cytotoxicity and induces apoptosis in C6 cells[1].
Lip-CTPP inhibits intracellular ATP production and has the most severe damage on the membrane potential of mitochondria[1].
Lip-CTPP possesses excellent potential to induce ROS generation[1].
Lip-CTPP (0.5 µg/mL DOX, 48 h) exhibits strong inhibitory effect both on cell migration and invasion[1]

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay[1]

Cell Line: C6 cells
Concentration: 0.1, 0.5, 2.5, 5, 10, and 20 µg/mL of DOX and LND
Incubation Time: 24 h
Result: Showed cytotoxicity on C6 cells in a concentration-dependent manner.

Apoptosis Analysis[1]

Cell Line: C6 cells
Concentration: 0.5 µg/mL DOX and LND
Incubation Time: 24 h
Result: Performed excellent lethality on C6 cells and the apoptosis and necrosis rate is 3.4 times that of Free DOX + LND.

Cell Invasion Assay[1]

Cell Line: C6 cells
Concentration: 0.5 µg/mL DOX
Incubation Time: 48 h
Result: Obviously restricted the invasion of C6 cells.

体内研究
(In Vivo)

Lip-CTPP (3 mg/kg DOX and LND; i.v.; once on day 4, 7, 10 and 13) induces glioma cells apoptosis and inhibits tumor growth[1].
Lip-CTPP can slow down the clearance of free drugs and enhance tumor targeting properties[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Kunming mice (male, 20-25 g), 5 µL of C6 cells (2 × 108 cells/mL) were injected into the striatum[1]
Dosage: 3 mg/kg DOX and LND
Administration: Tail vein injection, once on day 4, 7, 10 and 13
Result: Increased the survival time, decreased tumor area and the density of tumor cells.
Animal Model: Kunming mice (20-25 g)[1]
Dosage: 10 mg/kg DOX and LND
Administration: Tail vein injection (Pharmacokinetics Analysis)
Result: Pharmacokinetic parameters of DOX in blood after administration (mean ± SD, n = 3)[1]

parameters AUC(0-t)
(µg/mL*min)		 MRT (min) Tmax (min) Cmax (µg/mL) t1/2 (min) Clz (L/min/kg)
Lip-CTPP 5901.90 ± 406.18 291.30 ± 1.18 30 23.31 ± 0.42 231.06 ± 43.35 1.68 ± 0.13


Pharmacokinetic parameters of DOX in brain after administration (mean ± SD, n = 3)[1]

parameters AUC(0-t)
(µg/mL*min)		 MRT (min) Tmax (min) Cmax (µg/mL) t1/2 (min) Clz (L/min/kg)
Lip-CTPP 1757.61 ± 19.35

分子量

2884.62

Formula

C144H263N3O53
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Jiaqi Lu, et al. Multiple targeted doxorubicin-lonidamine liposomes modified with p-hydroxybenzoic acid and triphenylphosphonium to synergistically treat glioma. Eur J Med Chem. 2022 Feb 15;230:114093.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Chol-CTPP

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Chol-CTPP 

Chol-CTPP 是一种对血脑屏障 (BBB) 和胶质瘤 （glioma） 细胞具有双重靶向作用的配体，能与 Chol-TPP 合成Lip-CTPP 。 Lip-CTPP 是发挥阿霉素 (DOX) 和氯胺达明 (LND) 联合抗胶质瘤 (anti-glioma) 作用的潜在载体。Lip-CTPP 可提高对肿瘤细胞增殖、迁移和侵袭的抑制率，促进细胞凋亡 (apoptosis) 和坏死 (necrosis)，干扰线粒体功能。

Chol-CTPP

Chol-CTPP Chemical Structure

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

Chol-CTPP is a ligand with dual targeting effect on blood-brain barrier (BBB) and glioma cells. Lip-CTPP can be gained by Chol-CTPP and another mitochondria targeting ligand (Chol-TPP). Lip-CTPP is a promising potential carrier to exert the anti-glioma effect of doxorubicin (DOX) and lonidamine (LND) collaboratively. Lip-CTPP elevates the inhibition rate of tumor cell proliferation, migration and invasion, promote apoptosis and necrosis, and interfere with mitochondrial function[1].

IC50 & Target

Apoptosis, ROS[1]
体外研究
(In Vitro)

Lip-CTPP shows satisfying cellular uptake and mitochondrial uptake[1].
Lip-CTPP (0-20 µg/mL DOX and LND, 24 h) shows cytotoxicity and induces apoptosis in C6 cells[1].
Lip-CTPP inhibits intracellular ATP production and has the most severe damage on the membrane potential of mitochondria[1].
Lip-CTPP possesses excellent potential to induce ROS generation[1].
Lip-CTPP (0.5 µg/mL DOX, 48 h) exhibits strong inhibitory effect both on cell migration and invasion[1]

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay[1]

Cell Line: C6 cells
Concentration: 0.1, 0.5, 2.5, 5, 10, and 20 µg/mL of DOX and LND
Incubation Time: 24 h
Result: Showed cytotoxicity on C6 cells in a concentration-dependent manner.

Apoptosis Analysis[1]

Cell Line: C6 cells
Concentration: 0.5 µg/mL DOX and LND
Incubation Time: 24 h
Result: Performed excellent lethality on C6 cells and the apoptosis and necrosis rate is 3.4 times that of Free DOX + LND.

Cell Invasion Assay[1]

Cell Line: C6 cells
Concentration: 0.5 µg/mL DOX
Incubation Time: 48 h
Result: Obviously restricted the invasion of C6 cells.

体内研究
(In Vivo)

Lip-CTPP (3 mg/kg DOX and LND; i.v.; once on day 4, 7, 10 and 13) induces glioma cells apoptosis and inhibits tumor growth[1].
Lip-CTPP can slow down the clearance of free drugs and enhance tumor targeting properties[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Kunming mice (male, 20-25 g), 5 µL of C6 cells (2 × 108 cells/mL) were injected into the striatum[1]
Dosage: 3 mg/kg DOX and LND
Administration: Tail vein injection, once on day 4, 7, 10 and 13
Result: Increased the survival time, decreased tumor area and the density of tumor cells.
Animal Model: Kunming mice (20-25 g)[1]
Dosage: 10 mg/kg DOX and LND
Administration: Tail vein injection (Pharmacokinetics Analysis)
Result: Pharmacokinetic parameters of DOX in blood after administration (mean ± SD, n = 3)[1]

parameters AUC(0-t)
(µg/mL*min)		 MRT (min) Tmax (min) Cmax (µg/mL) t1/2 (min) Clz (L/min/kg)
Lip-CTPP 5901.90 ± 406.18 291.30 ± 1.18 30 23.31 ± 0.42 231.06 ± 43.35 1.68 ± 0.13


Pharmacokinetic parameters of DOX in brain after administration (mean ± SD, n = 3)[1]

parameters AUC(0-t)
(µg/mL*min)		 MRT (min) Tmax (min) Cmax (µg/mL) t1/2 (min) Clz (L/min/kg)
Lip-CTPP 1757.61 ± 19.35

分子量

2884.62

Formula

C144H263N3O53
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Jiaqi Lu, et al. Multiple targeted doxorubicin-lonidamine liposomes modified with p-hydroxybenzoic acid and triphenylphosphonium to synergistically treat glioma. Eur J Med Chem. 2022 Feb 15;230:114093.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

微生物质素过氧化物酶（LiP）ELISA检测试剂盒BS-4858

发表于由

微生物质素过氧化物酶（LiP）ELISA检测试剂盒

  • 产品型号：BS-4858
  • 简要描述：微生物质素过氧化物酶（LiP）ELISA检测试剂盒金畔生物公司供应：ELISA试剂盒,荧光定量PCR耗材，移液器吸嘴，微量离心管，进口冻存管，细胞培养皿，培养板，培养瓶，吸头，仪器及手套，色谱耗材，针头过滤器。
产品咨询在线客服
  • 产品简介

微生物质素过氧化物酶(LiP)ELISA检测试剂盒金畔生物公司供应：ELISA试剂盒,血清，荧光定量PCR耗材，移液器吸嘴，微量离心管，进口冻存管，细胞培养皿，培养板，培养瓶，吸头，仪器及手套，色谱耗材，针头过滤器。

规格：96T/48T

BS-4858         微生物质素过氧化物酶(LiP)ELISA试剂盒

检测种属：人、大小鼠、豚鼠、兔子、猪、犬、牛羊、鸡鸭、猴ELISA试剂盒等种属。

保存条件及有效期：

1、试剂盒保存：2-8℃。

2、有效期：6个月

标记物：血清、血浆、组织匀浆等

【测试种属】犬、大小鼠、人、豚鼠、兔子、牛羊、猪、鸡鸭elisa试剂盒等种属
【储存方式】：2-8℃
【检测目的】用于测定血清，血浆及相关液体等样本。例如适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本。
【用途】科研实验，不用于临床诊断。

微生物质素过氧化物酶(LiP)ELISA检测试剂盒

样本处理及要求：

1. 血清：室温血液自然凝固10-20分钟，离心20分钟左右(2000-3000转/分)。仔细收集上清，保存过程中如出现沉淀，应再次离心。

2. 血浆：应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂，混合10-20分钟后，离心20分钟左右(2000-3000转/分)。仔细收集上清，保存过程中如有沉淀形成，应该再次离心。

3. 尿液：用无菌管收集，离心20分钟左右(2000-3000转/分)。仔细收集上清，保存过程中如有沉淀形成，应再次离心。胸腹水、脑脊液参照实行。

4. 细胞培养上清：检测分泌性的成份时，用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时，用PBS(PH7.2-7.4)稀释细胞悬液，细胞浓度达到100万/ml左右。通过反复冻融，以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成，应再次离心。

5. 组织标本：切割标本后，称取重量。加入一定量的PBS，PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4)，用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测，其余冷冻备用。

6. 标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融.

7. 不能检测含NaN3的样品，因NaN3抑制辣根过氧化物酶的(HRP)活性。

微生物质素过氧化物酶(LiP)ELISA试剂盒操作步骤：

微生物质素过氧化物酶（LiP）ELISA检测试剂盒BS-4858

检测试剂盒组成：
试剂盒组成48 孔配置96 孔配置保存
说明书1 份1 份
封板膜2 片(48)2 片(96)
密封袋1 个1 个
酶标包被板1×481×962-8℃保存
标准品：540μg/L0.5ml×1 瓶0.5ml×1 瓶2-8℃保存
标准品稀释液1.5ml×1 瓶1.5ml×1 瓶2-8℃保存
酶标试剂3 ml×1 瓶6 ml×1 瓶2-8℃保存
样品稀释液3 ml×1 瓶6 ml×1 瓶2-8℃保存
显色剂 A 液3 ml×1 瓶6 ml×1 瓶2-8℃保存
显色剂 B 液3 ml×1 瓶6 ml×1 瓶2-8℃保存
终止液3 ml×1 瓶6 ml×1 瓶2-8℃保存
浓缩洗涤液(20ml×20 倍)×1 瓶(20ml×30 倍)×1 瓶2-8℃保存

3ML塑料刻度巴氏吸管,滴量52ul,长178mm,单支灭菌包装

3ML塑料刻度巴氏吸管,滴量48ul,长160mm,单支灭菌包装

3ML塑料刻度巴氏吸管,滴量52ul,长162mm,单支灭菌包装

2ML塑料刻度巴氏吸管,滴量48ul,长150mm,单支灭菌包装

1ml塑料刻度巴氏吸管,滴量45ul,长145mm,单支灭菌包装

3ML塑料刻度巴氏吸管,滴量48ul,长160mm,非灭菌

3ML塑料刻度巴氏吸管,滴量52ul,长162mm,非灭菌

2ML塑料刻度巴氏吸管，滴量48ul，长150mm，非灭菌

1ml塑料刻度巴氏吸管，滴量45ul,长145mm,非灭菌

塑料刻度巴氏吸管

T-75, 250ml细胞培养瓶,密封盖

T-75, 250ml细胞培养瓶，斜口，密封盖，螺口，灭菌

进口PCR板,200μl96孔无裙边透明PCR板

适用于384孔PCR板硅胶片,圆形孔,无菌

200μl透明96孔无裙边PCR板

 

产品用途：可用于科研实验,不用于临床治疗！